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Citations for Leica :
251 - 300 of 494 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: The flagellar filament sample (4.5 μl) was applied to glow-discharged lacey carbon grids and then plunge-frozen using an EM GP2 Plunge Freezer (Leica). The cryo-EM micrographs were collected on a 300 keV Titan Krios with a K3 camera at 1.11 Å per pixel and a total dose of 50 e-/Å2 ...
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bioRxiv - Biochemistry 2024Quote: Droplet fusion was recorded in mixtures containing 20 µM Loc1p and 0.5x mass ratio total HeLa RNA with the above-mentioned setup (HC PL Fluotar L 40x/0.6 PH2 objective on Leica DMi8) in movie mode ...
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bioRxiv - Biochemistry 2024Quote: ... Condensates were imaged by phase contrast microscopy using an HC PL Fluotar L 40x/0.6 PH2 objective on a Leica DMi8 (Leica, Germany). Experiments were performed at least three independent times.
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bioRxiv - Biophysics 2023Quote: ... around individual cells are recorded in bright-field and confocal reflection mode using a 20x water dip-in objective (HCX APO L 20x/1.00 W, Leica, Wetzlar). The acquisition time for an individual image stack is below 10 seconds ...
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bioRxiv - Cancer Biology 2023Quote: ... 5µm thick sections were cut using a Leica RM2125 RTS microtome and sectioned onto Poly-L-lysine coated slides (Leica Surgipath Apex Superior adhesive slides ...
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bioRxiv - Microbiology 2023Quote: ... islandicus REY15A grown under poor media (4.5 μl) was applied to glow-discharged lacey carbon grids and then plunge-frozen using an EM GP Plunge Freezer (Leica). The cryo-EMs were collected on a 300 keV Titan Krios with a K3 camera at 1.08 Å per pixel and a total dose of 50 e-/Å2 ...
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bioRxiv - Developmental Biology 2023Quote: Live samples were imaged using a Leica Stellaris 5 laser-scanning confocal microscope with a water HC FLUOTAR L VISIR 25X/0.95 objective and HyD S detector (Leica Microsystems). Larvae were dissected and pinned to expose ventral muscles and imaged live in ambient temperature while the larvae were maintained in ice-cold HL3.1 buffer ...
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bioRxiv - Microbiology 2023Quote: ... a small volume (5 μL) of the stained suspension is placed on a glass slide and examined under a confocal laser scanning microscope (Leica SP-5 Confocal Laser Scanning Microscope) ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then washed three times with 1X PBS and incubated with geminin-L-CE primary antibody (Leica Biosystems) for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were incubated in 3% hydrogen peroxide (Bond Polymer Refine Detection Kit DS9800, Leica Biosystems) for 15 mins to block endogenous peroxidases ...
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bioRxiv - Developmental Biology 2021Quote: ... or 1:300 (rabbit anti-cleaved caspase 3, Cell Signaling Technology, 9665S; rabbit anti-p53, Leica Microsystems ...
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bioRxiv - Evolutionary Biology 2021Quote: ... embedded in 3% LM-agarose (Roth) and cut 250 µm-thick with a vibratome (Leica VT1000S). Slices were then transferred on Millicell inserts (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: Broad-band autofluorescence was acquired from sections cut at 3 μm using X20 objective (Leica DM550B). Sections were excited at 458 nm and fluorescence emission captured above 475 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... and glypican 3 (Cell Marque, #261M) was detected using BOND Polymer Refine Detection (Leica Biosystems, #DS9800), including a hematoxylin counterstain to visualize nuclei.
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bioRxiv - Microbiology 2019Quote: ... phase-3 position in an upright bright field-phase contrast microscope (DM-2500, Leica Microsystems, Germany). The viewer was unaware of the expected outcome of the respective stages of the samples to avoid any biasness ...
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bioRxiv - Neuroscience 2019Quote: ... and kynurenic acid 1 for 2-3 min and then glued on a vibratome VT1000S (Leica). The slicing chamber was filled with the same ice-cold aCSF ...
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bioRxiv - Developmental Biology 2020Quote: ... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
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bioRxiv - Cell Biology 2022Quote: ... and 3% water in acetone) and placed in an automated freeze substitution machine (AFS2, Leica Microsystems). A freeze substitution protocol was used as previously described16 ...
