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Citations for Leica :
701 - 750 of 870 citations for L Tyrosine Ring 13C6 99%; 3 3 D2 30% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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bioRxiv - Neuroscience 2021Quote: ... Cells were imaged with a LEICA DMLFSA microscope fitted with a 63x water-immersion objective (HCX APO L 63x/0.9W U-V-I, Leica) and a LEICA TCS SP2 confocal scan head ...
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bioRxiv - Neuroscience 2021Quote: ... Live imaging was performed using a Leica SP5 confocal system equipped with an acousto-optical beam splitter and an HC Fluotar L 25X/0.95 W VISR immersion objective (Leica). Fluorescence was excited by a 405 nm laser and collected by two photomultiplier tubes set to 440-500nm (mTurquoise2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Fluorescent images were obtained using a fluorescence microscope (LAS AF with DMI 6000B-AFC) with HCX PL FLUOTAR L 40×/0.60na CORR PH2 objective lens (Leica). Acquired images were pseudo colored and merged using the ImageJ software ...
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bioRxiv - Immunology 2019Quote: ... Standard confocal microscopy was performed using a Leica TCS SP2 equipped with a Leica HCX APO L 40.0×0.80 W UV water objective (Leica microsystems) at a voxel resolution of 0.36 × 0.36 × 1 µm.
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bioRxiv - Biophysics 2022Quote: ... where it was focused by a cylindrical lens into the back-focal plane of a water-dipping objective (40× 0.8 NA HCX Apo L W, Leica) and subsequently reflected by the chip of an AFM cantilever (custom-coated cantilever based on model ...
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bioRxiv - Neuroscience 2022Quote: ... Time-lapse recordings were performed using a Leica SP5 or SP8 inverted confocal microscope with a 20× long-operation objective lens (HC PL FLUOTAR, L 20x/0.40 CORR, Leica). The maximum intensity projection was generated from 10-15 Z-stack images with 10-μm intervals at each time point.
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Cell Biology 2023Quote: ... was used to acquire high-resolution images to analyse the fluorescent vesicles in the proximal tubules by using 25/1.0 NA water immersion objective (HC IRAPO L, Leica). The excitation wavelength was 1040 nm tunable laser for imaging of mCherry ...
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bioRxiv - Biophysics 2023Quote: ... fluorescence and confocal reflection mode using a water dip-in objective (HCX APO L 20x/1.00 W, Leica, Wetzlar). Organoid-generated forces are relaxed after ∼ 24 hours using 10 µM cytochalsin D (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The light sheet is created in the tower by a cylindrical lens that is focused in the back-focal plane of a vertically mounted water-dipping objective (40× 0.8 NA HCX Apo L W, Leica). The tower was placed above the imaging dish and reflected the excitation light sheet laterally with a custom-coated cantilever (40 nm Al coating on both sides of HYDRA2R-100N-TL-20 ...
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bioRxiv - Biophysics 2023Quote: ... Stellaris 5 up-right system using either a HC APO L U-V-I 10x/0.30 NA WATER (Leica) or a HC FLUOTAR L VISIR 25X/0.95 NA WATER (Leica ...
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bioRxiv - Neuroscience 2024Quote: ... under isoflurane anesthesia (2.5%, 15.l/min) male mice were impacted over the left somatosensory cortex using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Developmental Biology 2023Quote: All imaging was performed on a white-light Leica TCS SP8 inverted confocal microscope using either an HC FLUOTAR L 25×/0.95 VISIR water objective (Leica) or an HC PL APO 63x/1.40 CS2 oil objective (Leica) ...
