Labshake search
Citations for Polysciences :
351 - 400 of 811 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... cells were transfected with plasmids using polyethylenimine "Max" (Polysciences, USA) with a standard method.
-
bioRxiv - Immunology 2021Quote: ... Cells were transfected using 60 μg of PEI-Max (Polysciences) with a mix of three plasmids ...
-
bioRxiv - Genetics 2019Quote: ... The surface attached cells were fixed in 4% formaldehyde (Polysciences) for 15 min ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were transfected using polyethylenimine (PEI MAX; Polysciences 24765-1) with 30μg helper plasmid pAdΔF6 ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were transiently transfected with the plasmids with polyethyleneimine (Polysciences) in Opti-MEM (Gibco ...
-
bioRxiv - Biophysics 2022Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
-
bioRxiv - Microbiology 2022Quote: ... cells were captured on magnetic ConA beads (Polysciences, PA, USA) in the presence of CaCl2 ...
-
bioRxiv - Microbiology 2022Quote: ... cells were fixed in PBS with 4% formaldehyde (Polysciences, Inc.) and 0.02% glutaraldehyde for 15 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed in PBS and mounted with Aquapolymount (Polysciences).
-
bioRxiv - Microbiology 2022Quote: HEK293T cells were transfected with plasmids using polyethylenimine (PEI, Polysciences). Media was replaced 4 h post transfection with DMEM media without antibiotics supplemented with 10 mM HEPES and virus harvested 48 h later ...
-
bioRxiv - Microbiology 2023Quote: ... cells were transfected using polyethylenimine (PEI) (#23966- 1; Polysciences, Inc.) with 5 μg of the indicated DNA constructs using a 1:5 ratio with PEI ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were transfected using linear polyethylenimine (PEI) (25k, Polysciences, Inc) at a ratio of 2:1 ...
-
bioRxiv - Cell Biology 2023Quote: HEK293T cells were transfected with plasmid DNA using polyethylenimine (Polysciences) at a final concentration of 5 μg/ml for 24 h or 48 h ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK-293T cells were transfected using homemade polyethylenimine (Polysciences, Inc.). 36h after transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were coupled to ConA-coated magnetic beads (Polysciences Europe), permeabilized and subsequently incubated with the primary antibody (EXOSC5 ...
-
bioRxiv - Bioengineering 2023Quote: ... HEK293T cells were triple transfected with PEI-MAX (Polysciences, #24765) to deliver the rep-cap or iCAP ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transfected at a cell density of 2.5×106 cells/mL by co-transfecting plasmids (1.1 mg total DNA/L cells) using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells).
-
bioRxiv - Biochemistry 2020Quote: ... VPS15 and BECN1 constructs were transfected to cells using polyethylenimine (Polysciences). After 60 h expression ...
-
bioRxiv - Cell Biology 2019Quote: ... and TOM-1 cells with “Max” polyethylenimine (Polysciences, Warrington, PA, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2021Quote: ... Both plasmids were co-transfected to Expi293 cells using PEI (Polysciences) when the cells reached a density of 2.5 × 106/ml ...
-
bioRxiv - Developmental Biology 2021Quote: ... Plasmids were transfected into HEK293F cells using PEI MAX (24765; Polysciences) and conditioned medium was collected 48-72 h later ...
-
bioRxiv - Evolutionary Biology 2020Quote: Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). We used a second-generation packaging system to generate the lentiviruses used in this study (gift from Connie Cepko Harvard Medical School) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). Following concentration ...
-
bioRxiv - Microbiology 2020Quote: ... to 0.8×10e6 cells/ml were transfected with linear PEI (Polysciences) with 397ug DNA at a 1:3 DNA:PEI ratio ...
-
bioRxiv - Genomics 2020Quote: ... into HEK293T cells using a 1 mg/ml PEI solution (Polysciences). Lentiviral supernatants were collected at 48 hours and 72 hours after transfection ...