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bioRxiv - Neuroscience 2023Quote: High-pressure freezing: Reprogrammed cortical neurons were seeded and grown on 3 mm sapphire discs (Leica) until PID30 ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated with primary antibodies (Table 3) in Bond primary antibody diluent (AR9352, Leica) followed by secondary antibodies (Leica ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... followed by thin (∼3 nm) copper sputtering (Bal-tec MED 020, Leica Microsystemes SAS, Nanterre, France). Imaging was carried out using a Hitachi S800 FEG ...
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bioRxiv - Developmental Biology 2023Quote: Fixed brains were embedded in 3% noble agar gel and sectioned with the Vibratome VT1000S (Leica) at 50 μm thickness as free-floating sections ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue clearing was performed using the FluoClearBABB approach24 and whole mount images were then acquired using a SP8 microscope equipped with a white light laser and a HCX APO L 20x/0.95 IMM lens (Leica Microsystems, Germany). Imaging data was analyzed on a workstation (Thinkmate ...
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bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
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bioRxiv - Neuroscience 2022Quote: ... osmolarity 280-320 mOsm/l) was used both to keep tissue cold during dissection and as the bathing solution during vibratome slicing (Leica VT1200S). 0.4-1 mm-thick dorsal OB slices were taken by near-horizontal slicing ...
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bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
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bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
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bioRxiv - Plant Biology 2019Quote: ... Expression of the appropriate construct was analysed after 3 days by confocal laser-scanning microscopy (Leica SP8) with excitation lights of 405 nm for CFP and 488 nm for GFP.
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bioRxiv - Cell Biology 2020Quote: ... Images were prepared with the Leica Application Suite – Advanced Fluorescence (LAS-AF) Version 3 software (Leica Microsystems).
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bioRxiv - Biophysics 2019Quote: ... we imaged cilia dynamics with 3 different microscopes (two commercial spinning disk microscopes from 3i and Leica, and one 2-photon scanning microscope using the scanning artifact ...
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bioRxiv - Neuroscience 2020Quote: ... the Ca2+ signal within the SNs was captured during 3-min using Leica DM6000 microscope (Leica Microsystems) and processed with the software Fiji image J ...
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bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL) to a thickness of 30 µm ...
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bioRxiv - Cell Biology 2020Quote: ... The surface area of cardiomyocytes stained by MHC was determined with image analysis software (Leica Qwin 3) and calculated as the mean of 100 to 120 cells from randomly selected fields.
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bioRxiv - Cell Biology 2021Quote: ... were recorded and the maximal projection was deconvoluted with 3 iterations by the LAS AF 2.7.3.9 software (Leica).
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bioRxiv - Biochemistry 2021Quote: ... for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems). Datasets were collected at the NIH Multi-Institute Cryo-EM Facility (MICEF ...
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bioRxiv - Molecular Biology 2019Quote: ... A 30 second video recording was captured using a 3 MP DFC290 HD digital camera (Leica Microsystems) at a rate of 30 frames per second ...
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bioRxiv - Physiology 2020Quote: ... mexicanum gametes was imaged using FluoZin-3 and a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Leica 506224 5X objective ...
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bioRxiv - Neuroscience 2020Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Positive nuclei (Ki67) and stained area (cleaved caspase 3) were analyzed using the ImageScope software (Leica Biosystems). Quantification is average of 3 sections ...
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bioRxiv - Neuroscience 2021Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were sliced in 30 μm coronal sections into 3 series on a cryostat (Leica CM3050 S) and stored free-floating in antifreeze cryoprotectant [0.25 mM polyvinylpyrolidone ...
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bioRxiv - Cell Biology 2022Quote: Cells attached to 3-mm sapphire discs were cryo-immobilized using an EM ICE (Leica Microsystems, Austria) and 6-mm sapphire discs were frozen by using a Compact 03 high-pressure freezer (M ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm root segments were embedded in 3% agarose and sectioned using a vibratome (Leica VT1000 S) to produce 150-200 μM thickness sections ...
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bioRxiv - Neuroscience 2022Quote: ... They were embedded in 3% agar and 200 µm thick sections cut using vibratome (VT1000S, Leica, GmbH). Sections were mounted onto gelatin-coated slides ...
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bioRxiv - Developmental Biology 2024Quote: ... larvae were imaged in 3% methylcellulose on a binocular microscope using Leica Application Suite (LAS 3.3, Leica). After imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 3% agarose and cut into 100μm thick sections using a VT1000S vibratome (Leica). Sections were incubated O/N in primary antibody solution containing 5% NGS and 0.3% triton ...