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Citations for Leica :
201 - 250 of 494 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... was included in the internal solution and cells were bolus-loaded for 5 min and patched-off by gently retracting the patch pipette.The fluorescence signal was captured on a Leica DMLFSA confocal microscope with a 63x water-immersion objective (NA = 0.9, HCX APO L 63x/0.9 W U-V-I, Leica) using the Leica Confocal Software (Version 2.61) ...
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were stained manually with mouse anti-TTF1 (clone SPT24; TTF-1-L-CE, Leica, 1/400, 25’) and mouse anti-P40 (clone BC28 ...
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bioRxiv - Biophysics 2023Quote: Widefield fluorescence images were acquired using 20X air objective (HC PL FLUOTAR L PH1 20x, NA=0.4, Leica) mounted on a Leica DMi8 microscope ...
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bioRxiv - Neuroscience 2024Quote: ... The output was relayed to the back focal plane of the objective lens (HCX IRAPO L 25×, 0.95 NA W, 2.5 mm WD, Leica) through the scan lens (LSM54-1050 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were imaged in egg water on Leica SP8 and LAS X 2.01.14392 software with a HC FLUOTAR L 25x/0.95 W VISIR water immersion lens (Leica 15506374) at 0.75x zoom ...
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bioRxiv - Biophysics 2019Quote: ... cells were counted using a hemocytometer (Hausser Scientific) viewed in phase contrast on a Leica DMIL microscope with a 20x (Leica, N Plan, 0.35 NA) objective ...
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bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
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bioRxiv - Microbiology 2022Quote: ... covered with the flat side of a type B 3 mm aluminum platelet (Leica Microsystems), and was vitrified by high-pressure freezing using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were blocked on 3% agarose and sectioned using a VT1200 S vibratome (Leica Biosystems), to 40 μm sections ...
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bioRxiv - Cell Biology 2022Quote: ... intact gametocysts bathed in 3% BSA were subjected to high-pressure freezing (HPM100, Leica Microsystems), before freeze-substitution using a temperature-controlled chamber (AFS ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with 3% isoflurane and placed in a stereotaxic frame (Leica Microsystems Inc). For the craniotomy (centered at A/P −4.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... FFPE samples were sectioned in slices with 3 μm thickness (Microtome Leica RM135, Newcastle, UK) and mounted on a glass slide (SuperFrost Plus ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were sectioned at 3 μm thickness on a LeicaRM2145 microtome (Leica Microsystems, Nussloch, Germany) and stained in Richardson solution (1% methylene blue ...
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bioRxiv - Plant Biology 2021Quote: ... Observations were carried out every 2–3 days with an electronic binocular microscope (Leica M80) to monitor seed germination ...
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bioRxiv - Genetics 2022Quote: ... brains were embedded in 3% low-melting agarose and cut with a vibratome (VT1200S, Leica). 100µm thick sections were mounted on gelatine-coated slides and allow to dry for two days before staining ...
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bioRxiv - Microbiology 2022Quote: ... covered with the flat side of a 3-mm type B aluminum plate (Leica Microsystems) and vitrified by high-pressure freezing in a HPM100 system (Leica Microsystems) ...
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bioRxiv - Neuroscience 2019Quote: ... 3 samples from each individual were examined under a bright field microscope (DMI6000B, Leica Microsystems) and representative photomicrographs were taken with a digital camera (DFC310 FX Leica) ...
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bioRxiv - Plant Biology 2020Quote: ... an image of the 3 gynoecia using a stereo microscope (Leica M-205, Leica microsystems) was captured ...
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bioRxiv - Plant Biology 2020Quote: ... an image of the 3 gynoecia using a stereo microscope (Leica M-205, Leica microsystems) was captured ...
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bioRxiv - Cell Biology 2019Quote: ... at least 3 adult worms for each condition were fixed using high pressure freezing (Leica EM PACT2 ...
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections (3 µm thickness) were obtained using a microtome (HM 335E; Leica Microsystems) and stained with 0.2% toluidine blue O for observation under a BX51 light microscope (OLYMPUS) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... About 3-4 µm sections were prepared using an automated microtome (RM2255, Leica Biosystems, Germany) and the sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2023Quote: ... TA muscles were embedded in 3% agarose in PBS and sectioned on a vibratome (Leica) at 100 μm or 200 μm ...
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bioRxiv - Bioengineering 2023Quote: SNO were embedded in 3% low melting agarose and sectioned using Leica VT1000S vibratome (Leica) into slices of 400 μm diameter ...
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bioRxiv - Microbiology 2023Quote: ... covered with the flat side of a 3-mm type-B aluminum platelet (Leica Microsystems). The sample was vitrified by high-pressure freezing using an HPM100 system (Leica Microsystems ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Neuroscience 2021Quote: ... Cells were imaged with a LEICA DMLFSA microscope fitted with a 63x water-immersion objective (HCX APO L 63x/0.9W U-V-I, Leica) and a LEICA TCS SP2 confocal scan head ...
