Labshake search
Citations for Leica :
751 - 800 of 870 citations for L Tyrosine Ring 13C6 99%; 3 3 D2 30% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... on dry ice and stored at −20 ºC until sectioned into 30 μm coronal slices on a CM1860 Leica cryostat (Leica Microsystems). Slices were mounted onto Colorfrost Plus slides (Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Tilted at 30° horizontal cortico-striatal slices or horizontal slices containing SNpc were prepared on a vibratome (Leica VT 1200S, Leica Biosystems) in ice-cold choline chloride-based cutting solution of the following composition (mM) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Coronal brain sections and transverse spinal cord sections were collected at a 30 μm thickness using a cryostat (Leica, Wetzlar, Germany). Brain slices were blocked with 5% donkey serum in 0.3% PBS-T for 1 h at RT and then incubated with a sheep anti-GFRAL antibody (1:500 ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
-
bioRxiv - Cancer Biology 2024Quote: Time-lapse imaging of live cells was done for 25 hours with a time interval of 30 min between time points on a fully automated DVCore microscope (Leica-microsystems), using a 60x oil objective (N.A ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using either p53 polyclonal antibodies (Leica Biosystems, Cat # NCL-L-p53-CM5p) or Mettl3 polyclonal antibodies (Abclonal ...
-
bioRxiv - Genetics 2020Quote: ... The cell suspension was spread on a poly-L-lysin-coated slide before observing by Structured Illumination Microscopy (SIM) with 100X/1.4 NA STED White Objective (Leica Microsystems) and VT-iSIM system (Visitech International) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen ...
-
bioRxiv - Developmental Biology 2022Quote: ... Images were acquired with a Leica SP8 confocal microscope using a HCX IRAPO L 25X/0,95NA water immersion objective (#11506340, Leica microsystems). Ventricule volume is estimated by the simplified ellipsoid volume calculation formula V=0.523(width in mm)2(length in mm ...
-
bioRxiv - Neuroscience 2019Quote: ... The laser beams are focused into the tissue by an HCX IRAPO L water immersion objective (25x/0.95 W CORR; Leica Microsystems). The forward-detected CARS signal is measured via a non-descanned detector ...
-
bioRxiv - Pathology 2020Quote: ... Lungs biopsies were acquired with Leica SP8 confocal/two-photon microscope using a HCX IRAPO L 25X/0,95NA water immersion objective (Leica microsystems). For identification of RSV infected cells ...
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescence was detected through the objective (Olympus LUMPLFLN 60XW, 1.0 NA, or Leica HC FLUOTAR L 25x/0.95 W VISIR) and through the oil immersion condenser (numerical aperture 1.4 ...
-
bioRxiv - Neuroscience 2022Quote: Immunostained samples were acquired by confocal and 2-photon microscopy using the same Leica SP8 and a coverslip corrected HCX IRAPO L 25X/0.95NA water immersion objective (Leica Microsystems). Alexa Fluor 488 and Alexa Fluor 594 were excited with lasers emitting at 488 nm and 552 nm wavelengths respectively ...
-
bioRxiv - Biophysics 2023Quote: ... around individual cells are recorded in bright-field and confocal reflection mode using a 20x water dip-in objective (HCX APO L 20x/1.00 W, Leica, Wetzlar). The acquisition time for an individual image stack is below 10 seconds ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5µm thick sections were cut using a Leica RM2125 RTS microtome and sectioned onto Poly-L-lysine coated slides (Leica Surgipath Apex Superior adhesive slides ...
-
bioRxiv - Microbiology 2023Quote: ... islandicus REY15A grown under poor media (4.5 μl) was applied to glow-discharged lacey carbon grids and then plunge-frozen using an EM GP Plunge Freezer (Leica). The cryo-EMs were collected on a 300 keV Titan Krios with a K3 camera at 1.08 Å per pixel and a total dose of 50 e-/Å2 ...
-
bioRxiv - Plant Biology 2023Quote: ... Each 3 µl of protein reaction mix was loaded into a flow-chamber and immediately observed with a confocal microscope SP5 (Leica) equipped with a 60x oil objective (NA 1.42) ...
-
bioRxiv - Developmental Biology 2023Quote: Live samples were imaged using a Leica Stellaris 5 laser-scanning confocal microscope with a water HC FLUOTAR L VISIR 25X/0.95 objective and HyD S detector (Leica Microsystems). Larvae were dissected and pinned to expose ventral muscles and imaged live in ambient temperature while the larvae were maintained in ice-cold HL3.1 buffer ...
-
bioRxiv - Microbiology 2023Quote: ... a small volume (5 μL) of the stained suspension is placed on a glass slide and examined under a confocal laser scanning microscope (Leica SP-5 Confocal Laser Scanning Microscope) ...
-
bioRxiv - Microbiology 2024Quote: The flagellar filament sample (4.5 μl) was applied to glow-discharged lacey carbon grids and then plunge-frozen using an EM GP2 Plunge Freezer (Leica). The cryo-EM micrographs were collected on a 300 keV Titan Krios with a K3 camera at 1.11 Å per pixel and a total dose of 50 e-/Å2 ...