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bioRxiv - Physiology 2021Quote: ... Heat-induced antigen retrieval was performed with citrate buffer (pH 6) for 30 minutes (AR9961; Bond TM Epitope Retrieval Solution; Leica Biosystems) in 2-μm-thick sections ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were equilibrated in 30% methanol and deposited in a Leica EM AFS2/FSP automatic reagent handling apparatus (Leica Microsystems). Lowicryl polymerization under UV was for 40 h at – 20°C followed by 40 h at +20°C Ultrathin-sections of Lowicryl-embedded material were collected on Formvar-carbon-coated gold grids (200 mesh ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were washed in PTW and incubated once overnight in 30 % sucrose in PTW and once in Tissue Freezing Medium (Leica, #14020108926): 30 % Sucrose in PTW (1:1 ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... Detached nodules were embedded in 5% low melting point agarose and sliced into 30-μm thick sections with a rotary microtome RM2235 (Leica Microsystems). Roots were also stained with X-Gal (Affymetrix ...
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bioRxiv - Genomics 2021Quote: ... primary antibody was diluted 1:10 in the peptide solution directly.) The primary antibodies were detected by 30 minute incubation with HRP-labeled anti-mouse secondary antibody (catalog# PV6114, Leica Microsystems) followed by detection with 3,3′-Diaminobenzidine (catalog# D4293 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Neuroscience 2020Quote: The fixed hemisphere was serially sectioned at 30 µm transverse to the long axis of the hippocampus using a frozen microtome (Leica, Germany). For assessing microglial density and activity ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Neuroscience 2020Quote: ... Sagittal sections (30 μm thickness) were taken around SNc and where the optical fiber was visible on a cryostat (Leica Microsystems). To verify localization of viral expression in dopamine neurons and optical fiber placement above SNc ...
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bioRxiv - Neuroscience 2019Quote: ... snapshots of the worm were taken at 15 frames per second for 30 s using a IMAGINGSOURCE camera (DMK37BUX273) mounted on a stereomicroscope (LEICA M165FC). The worm was constantly kept in the center of the view field with a motorized microscope stage (OptiScanTM ES111 ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse brains were dehydrated in 30% sucrose and sectioned at a thickness of 40 μm with a cryostat (Leica Microsystem), and the brain sections were immunostained for mCherry (1:1000).
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bioRxiv - Neuroscience 2022Quote: ... A total of 36 serial sections containing transverse ventral-medial hippocampus were prepared at 30 μm using a frozen sledge microtome (Leica SM2010R). Sections were collected into 0.02% sodium azide in 0.1 M PBS for immunohistochemistry ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Colonies were randomly selected and imaged 30 min after incubation on a SP8 confocal microscope equipped with an AOBS device (Leica Microsystems).
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bioRxiv - Neuroscience 2022Quote: Perfused brains were cryoprotected with 30% sucrose solution (Fisher Schientific, UK) and sliced at 50 μm using a SM2010R freezing microtome (Leica, UK). The slices were mounted using HardSet Mounting Medium with DAPI (Vector Labs ...
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bioRxiv - Cancer Biology 2022Quote: ... spheres were incubated in n-Butanol (three times 30 min) and melted paraffin (three times) before they were Paraffin-embedded using the HistoCore Arcadia H (Leica Biosystems) following the manufacturer’s protocol in a medium size mold ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were frozen and coronal sections of 25 or 30 μm containing the rostral striatum and midbrain were cut on a cryostat (Leica, Germany). Free-floating midbrain and striatal tissue sections were stained following standard protocols.
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bioRxiv - Neuroscience 2020Quote: ... Fixed embryonic brains were sectioned into 30 μm thick sections in the coronal plane using a Leica Cryostat CM1850 (Leica Microsystems). Sections were permeabilized with 0.3% Triton X100 ...
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bioRxiv - Neuroscience 2020Quote: ... the brain was sliced frozen to 30 μm thick coronal sections using a sliding microtome (Leica SM 2010R; Nussloch, Eisfeld, Germany) into eight wells in a serial manner ...
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bioRxiv - Neuroscience 2021Quote: ... Sakura Finetek) and 30% sucrose–PBS (1:1) for cryosectioning (18-20 μm-thick sections) using a Leica Cryostat (Leica, CM1850). Plated hSS cultures on glass coverslips were fixed in 4% PFA at room temperature for 10 minutes and washed with then rinsed twice for 5 minutes with PBS.
