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Citations for Leica :
401 - 450 of 494 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... fixed brains were embedded in 3% agarose and sectioned at 50 μm thickness with a vibrating microtome (Leica VT1000S Microtome). Free floating sections were incubated in 1% hydrogen peroxide for 15 minutes then incubated with anti-ATP synthase subunit C (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were either embedded in 3% agarose for coronal sectioning at 150 µm thickness on a vibrating microtome (VT1000S; Leica) or cryoprotected samples and sectioned coronally at 20 µm on a Leica CM3050 cryostat ...
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bioRxiv - Plant Biology 2024Quote: ... the eYFP fluorescence of fusion proteins was assayed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8). For the Split–LUC assay ...
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bioRxiv - Cell Biology 2024Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...
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bioRxiv - Microbiology 2024Quote: ... the eYFP fluorescence of fusion proteins was analyzed 2–3 days after infiltration using a confocal microscope (Leica TCS SP8).
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bioRxiv - Molecular Biology 2023Quote: ... Zi-1 = 75.61μm) of pancreatic beta cells using the microscope’s TXR filter and a 40X objective (Leica HCX APO L U-V-I 0.80 W), at 20 msec exposures ...
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bioRxiv - Pathology 2023Quote: ... Diluted primary antibody (CST #8242 1:800; CST #99940, 1:150; CST #9718, 1:500; Abcam ab107099, 1:X; Leica P53-CM5P-L 1:200) was applied and slides were incubated at 4°C overnight ...
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bioRxiv - Biophysics 2021Quote: ... These dauer samples were then transferred to carriers of 3 mm diameter and 0.1 mm depth and rapidly frozen in a high-pressure freezing machine (Leica, EM ICE). For automated freeze substitution ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed 6 hours and 3 days after seeding and imaged in brightfield using a Leica DMI 4000 B microscope (Leica Microsystems).
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bioRxiv - Cell Biology 2019Quote: Images of ZEB1 transgenic ZEB1+/+ and ZEB1+/- cell cultures at day 1 (sub-confluent) and at day 3 post-seeding (confluent) were acquired using the Leica DMIL LED inverted microscope (Leica Microsystems) and the N PLAN L 20x/0.35 PH1 objective ...
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bioRxiv - Neuroscience 2021Quote: ... i.p.) at P120 and P123 (after 3 days of monocular deprivation) Images were visualized using a custom Leica microscope (Leica Microsystems). Red light illumination was provided by 8 individually addressable LEDs (WS2812 ...
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bioRxiv - Cell Biology 2021Quote: ... was assembled on a Narishige micromanipulator model MM-3 (Narishige group) and was placed on the magnetic board of a Leica MS5 dissection microscope (Leica Biosystems), using a Narishige GJ-8 magnetic stand (Narishige group) ...
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bioRxiv - Plant Biology 2020Quote: ... and germlings was measured after 15 min of exposure to NCR044.1 (50 μL, 3 μM) using fluorescence confocal microscopy (Leica SP8-X) with an excitation and emission wavelength of 488 and 508-544 nm ...
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bioRxiv - Cell Biology 2021Quote: ... whereas samples for STEM were attached to a sticky Post-It-Note (as described in (31)) and coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The STEM samples were then washed with Buffered Oxide Etchant (diluted 6:1 with surfactant ...
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bioRxiv - Cell Biology 2021Quote: ... around 1.5 µl of concentrated purified schizont pellet was transferred into aluminium or gold carriers (3 mm diameter, 100 or 200 µm depth; Leica Microsystems) and high pressure frozen with EM ice (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... The glycolytic and oxidative portions of the TA muscles of 5 mice in the CTR group and 5 mice in the Crush - 3 days group were microdissected with a laser microdissection system (Leica LMD6) and recovered in RNAlater reagent (QIAGEN).
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agarose (3% in Water) and 70 µm coronal sections were cut with a VT1000S vibratome (Leica Biosystems). Every second section ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with a mixture of oxygen (1L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Cell Biology 2019Quote: ... for 2 days at 4 °C and then transferred to 70% alcohol for 3-5 days before processed by using 4-hour tissue processing setting with Leica ASP6025 (Leica Biosystems). Placentas were embedded in paraffin (HistoCore Arcadia H and Arcadia C ...
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bioRxiv - Physiology 2020Quote: ... Finally, sections were incubated with DAB (3,3’-diaminobenzidine, Vector) for 3 min and counterstained with Gill’s hematoxylin (Leica, Buffalo Grove, IL) for 30 sec ...
