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Citations for Leica :
301 - 350 of 494 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... larvae were imaged in 3% methylcellulose on a binocular microscope using Leica Application Suite (LAS 3.3, Leica). After imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 3% agarose and cut into 100μm thick sections using a VT1000S vibratome (Leica). Sections were incubated O/N in primary antibody solution containing 5% NGS and 0.3% triton ...
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bioRxiv - Immunology 2024Quote: ... Nine TLS+ Glioma FFPE sections (3 µm) were generated using the Leica SM 2000R microtome (Leica Microsystems), and incubated at 37 C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The excitation beam was focused with a water-dipping objective lens (Nikon CFI75 16× 0.8NA or Leica HC FLUOTAR L 25x 0.95NA). The average power was 100-150 mW at the sample ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were mounted for imaging on a Leica SP8 confocal with a dipping immersion objective (Leica HCX IRAPO L, 25X, N.A. 0.95). Z-stacks were taken every 10 µm ...
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bioRxiv - Plant Biology 2020Quote: ... containing 1 g/l calcofluor white M2R and 0.5 g/l evans blue and then mounted on a TCS SP5 II CLSM (Leica Microsystems, Vienna, Austria). As emission source a 405 nm UV diode was used ...
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bioRxiv - Biochemistry 2021Quote: ... 4 μL sample were applied onto a grid held in the sample grid chamber of a Leica EM GP instrument (Leica Microsystem Inc.) cooled at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... T1 seeds from plants transformed with pSS01_35Spro:LIP1-GFP and pB7WG2_35Spro:LIP1 were harvested and selected either with 240 mg/L glufosinate ammonium solution (Basta) or screened for fluorescence on a stereomicroscope (Leica M165 FC) equipped with a GFP filter (excitation ...
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bioRxiv - Cancer Biology 2024Quote: The assay consisted of the following antibodies and corresponding dilution factor: CK AE1/AE3 (Leica Biosystems, ref. AE1/AE3-601-L-CE) was applied at 1:250 for 28 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... applied onto glass coverslips precoated by poly-L-lysine and immediately analyzed by a Leica TCS SP8 confocal microscope (Leica, Wetzlar, Germany) equipped with a water immersion objective HC PL APO CS2 with magnification 63x ...
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bioRxiv - Neuroscience 2020Quote: ... embedded with agarose 3% in PBS 0.01M and cut in slices of 80μm of thickness in a vibratome (Leica). Slices were incubated for 1h at room temperature in a blocking solution containing 1% BSA and 0.3% Triton X-100 in PBS 0.01M ...
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bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Immunology 2021Quote: ... Tissues were routinely processed for histopathology on a Leica Peloris 3 Tissue Processor (Leica Biosystems, Buffalo Grove, IL), embedded in paraffin ...
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bioRxiv - Microbiology 2021Quote: ... Confocal imaging was performed with a Leica TCS SP8 STED 3× laser scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 100×/1.4 Oil HC PL APO CS2 STED White objective ...
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bioRxiv - Neuroscience 2022Quote: ... then following fixation they were imaged with an inverted confocal microscope with the laser power increasing for 0.2 to 3% with 0.2% intervals (Extended Data Fig. 1a, b, SP8 TCS with HyD detectors, Leica). Laser power was linearly controlled by ATOF ...
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bioRxiv - Plant Biology 2022Quote: ... leaves were harvested at 3 dpi for aniline blue/calcofluor staining and analysis by epi-fluorescence microscopy (Leica DM6B ...
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bioRxiv - Plant Biology 2022Quote: ... GUS-stained leaves were embedded in 3% agarose and sectioned with a vibrating blade microtome (Leica VT1000 S). Images were taken using ZEISS Axio Observer.
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bioRxiv - Neuroscience 2023Quote: ... the ISH-mounted specimen was first loaded into a pre-tilted specimen block (Leica; Figure 3 J&K). The block was then placed into a Leica VCT-100 cryo-transfer system under liquid nitrogen to facilitate transfer into the pre-cooled cryo-FIB/SEM cryo-substage.
