Labshake search
Citations for Leica :
801 - 850 of 870 citations for L Tyrosine Ring 13C6 99%; 3 3 D2 30% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... the samples were dehydrated in a graded series of ethanol (30-100%) and embedded in EmBed 812 using an automated microwave tissue processor for electronic microscopy (Leica EM AMW). Ultrathin sections (70 nm ...
-
bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
-
bioRxiv - Microbiology 2023Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a pig experimentally infected with swine influenza virus A/swine/Texas/4199-2/1998 H3N2 were used as a positive assay control.
-
bioRxiv - Neuroscience 2023Quote: ... 30 μm) embedded in TissueTek (Sakura Finetek, California, USA) and sectioned at -20°C on a Leica CM1950 cryostat (Leica, Wetzlar, Germany).
-
bioRxiv - Neuroscience 2024Quote: ... Tilted at 30° horizontal cortico-striatal slices or horizontal slices containing SNpc were prepared on a vibratome (Leica VT 1200S, Leica Biosystems) in ice-cold choline chloride-based cutting solution of the following composition (mM) ...
-
bioRxiv - Biophysics 2024Quote: ... 4 μL of 1 mg/mL of proteoliposomes were deposited during 30 s on the grid before flash frozen in liquid ethane with an EM-GP plunger (Leica Microsystems, Germany). Images were taken under low dose condition on a Glacios electron microscope (Thermofisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue clearing was performed using the FluoClearBABB approach24 and whole mount images were then acquired using a SP8 microscope equipped with a white light laser and a HCX APO L 20x/0.95 IMM lens (Leica Microsystems, Germany). Imaging data was analyzed on a workstation (Thinkmate ...
-
bioRxiv - Immunology 2021Quote: ... Six consecutive staining cycles were performed using the following 1ry Antibody-Opal fluorophore pairings: CD4 (clone 4B12, NCL-L-CD4-368 (Leica Novocastra) – Opal 520) ...
-
bioRxiv - Neuroscience 2022Quote: ... osmolarity 280-320 mOsm/l) was used both to keep tissue cold during dissection and as the bathing solution during vibratome slicing (Leica VT1200S). 0.4-1 mm-thick dorsal OB slices were taken by near-horizontal slicing ...
-
bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
-
bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 2% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was locally anesthetized with 40– 50 μL lidocaine 0.5% and disinfected with betadine.
-
bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
-
bioRxiv - Neuroscience 2020Quote: ... Following overnight storage in 4% formalin, brains were stored in 30% sucrose until fully saturated, then sectioned via vibratome (Vibratome, Model 1000plus) or cryostat (Leica Microsystems, Model CM3050S). Viral expression and implant placements were imaged on a standard epifluorescent microscope (Olympus ...
-
bioRxiv - Neuroscience 2019Quote: ... Brains were cut into four or five series of 30 μm-thick coronal-plane sections using a freezing microtome (Leica SM2000R, Nussloch, Germany) and stored in an antifreeze solution containing 50% formalin ...
-
bioRxiv - Cell Biology 2019Quote: The gastrocnemius and quadriceps muscles were isolated from mouse hind limbs and dehydrated in a 30% sucrose solution and then sectioned in a constant cold section box (Leica CM 3000, Germany) at −20 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Cryosections were prepared from tissue embedded in a 2:1 mixture of 30% sucrose: OCT (Sakura Finetek, catalog #4583) onto X-tra slides (Leica Microsystems, catalog #3800200). Tissue sections were dried ...
-
bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
-
bioRxiv - Biophysics 2020Quote: ... The plugs were then sliced perpendicular to the articular surface in 30 μm-thick sections by a vibratome (VT-1000S, Leica Microsystems Inc., Germany) in physiological buffer to preserve cell viability ...
-
bioRxiv - Cell Biology 2021Quote: ... Lacey carbon film grids (EM Resolutions) were glow discharged for 30 sec in a Leica ACE 600 Coater system (Leica Microsystems, Heidelberg, Germany). 5 ul of sample were pipetted onto the grids and the sample plunge frozen in liquid ethane using a Leica GP Plunge freezing device (Leica Microsystems ...
-
bioRxiv - Microbiology 2022Quote: ... and approximately 3.5 µL of purified virus particles were applied to freshly glow-discharged (10 mA for 30 sec using a Leica Ace600 glow-discharger) Quantifoil R2/1 300-mesh holey carbon-coated copper grids ...
-
bioRxiv - Genetics 2022Quote: ... Light was delivered in pulses of 30s of blue LED (from the LED light source of the inverted laboratory microscope LEICA DM IL LED) at 1 Hz (Table S3) ...
-
bioRxiv - Plant Biology 2021Quote: ... The root segments were embedded in agarose 5% (w/v) and cut into 25-30 μm radial sections with a VT1200S vibratome (Leica Microsystems, Nanterre, France). Radial sections were sampled from 15 ectomycorrhizal rootlets (at 200 µm from the root apex ...
-
bioRxiv - Cell Biology 2019Quote: ... the cells were stained with 1 ml oil red O working solution (catalogue No. S0131, Cyagen Biosciences) for 30 minutes and visualized with a light microscope (Leica DMIRB, Heidelberg, Germany). The dye from oil red O staining was extracted using isopropanol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
-
Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... at 1:1500 dilution for 30 minutes followed by detection with horseradish peroxidase (HRP)-conjugated polymer system (Bond Polymer Refine Detection Kit, Leica, Concord, ON, Canada). All sections were then stained using 3,3’-Diaminobenzidine (DAB ...