-
bioRxiv - Cell Biology 2019Quote: ... and Cos-1 cells with “Max” polyethylenimine (Polysciences, Warrington, PA, USA) according to the manufacturer’s recommendations.
-
bioRxiv - Cell Biology 2021Quote: ... and HEK-293T cells were transfected using polyethylenimine (#23966-1, Polysciences), while the RPE-1 cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were bound to Concanavalin A beads (86057-3, Polysciences Inc), followed by permeabilization using a digitonin containing buffer ...
-
bioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). To block unspecific binding sites the fixed cells were incubated with blocking buffer containing 10% normal goat or donkey serum ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were transiently transfected with plasmid DNA using polyethylenimine (PEI, Polysciences), with a 1:3 DNA:PEI ratio ...
-
bioRxiv - Neuroscience 2022Quote: ... Mammalian cell transfections were carried out with 1 mM PEI (Polysciences) solution at 2:1 ratio μl reagent to μg plasmid DNA for 24-72H depending on downstream use ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were grown overnight and transfected using polyethyleneimine “Max” (Polysciences, Inc.) as previously described [20].
-
bioRxiv - Cell Biology 2022Quote: Plasmid transfection into HEK293T cells was performed using PEI Max (Polysciences) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were transfected using 1 mg/ml PEI MAX® (Polysciences). Media was replaced 16 h post-transfection and viral supernatants were harvested through a 0.45µm filter 48 h post transfection ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at 40-50% confluence using PEI (Polysciences Inc.) [47] and used 24 hours after transfection.
-
bioRxiv - Cell Biology 2022Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were transfected by using polyethyleneimine (PEI) transfection reagent (Polysciences). Transfected cells were further incubated at constant shaking speed for 4 days before harvesting the culture supernatant ...
-
bioRxiv - Cancer Biology 2019Quote: ... Chromatin from HT-144 cells was fixed with 1% formaldehyde (Polysciences) and prepared with Magna ChIP™ HiSens chromatin immunoprecipitation kit (EMD Millipore) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Constructs were transfected together into HEK293 cells using polyethylenimine (PEI, Polysciences) with a 24-h incubation time for protein expression ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293F cells were transfected using PEI 25K linear transfection reagent (Polysciences). Expression was performed at 37°C for 7 hours followed by a second expression phase at 30°C for 72 hours.
-
bioRxiv - Biophysics 2021Quote: ... The plasmid constructs were transfected into cells using polyethyleneimine (Polysciences Inc.) (Baldi et al. ...
-
bioRxiv - Neuroscience 2021Quote: ... Cultured cell lines were transfected with plasmids using PEI MAX (Polysciences) complexes with a DNA to PEI MAX ratio of 1:3 w/w as previously described49 ...
-
bioRxiv - Cancer Biology 2022Quote: HEK293T cells were transfected with plasmids using polyethylenimine (PEI) (Polysciences, #24765). 4.0μg total plasmid DNA was transfected for 48 h.
-
bioRxiv - Biochemistry 2022Quote: ... cells were reverse transfected using linear polyethyleneimine (25 kDa, Polysciences 23966) with LuTHy constructs and cells were subsequently incubated for 48 h ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were transiently transfected with the constructed plasmid using polyethyleneimine (Polysciences). Transfected cells were imaged using a IX83 wide-field fluorescence microscopy (Olympus ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). We used a second-generation packaging system to generate the lentiviruses used in this study (gift from Connie Cepko Harvard Medical School) ...
-
bioRxiv - Molecular Biology 2023Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...
-
bioRxiv - Biophysics 2022Quote: ... Fixed cells were then mounted with Mowiol 4-88 (Polysciences, Inc.) onto glass slides and kept at 4 °C until imaging.
-
bioRxiv - Immunology 2022Quote: ... These plasmids were transiently transfected into HEK293F cells using polyethylenimine (Polysciences) to express the corresponding proteins ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cell transfection was performed using polyethylenimine (PEI) (23866-1, Polysciences, Inc) according to manufacturer instructions.