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Citations for Polysciences :
251 - 300 of 811 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Microbiology 2021Quote: ... one half of the ovaries of each of 6 crickets was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, USA) in 100 mM phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 S gene expression vector (wild type or mutant) in conjunction with p8.9138 and pCSFLW39 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Neuroscience 2019Quote: ... for 10 min on 2% agarose pad and then immobilized using 1.5 μl Polystyrene 0.10 micron microspheres and a coverslip (Polysciences, Inc, Cat# 00876). GCaMP3 imaging was performed on a Zeiss AxioImager microscope using a Q-Imaging Rolera EM-C2 EMCCD camera ...
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bioRxiv - Biochemistry 2020Quote: ... washed in two drops of water and stained 30 s in either 0.75% uranyl formate (UF) or 2% sodium phosphotungstate (NaPT, Polysciences Inc. USA), pH 7.5.
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bioRxiv - Immunology 2020Quote: A microsphere suspension was prepared by diluting 2 μm fluorescent polystyrene microspheres (Fluoresbite® YG Carboxylate Microspheres, Polysciences, 2.5% microsphere suspension) 1:1 (v/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed and incubated in serum-free MEM media for 2 h prior to addition of 1.75-micron latex beads (Fluoresbrite Carboxylate YG Microspheres, Polysciences, Inc, PA). Cells were incubated with beads at 37°C for an additional 2 h ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted embryos were transferred via a hand drawn glass capillary to a 2 uL drop of M9 buffer containing ∽100 polystyrene beads 20 um in diameter (Polysciences Inc.), sandwiched between two pieces of #1.5 coverglass ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed when the cell density reached 2.5 × 106 cells per mL by using expression plasmids and polyethylenimines (PEIs, Polysciences, USA). Approximately 1.5 mg of plasmids were premixed with 4.5 mg of PEIs in 50 mL of fresh medium for 25 minutes before application ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were mounted with Aqua Poly/Mount (Polysciences) and imaged using a Zeiss LSM510 META or LSM880 laser confocal microscope under a 40x objective ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using linear polyethylenimine (Polysciences Inc.) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using polyethylenimine (PEI, Polysciences Inc.) and 150 mM NaCl using a 1:6 (w:v ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were embedded with Aqua-Poly/Mount (Polysciences). The primary and secondary antibodies used for immunostaining are listed in Appendix Table S3.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transiently transfected with polyethyleneimine (Polysciences) with different StrepII-GFP-CSPP1 constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde (PFA; Polysciences) for 10 minutes at 37°C and permeabilized in ice-cold 90% methanol (Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T17 cells were transfected using polyethylenimine (PEI, Polysciences) with helper plasmid pxx-680 (A gift from R.J ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were transfected using Polyethylenimine MAX (Polysciences Inc.) or 293fectin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The cells were fixed with 10% formaldehyde (Polysciences) for 24 hours after which the ELISA procedure mentioned above was performed using serum from each vaccinated animal.
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bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Biochemistry 2021Quote: WT HEK293T cells were transfected with polyethylenimine (Polysciences) using a DNA to polyethylenimine ratio of 1:2.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Constructs were transfected to cells using polyethylenimine (Polysciences). After 60 h of expression ...
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bioRxiv - Genomics 2020Quote: ... Cells were transfected using the PEI method (Polysciences). Media was changed 12 hours after transfection and viral-containing supernatant was collected 24 and 48 hours later ...
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bioRxiv - Immunology 2021Quote: ... The cells were transiently transfected with polyethylenimine (Polysciences) and respective vector plasmids in Opti-MEM and FreeStyle 293 medium (Gibco ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected using polyethyleneimine (PEI, Polysciences, Germany) and 150 mM NaCl using a 1:6 (w:v ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Stained cells were mounted in Aqua Polymount (Polysciences). All secondary antibodies were purchased from Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 293T cells were cotransfected using polyethyleneimine (PEI, Polysciences) with an Env-expressor plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were co-transfected using polyethylenimine (Polysciences) with the lentiviral vector ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were captured with BioMagPlus Concanavalin A (Polysciences) for 10min at room temperature and incubated with primary antibody (final concentration 1:100 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were transfected with polyethyleneimine (PEI; Polysciences). A mixture of 12 μg of the validated retroviral vectors pSIREN_EGFP-siLuc (Addgene #122287 ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected using Polyethylenimine (PEI; Polysciences), and Ishikawa and AN3CA cells were transfected using Lipofectamine2000TM (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected using polyethylenimine (PEI; Polysciences) (113 ...
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bioRxiv - Microbiology 2022Quote: ... Each sample was run twice on a BD Canto II flow-cytometer with 2 μm diameter fluorescent beads (Polysciences, Warminster, PA, USA) as a size and fluorescence standard ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Molecular Biology 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells/mL culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa PEI (Polysciences, 24765-1) per mL culture volume ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Neuroscience 2022Quote: ... mt-GCaMP6f: AAV1) and transfer plasmids were mixed and diluted in saline before mixing with saline-diluted polyethylenimine (PEI, Polysciences #23966-2) and brief vortexing ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Microbiology 2021Quote: ... 5×105 cells per well in 6-well plates were transfected with 2 μg of plasmid using 8 μg of polyethylenimine (23966, Polysciences, Inc., Warrington, PA), which was reconstituted at 1 mg/mL ...