-
bioRxiv - Physiology 2024Quote: ... Imaging was performed with a Leica SP8 confocal microscope with an HC APO L 20x/1,00 W objective and LAS X software (Leica Microsystems). The tissue was bath perfused with bubbled room-temperature Krebs buffer (118 mM NaCl ...
-
bioRxiv - Biochemistry 2024Quote: Droplet fusion was recorded in mixtures containing 20 µM Loc1p and 0.5x mass ratio total HeLa RNA with the above-mentioned setup (HC PL Fluotar L 40x/0.6 PH2 objective on Leica DMi8) in movie mode ...
-
bioRxiv - Biochemistry 2024Quote: ... Condensates were imaged by phase contrast microscopy using an HC PL Fluotar L 40x/0.6 PH2 objective on a Leica DMi8 (Leica, Germany). Experiments were performed at least three independent times.
-
bioRxiv - Cancer Biology 2021Quote: Unstained sections from the study cases were heated for 30 minutes in Bond™ Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems) using Leica BOND-MAXTM (Leica Microsystems) ...
-
bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
-
bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
-
bioRxiv - Neuroscience 2022Quote: ... The tissue was then sectioned transversally at 30 μm by means of a cryostat (Leica CM3050 S, Leica Biosystems AG, Muttenz, Switzerland) and placed on microscope slides to dry for at least one hour ...
-
bioRxiv - Neuroscience 2021Quote: ... Each volumetric scan consisted of five B-scans (1000 A-scans per B-scan by 30 frames) which were co-registered and averaged using InVvoVue software (Leica Microsystems Inc.). Each B-scan was ∼1.8 mm in width and had an axial resolution of 1.4 µm.
-
bioRxiv - Neuroscience 2019Quote: ... Post-fixed brains were cryoprotected in 30% sucrose and coronal brain sections (50 μm thick) were obtained using freezing microtome (Leica SM200R; Leica, Wetzlar, Germany). Sections were mounted in ProLong® Gold (Invitrogen ...
-
bioRxiv - Plant Biology 2019Quote: ... was performed at 30 days after watering as described in (Hiss et al. 2017) using a Leica S8Apo binocular (Leica, Wetzlar, Germany). Counting of the crosses was performed according to (Perroud et al ...
-
bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissues were embedded in Optimal Cutting Temperature compound (OCT, Sakura) and sectioned into 30-μm slices using a cryostat (Leica CM3050 S). Frozen tissues stored at −80°C ...
-
bioRxiv - Neuroscience 2022Quote: ... the perfused and postfixed brains were equilibrated in RNase-free 30% sucrose and then cut into 40-μm-thick coronal sections on a cryotome (Leica CM300, Leica). Floating brain sections were placed in chamber slides (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... frozen human brains were sliced at a temperature of −30 °C into 60 μm-deep coronal sections using a cryostat microtome (Leica Microsystems, Germany). Finally ...
-
bioRxiv - Neuroscience 2020Quote: Whole brains (fixed) were sliced in coronal sections (30 μm thickness) on a sliding freezing microtome (Leica CM1900, Leica Microsystems, Wetzlar, Germany) as previously described (Lundquist ...
-
bioRxiv - Neuroscience 2019Quote: ... Brains were embedded in 6% agarose and sectioned coronally at 30-micron thickness using a Leica VT1000S vibratome (Leica Camera, Wetzlar, Germany).
-
bioRxiv - Neuroscience 2019Quote: ... Brains were then immersed in PBS solution with additional 30% sucrose for 24h and then cut in a microtome (80 μm thick, SM 2000R; Leica, Heidelberg, Germany).
-
bioRxiv - Neuroscience 2022Quote: ... Tissues were covered with Optimal Cutting Temperature (OCT) medium (CellPath, Newtown, UK) and sections were obtained at 30 μm on a Leica SM2010R Freezing Microtome (Leica, Wetzlar, Germany). All sections were subjected to heat-mediated antigen retrieval in 10 mM sodium citrate (pH6.5 ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... The brains were then transferred to a 30% sucrose solution and finally sectioned coronally (40 μm) using a cryostat (Leica Biosystems, Switzerland). The sectioned slices were immediately immersed in cryoprotectant solution and stored at −20°C.
-
bioRxiv - Physiology 2022Quote: ... Samples were embedded in the Optimal Cutting Temperature compound and sectioned to 20–30 µm at 50 °C using a cryostat (Leica CM 3050S) and stained with haematoxylin & eosin using Sakuras Tissue-Tek DRS 2000 at the Department of Anatomy ...
-
bioRxiv - Pathology 2022Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a SARS-CoV-2-infected hamster were used as positive assay controls.
-
bioRxiv - Neuroscience 2023Quote: ... Serial sections were collected onto cover glasses and coated with gold with sputter current 30 mA for 5 seconds (Leica EM ACE200). Cover glasses were sealed on aluminum stabs using carbon tape ...
-
bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
-
bioRxiv - Neuroscience 2023Quote: ... Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems, Nussloch, Germany). Brain sections were labelled with Anti-FLAG M2 F1804 ...
-
bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation at 1:250 dilution for 30 minutes was followed by detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.