-
bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
-
bioRxiv - Genetics 2020Quote: ... Cell images were taken 30 min after the ligand treatment using a confocal laser scanning microscope (Leica TCS-SP8; Leica microsystems, Wetzlar, Germany).
-
bioRxiv - Molecular Biology 2022Quote: Wound healing response at day 7 and scar tissue morphology and ECM architecture at day 30 post-wounding were determined by hematoxylin and eosin (H&E) staining and Masson’s Trichrome Staining respectively (Leica Biosystems, Buffalo Grove, IL). Epithelial gap ...
-
bioRxiv - Microbiology 2022Quote: ... back-blotted for 6-8 s after a 30-s pre-blotting time and immediately plunge frozen into liquid ethane using a GP-EM2 (Leica Microsystems, Wetzlar, Germany) with a chamber maintained at 20 °C and 95% humidity ...
-
bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
-
bioRxiv - Neuroscience 2022Quote: ... Samples were dried at the critical point (BALTEC CPD 30) and glued on a pin and sputter coated with 10 nm of metal platinum (LEICA EM SCD 500). Micrographs were taken with a scanning electron microscope FEI Quanta FEG 250 (FEI ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
-
bioRxiv - Neuroscience 2023Quote: ... The brainstem areas containing the DVC and BAT sections were put in 15% followed by 30% sucrose until saturated and frozen in a cryo-embedding medium (Leica Biosystems, Nussloch, Germany). Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems ...
-
bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950, Leica Biosystems, Wetzlar, Germany) for a total duration of 1 hour ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains destined to NanoSIMS analysis were processed as before but coronal sections of 30 µm thickness were obtained with a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany) and stored at −20°C in 25% ethylene glycol and 25% glycerol in PBS.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... frozen tissues were longitudinally cut into 30-μm thick (spinal cord) or 12-μm thick (DRG and SCN) sections using a cryostat (Leica Microsystems, Wetzlar, Germany) and floated in PBS (spinal cord ...
-
bioRxiv - Plant Biology 2023Quote: ... Transverse sections were cut to a thickness of 30 μm (roots) using a 35A stainless-steel blade (Feather, Osaka, Japan) attached to a cryomicrotome (Leica 3050S, Wetzlar, Germany) at –20 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... The excitation beam was focused with a water-dipping objective lens (Nikon CFI75 16× 0.8NA or Leica HC FLUOTAR L 25x 0.95NA). The average power was 100-150 mW at the sample ...
-
bioRxiv - Developmental Biology 2019Quote: ... samples were mounted for imaging on a Leica SP8 confocal with a dipping immersion objective (Leica HCX IRAPO L, 25X, N.A. 0.95). Z-stacks were taken every 10 µm ...
-
bioRxiv - Plant Biology 2020Quote: ... containing 1 g/l calcofluor white M2R and 0.5 g/l evans blue and then mounted on a TCS SP5 II CLSM (Leica Microsystems, Vienna, Austria). As emission source a 405 nm UV diode was used ...
-
bioRxiv - Biochemistry 2021Quote: ... 4 μL sample were applied onto a grid held in the sample grid chamber of a Leica EM GP instrument (Leica Microsystem Inc.) cooled at 4 °C ...
-
bioRxiv - Plant Biology 2023Quote: ... T1 seeds from plants transformed with pSS01_35Spro:LIP1-GFP and pB7WG2_35Spro:LIP1 were harvested and selected either with 240 mg/L glufosinate ammonium solution (Basta) or screened for fluorescence on a stereomicroscope (Leica M165 FC) equipped with a GFP filter (excitation ...
-
bioRxiv - Neuroscience 2019Quote: ... Post-fixed brains were cryoprotected in 30% sucrose and coronal brain sections (50 μm thick) were obtained using freezing microtome (Leica SM200R; Leica, Wetzlar, Germany). Sections were mounted in ProLong® Gold (Invitrogen ...
-
bioRxiv - Bioengineering 2020Quote: ... Samples were attached to SEM stubs using double-sided carbon tape and coated with Au/Pd for 30 seconds using an EM ACE200 sputtercoater (Leica, Buffalo Grove, IL, USA). Images were collected with a JSM-6010LA SEM (JEOL ...
-
bioRxiv - Plant Biology 2021Quote: ... 30 nm of platinum was then sputter coated on the block using a Leica EM SCD 500 sputter coater (Leica Mikrosysteme GmbH, Vienna, Austria). Serial block face imaging is finally performed in a Helios NanoLab 650 (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... the brain of each mouse extending from anterior to the DG to the cerebellum (from 0.22 to −5.34 μm from Bregma) was sectioned at 30 μm in a 1:9 series using a freezing microtome (Leica SM 2000 R Sliding Microtome). Sections were stored in 1xPBS with 0.01% sodium azide at 4°C until processing for IHC ...
-
bioRxiv - Molecular Biology 2021Quote: ... and after several washes in phosphate-buffered saline (PBS) and cryo-preservation in 30% sucrose in PBS were embedded in cryomedium (Surgipath Cryogel®, Leica Microsystems, Vienna, Austria). Subsequently ...
-
bioRxiv - Microbiology 2019Quote: ... each for 30 min at room temperature and 100% for 2 × 1 h and 1 × 30 min) and dried in a Leica EM CPD030 (Leica Microsystems Belgium, Diegem, Belgium). The membranes were mounted on a stub and coated with 5 nm of carbon (Leica Microsystems Belgium ...