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bioRxiv - Plant Biology 2020Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Microbiology 2021Quote: ... containing defined regions of the bacterial colonies were cut out and inverted on lecithin-coated 3-mm specimen carriers for high pressure freezing in a high- pressure freezing machine (Leica, ICE) with or without 10% polyvinylpyrrolidone as a filler (Δeps biofilms and one of the WT samples were frozen without filler) ...
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bioRxiv - Cell Biology 2019Quote: ... at least 3 adult worms for each condition were fixed using high pressure freezing (Leica EM PACT2, Leica Microsystems, Vienna, Austria) in 20% BSA (Sigma-Aldrich Co. ...
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bioRxiv - Plant Biology 2021Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Cell Biology 2021Quote: ... microscopy was performed with the 100×/1.4 Oil HC PL APO CS2 STED White objective of a Leica TCS SP8 3× STED microscope (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
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bioRxiv - Plant Biology 2022Quote: ... were infiltrated in Nicotiana benthamiana leaves for transient expression and viewed after 3 days under confocal microscope Leica TCS SP5 (Leica Microsystems) equipped with appropriate lasers (514 nm -527 nm and 532 nm-588 nm for YFP and RFP ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome, Leica Biosystems). The tissue sections were fixed in a glass slide and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2023Quote: ... The paraffin embedded clots were cut in 3 µm thick sections with a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany) and placed on silanized glass slides ...
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bioRxiv - Neuroscience 2023Quote: ... cluster of differentiation 3 (CD3) and CD68 immunostaining was performed using a fully automated staining system (BOND-III; Leica Biosystems, UK). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections of 3 µm thickness were cut at the level of the hippocampus using a microtome (Leica Microsystems, Wetzlar, Germany). For immunohistochemistry either paraffin sections that were washed with 0.5% sodium azide (2 × 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 1h incubation in the same buffer containing the primary probes and a 3 min heat incubation step in a ThermoBrite slide hybridization system (Leica Biosystems), either at 75°C or 86°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Image acquisition was performed as z-stack images of 250 μm in depth in 3 separate channels using a Leica SP8 confocal microscope (Leica, Germany).
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bioRxiv - Biophysics 2023Quote: ... and then glued onto Leit-tabs (Plano, Germany) mounted on aluminum stubs (Plano, Germany) and sputter-coated with a 3 nm thin gold layer (ACD600, Leica, Austria). SEM images were acquired with a Zeiss Auriga FEG-SEM (Zeiss ...
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bioRxiv - Plant Biology 2023Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Plant Biology 2024Quote: ... and Antirrhinum majus stained with SR2200 and TO-PRO-3 iodide or DAPI was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Cell Biology 2024Quote: ... was transferred to B-type planchettes (3 mm) and rapidly frozen with a Leica EM ICE high-pressure freezer (Leica Microsystems). The frozen specimens were freeze-substituted in anhydrous acetone containing 2% osmium tetroxide (OsO4 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed 3 times with PBS and imaged using a Leica SP8 laser scanning confocal microscope (Leica Microsystems, Heidelberg, Germany) using a 63x water objective ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunohistochemical (IHC) staining for Estrogen receptor (ER) was conducted using the Leica Bond-3 automated stainer platform (Leica, Buffalo Grove, IL). Following antigen retrieval using a citrate-based pH 6 epitope retrieval solution ...
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bioRxiv - Molecular Biology 2023Quote: ... three z-stacks of 75 optical sections each (ΔZi, Zi-1 = 151.75μm) were acquired of the bigger liver lobe using a 20X objective (Leica HCX APO L U-V-I 0.80 W). One z-stack was in bright field and acquired at 20 msec exposures ...
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bioRxiv - Biophysics 2019Quote: ... Then a bulk of muscle was washed 2 to 3 times in cold PBS and fixed on the specimen holder of VT1200S vibrating blade microtome (Leica Biosystems, US). The buffer tray of microtome was ice bathed and filled with cold PBS while slicing ...
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bioRxiv - Cancer Biology 2019Quote: ... homogeneous and mixed spheroids were generated and imaged repeatedly over time (2-3 times per week) using brightfield microscopy (4x objective; 0.3 NA, Leica DM IRBE, Hamamatsu). Spheroid volumes were calculated using SpheroidSizer58.
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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bioRxiv - Microbiology 2020Quote: ... The embedded tissues were sectioned to 3 μm-thick sections and stained with Haematoxylin-Erythrosin-Saffron (HES) before visualizing under a Leica DMRBE light microscope (Leica Microsystems, Germany). The stained sections were scanned with a Hamamatzu NanoZomer S60 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Cancer Biology 2019Quote: ... Larvae were washed 3 times with PBS 1x and livers were carefully dissected with the use of forceps on a stereomicroscope (Leica MZ 9.5). After dissection ...