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Citations for Polysciences :
51 - 100 of 811 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Cancer Biology 2019Quote: ... and either 2-µm YG microspheres (Polysciences; 17155-2) or 15-µm scarlet microspheres (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Calculations of relative fluorescence per cell were done by normalizing red chlorophyll fluorescence per cell to 2 μm reference fluorescent beads (catalog no. 18604; Polysciences, Warrington, PA, USA) as previously described [72] ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 2 ml 2% PFA (Polysciences, Inc., 18814). After 30 min incubation at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with 1 μg/ml DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences). Conditioned medium containing the secreted recombinant protein was collected 4 days post transfection and filtered ...
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bioRxiv - Cell Biology 2022Quote: ... were transiently co-transfected with 4xPylT/PylRS(Y306A, Y384F) and 4xPylT/EMC3(Amber[TAG])-GFP plasmids at a ratio of 4:1 using PEI “MAX” (Polysciences, USA). The cells were grown in the presence of 0.5 mM AbK (Iris Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 2 µg total DNA and 7.35 µl polyethylenimine HCl MAX transfection reagent (Polysciences, Inc). Wells co-transfected with mClover3-TDPΔNLS and HSP104 variants received 1 µg of each plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg/mL of each expression vector using 2 μg/mL Polyethyleneimine “MAX” (PolySciences) (Hirayama et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... and triple transfected into 10 15-cm plates of HEK293T cells using PEI transfection reagent (Polysciences, 23966- 2). 60 h after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... 293T media was changed and the cells were transfected using 1mg/ml of polyethylenimine (PEI) (Polysciences, 23966-2).
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...
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bioRxiv - Cancer Biology 2019Quote: ... in FACS buffer containing either 2-µm YG microspheres (Polysciences; 17155-2) or a known concentration 15-µm scarlet microspheres ...
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bioRxiv - Cancer Biology 2021Quote: ... at a ratio of 2:2:1 using polyethylenimine (Polysciences, Warrington, PA). After 18 hours of transduction ...
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bioRxiv - Cell Biology 2022Quote: ... Polyethylenimine (Polysciences, 23966-2), Opti-MEM™ (GIBCO ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were transfected with 1 μg/ml of purified plasmid DNA and 2 μg/ml of polyethylenimine (Polysciences Inc.). Cells were harvested 72 hours post transfection and frozen at −80°C until used ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with FACS buffer and fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Warrington, PA) diluted in FACS buffer (fixation buffer) ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Microbiology 2019Quote: ... 3×105 cells/well (12-well plate) or 2×106 (10-cm dish) and transfected on the following day using polyethylenimine (PolySciences).
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bioRxiv - Molecular Biology 2019Quote: ... or PEI (POLYSCIENCES 23966-2) into HEK293T cells grown on a 10cm dish (Moffat et al. ...
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bioRxiv - Synthetic Biology 2023Quote: Polyethylenimine (PEI) (Polysciences 23966-2) was used to transfect HEK293FT cells ...
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bioRxiv - Immunology 2023Quote: ... 3 μm (#19118-2; Polysciences) or 6 μm (#17145-4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent 2 μm beads (Polysciences Fluoresbrite® YG Microspheres 2.00μm 18338-5 ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Neuroscience 2022Quote: ... The seeded cells were then transfected with a total 2 μg of DNA using polyethylenimine 25 kDa (PEI, Polysciences Inc., Germany) at a 6:1 ratio of PEI to DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then grown to optical density of 1.2×106 cells/mL at 37 °C and transfected with Flag-Tagged plasmids using PEI (Polysciences, 23966-2). After 48 h of transfection ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Molecular Biology 2019Quote: ... linear mw 25,000 (Polysciences 23966-2). Cells were washed with DMEM or DMEM-F12 2-4 hours after transfection reagent was added (Longo et al. ...
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bioRxiv - Cell Biology 2021Quote: ... After fixation with 2% glutaraledhyde (Polysciences) in PBS (pH7.4) ...
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bioRxiv - Bioengineering 2021Quote: ... polystyrene latex microspheres (Polysciences, 18142-2) with our HELP gels ...
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bioRxiv - Bioengineering 2023Quote: ... 2-sulfoethyl methacrylate (PolySciences, Warminster, PA) was added to a final concentration of 5 mM ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 mg of PEI “MAX” (Polysciences) (40 kDa ...
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bioRxiv - Genetics 2020Quote: ... and PEI Max (Polysciences, Inc. 24765-2). At 72 hr posttransfection ...
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bioRxiv - Bioengineering 2021Quote: ... 2-aminoethyl methacrylate (AEMA, 3.04 g, Polysciences) (molar ratio of NHS:EDC:AEMA = 1:2:1 ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 2% paraformaldehyde solution (Polysciences, #18814-20 ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5×106 HEK293T cells were seeded into 10 cm dishes and transfected with the vector of interest using PEI (Polysciences 23966-2, Warrington, PA) along with pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
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bioRxiv - Bioengineering 2022Quote: ... A concentration of 2 μg mL-1 plasmid DNA and 2 μg mL-1 plasmid DNA linear 25kDa polyethyleneimine (Polysciences) dissolved in OptiPRO serum free medium (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: All larvae were fixed in 2 mL round bottom tubes with 2 mL of fixation solution [1.5% Formaldehyde (Polysciences, Inc.), 0.1 M PIPES (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... A 2 mm region just rostral of the hippocampus was drop-fixed in 2% PFA/2.5% glutaraldehyde (01909-100, Polysciences, Inc.) at 4°C overnight ...