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Citations for Leica :
651 - 700 of 870 citations for L Tyrosine Ring 13C6 99%; 3 3 D2 30% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Biochemistry 2021Quote: ... Lenses were sectioned to 30 μm thickness using a CM 3050 Cryostat (LEICA CM 3050S, Leica Microsystems Inc., Bannockburn, IL). The sections were transferred to glass slides and washed three times in PBS before mounting on glass slides with mounting media (Vector Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Genomics 2021Quote: ... USA) and 30 µm sections cut into phosphate-buffered saline (pH 7.4) using a vibratome (Leica VT1000S Vibratome, Wetzlar, Germany). The sections were examined using bright field microscopy on a BX53 microscope fitted with a UPlanfl N 20x/0.5 objective and coupled to a SC180 camera with cellSens standard software (v 2.3 ...
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bioRxiv - Neuroscience 2020Quote: ... Sample sections (15 μm in thickness for IHC or 30 μm thickness for Sholl analysis) were prepared on gelatin-coated glass slides using a cryostat (Leica). The sections were blocked with 5% goat serum and 0.3% triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... the perfused and postfixed brains were equilibrated in RNase-free 30% sucrose and then cut into 40-μm-thick coronal sections on a cryotome (Leica CM300, Leica). Floating brain sections were placed in chamber slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cryoprotected with 30% sucrose in 0.1 M phosphate buffer to prepare 50 μm-thick cryosections on a cryostat (CM1900; Leica Microsystems). All immunohistochemical incubations were performed at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... The injury section was further cut into 30 μm sections and mounted onto a series of 10 slides coated with Silane Surgipath Solution (Leica). These slides were then stored at −80° C ...
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bioRxiv - Neuroscience 2019Quote: ... The OE were embedded in 4% low melting point agarose blocks and sectioned (30 µm) using a Vibratome (VT 1200; Leica). The sections were blocked in 1% blocking solution (10 min at RT ...
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bioRxiv - Pathology 2019Quote: ... RTU (Vector ImmPress) for 30 min after which the brown color was developed with peroxidase block and Novolink DAB (Leica). A 1:3000 dilution of rabbit anti-CD163 antibody for 15 min and then anti-rabbit Polymer AP (Leica ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IHC at a 1:50 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine RED Kit (Leica) minus Hematoxylin.
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bioRxiv - Neuroscience 2021Quote: ... Coronal sections (30 μm thickness) were collected from within and around the scanning FOV site using a cryostat (Leica Biosystems). Brain sections were permeabilized using 0.2% Triton-X100/PBS (PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with either 10 μM DHE or 5 μM MitoSOX for 30 min at 37°C in the dark and analysed by flow cytometry or in a DMi8 fluorescence microscope (Leica).
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bioRxiv - Neuroscience 2021Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Neuroscience 2020Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating sections with a vibrating microtome (Leica). Brain sections were kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was frozen using dry ice and coronally sectioned in 30 µm thickness on a microtome (Leica SM2010R, Germany). One out of every three sections was mounted for examination of virally labeled neurons in different brain structures ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were incubated for 30 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S ...
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired every 15 min at 30°C for 17 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL 614 APO 63x/1.4 Oil ph3 objective Leica) ...
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bioRxiv - Plant Biology 2022Quote: ... roots were dipped in propidium iodide (PI) solution (10 µg/ml) for 30 secs to stain the root cell wall (Leica SP8 confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: ... and brains were postfixed in 4% paraformaldehyde and preserved with 30% sucrose before sectioning at 40-µm on a freezing-sliding microtome (SM2010R, Leica). Sections were incubated overnight with indicated primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in PFA overnight at 4 °C and dehydrated in 30% sucrose (in PBS) for at least 18 hours before vibratome sectioning (VT1000s, Leica). Brains were cut into 50 µm coronal section using cryostat (CM 1950 ...
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bioRxiv - Neuroscience 2022Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (25 μm-thick sections) using a Leica Cryostat (Leica, CM1860). For immunofluorescence staining ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse brains were frozen and sectioned sagittally with a thickness of 30 µm on a cryostat (Leica Biosystems, Germany), then the tissue slides were air dried for next step ...
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bioRxiv - Neuroscience 2022Quote: ... Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v) sucrose for two days and then embedded in tissue freezing medium (Leica Microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... Spheroids were incubated in 30% sucrose overnight and imbedded in Tissue-Tek OTC (Sakura) for cryosectioning at 40μm slices on a cryostat (Leica CM3050s). Fixed cells or slide-mounted spheroid slices were incubated with gentle agitation for 1 hour in blocking buffer (5% BSA ...