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bioRxiv - Neuroscience 2021Quote: ... Live imaging was performed using a Leica SP5 confocal system equipped with an acousto-optical beam splitter and an HC Fluotar L 25X/0.95 W VISR immersion objective (Leica). Fluorescence was excited by a 405 nm laser and collected by two photomultiplier tubes set to 440-500nm (mTurquoise2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Fluorescent images were obtained using a fluorescence microscope (LAS AF with DMI 6000B-AFC) with HCX PL FLUOTAR L 40×/0.60na CORR PH2 objective lens (Leica). Acquired images were pseudo colored and merged using the ImageJ software ...
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bioRxiv - Immunology 2019Quote: ... Standard confocal microscopy was performed using a Leica TCS SP2 equipped with a Leica HCX APO L 40.0×0.80 W UV water objective (Leica microsystems) at a voxel resolution of 0.36 × 0.36 × 1 µm.
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bioRxiv - Biophysics 2022Quote: ... where it was focused by a cylindrical lens into the back-focal plane of a water-dipping objective (40× 0.8 NA HCX Apo L W, Leica) and subsequently reflected by the chip of an AFM cantilever (custom-coated cantilever based on model ...
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bioRxiv - Neuroscience 2022Quote: ... Time-lapse recordings were performed using a Leica SP5 or SP8 inverted confocal microscope with a 20× long-operation objective lens (HC PL FLUOTAR, L 20x/0.40 CORR, Leica). The maximum intensity projection was generated from 10-15 Z-stack images with 10-μm intervals at each time point.
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bioRxiv - Neuroscience 2024Quote: ... under isoflurane anesthesia (2.5%, 15.l/min) male mice were impacted over the left somatosensory cortex using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Developmental Biology 2023Quote: All imaging was performed on a white-light Leica TCS SP8 inverted confocal microscope using either an HC FLUOTAR L 25×/0.95 VISIR water objective (Leica) or an HC PL APO 63x/1.40 CS2 oil objective (Leica) ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Cell Biology 2023Quote: ... was used to acquire high-resolution images to analyse the fluorescent vesicles in the proximal tubules by using 25/1.0 NA water immersion objective (HC IRAPO L, Leica). The excitation wavelength was 1040 nm tunable laser for imaging of mCherry ...
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bioRxiv - Biophysics 2023Quote: ... fluorescence and confocal reflection mode using a water dip-in objective (HCX APO L 20x/1.00 W, Leica, Wetzlar). Organoid-generated forces are relaxed after ∼ 24 hours using 10 µM cytochalsin D (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The light sheet is created in the tower by a cylindrical lens that is focused in the back-focal plane of a vertically mounted water-dipping objective (40× 0.8 NA HCX Apo L W, Leica). The tower was placed above the imaging dish and reflected the excitation light sheet laterally with a custom-coated cantilever (40 nm Al coating on both sides of HYDRA2R-100N-TL-20 ...
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bioRxiv - Biophysics 2023Quote: ... Stellaris 5 up-right system using either a HC APO L U-V-I 10x/0.30 NA WATER (Leica) or a HC FLUOTAR L VISIR 25X/0.95 NA WATER (Leica ...
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bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using either p53 polyclonal antibodies (Leica Biosystems, Cat # NCL-L-p53-CM5p) or Mettl3 polyclonal antibodies (Abclonal ...
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bioRxiv - Genetics 2020Quote: ... The cell suspension was spread on a poly-L-lysin-coated slide before observing by Structured Illumination Microscopy (SIM) with 100X/1.4 NA STED White Objective (Leica Microsystems) and VT-iSIM system (Visitech International) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Images were acquired with a Leica SP8 confocal microscope using a HCX IRAPO L 25X/0,95NA water immersion objective (#11506340, Leica microsystems). Ventricule volume is estimated by the simplified ellipsoid volume calculation formula V=0.523(width in mm)2(length in mm ...
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bioRxiv - Neuroscience 2019Quote: ... The laser beams are focused into the tissue by an HCX IRAPO L water immersion objective (25x/0.95 W CORR; Leica Microsystems). The forward-detected CARS signal is measured via a non-descanned detector ...
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bioRxiv - Pathology 2020Quote: ... Lungs biopsies were acquired with Leica SP8 confocal/two-photon microscope using a HCX IRAPO L 25X/0,95NA water immersion objective (Leica microsystems). For identification of RSV infected cells ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence was detected through the objective (Olympus LUMPLFLN 60XW, 1.0 NA, or Leica HC FLUOTAR L 25x/0.95 W VISIR) and through the oil immersion condenser (numerical aperture 1.4 ...
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bioRxiv - Neuroscience 2022Quote: Immunostained samples were acquired by confocal and 2-photon microscopy using the same Leica SP8 and a coverslip corrected HCX IRAPO L 25X/0.95NA water immersion objective (Leica Microsystems). Alexa Fluor 488 and Alexa Fluor 594 were excited with lasers emitting at 488 nm and 552 nm wavelengths respectively ...
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bioRxiv - Biochemistry 2024Quote: Droplet fusion was recorded in mixtures containing 20 µM Loc1p and 0.5x mass ratio total HeLa RNA with the above-mentioned setup (HC PL Fluotar L 40x/0.6 PH2 objective on Leica DMi8) in movie mode ...