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bioRxiv - Plant Biology 2022Quote: ... Fixed and stained roots were mounted onto microscopic slides and cleared with chloral hydrate:glycerol:water (8:3:1 ratio) before imaging with the Thunder Imaging Systems Microscope (Leica).
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome ...
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bioRxiv - Neuroscience 2024Quote: ... Videos of Day 3 adult worms for at least 20 seconds was recorded using stereo-zoom microscope (Leica) 28 ...
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bioRxiv - Cell Biology 2023Quote: ... The cryosection ribbon (∼3 mm long) was then attached to the grid by operating the Crion (Leica Microsystems) in “charge” mode for ∼30 seconds (Pierson et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in blocks of 3% agarose and sectioned in 100 µm slices on a vibrating blade microtome (Leica) or mounted as whole droplets.
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bioRxiv - Physiology 2023Quote: ... and incubated for 3 x 1 h in Xylene in an automatized system (TP1020 Tissue Processor, Leica Biosystems). Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2021Quote: ... Red and cyan fluorescence were detected through the objective (Olympus LUMPLFLN 60XW, 1.0 NA, or Leica HC FLUOTAR L 25x/0.95 W VISIR) and through the oil immersion condenser (numerical aperture 1.4 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 3 μl were added to the plasma cleaned grids and subsequently plunge-frozen in liquid ethane using a Leica EMGP (Leica microsystems, Wetzlar, Germany). Blotting time was set to 1s with the chamber temperature and humidity being at 20°C and 85% ...
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bioRxiv - Cancer Biology 2021Quote: ... Each wash was performed for 3 min at room temperature with repetitive dipping using a linear stainer (Leica, #ST4020). Slides were transferred for heat-induced epitope retrieval to a PT module (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: Fertilized ovaries from B73 plants were collected 3 days after pollination (DAP) and sliced using a Vibratom (Leica VT1000E) to create 200 to 225 μm sections ...
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bioRxiv - Cancer Biology 2020Quote: ... two sections of formalin-fixed paraffin-embedded tissues of FMC with 3 µm thickness (Microtome Leica RM2135, Newcastle, UK) were prepared ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Neuroscience 2021Quote: ... Three (3) brains per group were later sectioned with a cryostat (Cryocut 1800 Cryostat, Leica Biosystems, Vista, CA, USA) at 25 μm thickness in the coronal plane ...
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bioRxiv - Immunology 2020Quote: ... Lung tissues were embedded in 3% LMA and 100μm-thick sections were cut at the vibratome (Leica VT 1200S). Sections were placed on microscope slides ...
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bioRxiv - Plant Biology 2020Quote: ... cinerea conidia and germlings exposed to 3 μM NCR044.1 was also imaged using fluorescence confocal microscopy (Leica SP8-X)39 with an excitation and emission wavelength of 480 and 510-566 nm ...
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bioRxiv - Genomics 2021Quote: ... Formalin-fixed agarose/paraffin-embedded sections (3-5µm) were manually cut from blocks with a microtome (Leica RM 2145) and transferred to glass slides (Superfrost ...
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bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2 ...
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bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...
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bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.
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bioRxiv - Biophysics 2022Quote: ... 300 mesh grids were coated with a continuous carbon layer of 3 nm in sputter coater EM ACE600 (Leica). The continuous carbon layer was first deposited on mica support of 25 x 25 mm (Cat Nr 71850-11 ...
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bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 2 to 3 days after infiltration using a SP8 laser scanning microscope (Leica Microsystems GmbH) with LAS AF and SymPhoTime (PicoQuant ...
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bioRxiv - Biophysics 2023Quote: ... We image z-stacks around single cells with a cubic volume of (123μm)3 using confocal reflection microscopy (Leica DM 6000 CFS with HCX APO L 20x/1,00 W objective) ...
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bioRxiv - Molecular Biology 2024Quote: ... Images were captured at 2 min 30 sec intervals for 3 hours and processed using LAS X software (Leica).
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bioRxiv - Neuroscience 2020Quote: ... iAChSnFR fluorescence was detected by a photomultiplier tube (H11706-40, Hamamatsu) through a 25x objective lens (Leica, HCX IRAPO L 25x/ 0.95 W, Germany), a dichroic mirror (Semrock) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...