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bioRxiv - Neuroscience 2023Quote: Ambra1 WT and Het female mouse brain were cut into coronal sections (30 μm) with Leica CM1950 instrument (Leica Biosystems). Sections were blocked with 10% normal horse serum (NHS ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was embedded in OCT (SAKURA, 4583) and sectioned coronally (30 μm thick) with a freezing microtome (Leica CM1950). For immunofluorescent staining ...
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bioRxiv - Plant Biology 2023Quote: ... These were dehydrated with successive gradients of ethanol (30-100%) and permeated with paraplast to cut 8-12μm sections (using Leica Microtome). The human fat biopsy tissue and potato tubers were processed similarly ...
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bioRxiv - Biophysics 2023Quote: ... A volume of 3ul of the sample was applied onto glow-discharged (Leica EM ACE200, 30 s at 5 mA) grids (Quantifoil R1.2/1.3 200 mesh Cu grids ...
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bioRxiv - Neuroscience 2023Quote: ... brains tissue fixed in 4% paraformaldehyde were cryoprotected in 30% sucrose for 2 hours followed by embedding in OCT for sectioning to 10μm thickness on a cryostat (Leica CM1860). Prior to staining ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.1M phosphate buffer (PB) for 30-40 min and PFC containing sections of 50-100 μm thickness were prepared using a Vibratome (Leica) and stored in 0.1M PB with 0.05% Na-azide until further processing.
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded and mounted with Tissue-Tek OCT compound (Sakura finetek) and 30 μm sections were cut using a cryostat (Leica). For P2Y12 staining ...
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bioRxiv - Neuroscience 2023Quote: ... Frozen sections of the spinal cord were prepared in the horizontal plane at 30 μm thickness using a cryostat microtome (Leica) and processed for immunofluorescence as described26.
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bioRxiv - Neuroscience 2023Quote: ... Dorsal striatum and/or midbrain coronal slices of 30 µm thickness were obtained using a Leica CM1950 cryostat (Leica Microsystems).
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Molecular Biology 2023Quote: ... acquiring a volume stack of 30 µm thickness with a Z-spacing of 300 nm using the LAS X Navigator software (Leica). Image stacks were deconvolved with AutoQuant X3 (Media Cybernetics ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were de-hydrated in 30% sucrose and sliced in 20 μm coronal cryosections using a CM 3050S cryostat (Leica). Slices were permeabilized and blocked in 1x Phosphate-Buffered Saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... or FM464 (5 µg/mL) dyes for 30 min and imaged by phase-contrast and fluorescence using an inverted DeltaVision Elite Microscope (Leica) equipped with an UPLFLN100XO2/PH3/1.30 objective (Olympus) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections of 30 µM thickness and 600 µM apart were sectioned using a Leica cryostat (Leica Biosystems, Wetzlar, Germany), starting at -0.5 anterior-posterior (AP ...
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bioRxiv - Neuroscience 2023Quote: ... we sectioned half-brains at 25 µm thickness on frozen 30% sucrose solution slabs on a sliding microtome (Leica SM2000R) equipped with freezing stage ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...
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bioRxiv - Plant Biology 2024Quote: ... The specimen carriers were then rapidly frozen (within 30 msec at a pressure of 2,100 Pa) using a Leica EM ICE High Pressure Freezer (Leica Microsystems). Following freezing ...
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bioRxiv - Cell Biology 2023Quote: ... The brains were embedded in OCT tissue TEC (Sakura) and 30 µm-thick frozen coronal sections were cut using a model CM 3050 cryostat (Leica). The sections were blocked with blocking buffer for 1 h and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The embedded brains were frozen in a bath of isopentane at −30°C and stored at −80°C until sectioning using a cryostat (Leica). Immunostainings were performed following standard protocols ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed brains of WT and trappc9 KO mice were cut into 30 μm thick coronal sections on a cryostat (Leica). Sections were placed in cryoprotectant solutions containing 30% ethylene glycol and 30% glycerol prepared in PBS and stored at -20°C until use.
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections (30 μm) were cut from the anterior part of the brain to the midbrain using a cryostat (Leica) at -20°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and then incubated in succession in 20% and 30% sucrose solution for at least 24 hours each before being embedded in Tissue freezing medium (Leica). Histological and immuno-fluorescence/histochemistry experiments were carried out on 6 μm sections ...