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bioRxiv - Bioengineering 2021Quote: ... Sections were incubated (30 min, room temperature (rt)) with an anti-GFP antibody (see Table S2) in Leica Primary Antibody Diluent (Leica Biosystems). Bond Polymer Refine Detection (Leica Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... The brains were then removed and postfixed in 4 °C overnight and immersed in 30% sucrose solution for 2 days before being sectioned at 50 μm thicknesses on a cryostat (CM3050 S; Leica Biosystems). Imaging was performed with a scanning microscope (BX61VS ...
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bioRxiv - Genetics 2021Quote: ... The fertilized eggs were co-injected with the purified Cas9 enzyme (50 ng/μl) and sgRNA (30 ng/μl) under a Leica inverted microscope equipped with Leitz micromanipulators (Leica Microsystems). Injected eggs were incubated overnight in KSOM-AA medium (Millipore Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen brains were sectioned transversally into 30 μm thick slices at −20°C to −25°C using a Leica CM 3050S cryostat (Leica Microsystems). Floating brain sections were washed with 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: Slices were serially sectioned in the horizontal plane at 30-40μm thick using a Leica CM1950 (Leica Biosystems, Buffalo Grove, Illinois) and collected as free floating sections in PBS in 24 well plates ...
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bioRxiv - Cancer Biology 2020Quote: ... applied at either 1:300 or 1:500 for 15-30 minutes prior to detection and color visualization using Refine DAB Polymer (DS9800, Leica Biosystems). Rabbit primary antibodies were incubated for either 15 or 30 minutes followed by detection with Refine DAB Polymer ...
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bioRxiv - Neuroscience 2022Quote: ... cryoprotected in 0.1M phosphate buffer solution containing 30% Sucrose (w/v) and cut into 50 μm thick transversal sections with a cryostat microtome (CM3050S, Leica Biosystems). Sections were counterstained with the nuclear dye 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... Seminal roots were observed with a fluorescence microscope at 460/40 nm emission and 510/30 nm excitation (DMR HC, Leica, Germany), but due to their relatively large diameter ...
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bioRxiv - Neuroscience 2022Quote: Mouse brains were drop-fixed overnight in 4% PFA in 1X PBS and transferred to a 30% sucrose solution in 1X PBS before slicing (40 or 50 mm) on a sliding microtome (Leica Microsystems). The slices were permeabilized and blocked in 3% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were cryoprotected by incubating >24h in 15% sucrose in PBS followed by 30% sucrose before being frozen and sectioned at 30μm with a cryostat (Leica CM 1950). For stains using primary antibodies raised in mice ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellets were equilibrated in 30% methanol and deposited in a Leica EM AFS2/FSP automatic reagent handling apparatus (Leica Microsystems). Lowicryl polymerization under UV was performed for 48 h at – 20°C ...
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bioRxiv - Bioengineering 2023Quote: ... samples were frozen at -20⍰C and cryosections with 30 μm thickness were prepared Leica CM1950 cryomicrotome (Leica BioSystems, Diegem, Belgium). Slides were then thawed on Superfrost glass slides (Epredia ...
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bioRxiv - Molecular Biology 2024Quote: ... Coronal brain sections and transverse spinal cord sections were collected at a 30 μm thickness using a cryostat (Leica, Wetzlar, Germany). Brain slices were blocked with 5% donkey serum in 0.3% PBS-T for 1 h at RT and then incubated with a sheep anti-GFRAL antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... coronal brain sections (50 μm thickness for ScEM – 30 μm thickness for IF) were generated using a vibratome (Leica Vibratome VT1000S.) and stored at −20°C in cryoprotectant solution (30% glycerol ...
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bioRxiv - Neuroscience 2023Quote: ... they were embedded in OCT compound (Tissue-Tek, Sakura) and transverse sections of 30 μm thickness were cut in a cryostat (Leica cm1950) and collected on gelatinized slides ...
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bioRxiv - Neuroscience 2023Quote: ... After the brain samples were fixed in 4% paraformaldehyde overnight, they were dehydrated in 30% sucrose (Daejung, Korea) and embedded in frozen section compound (Leica, Germany). Coronal sections (10 µm ...