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Citations for Polysciences :
301 - 350 of 811 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected using PEI-max (#24765-1, Polysciences) with 100 ng of pSNL-N2-C or pSNL-N2-Muc7 and 100 ng of pSNL-N1-”C interactors” ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected using either PEI Max (Polysciences) or the calcium phosphate method ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Immunology 2022Quote: ... were transfected into the HEK293F cells using polyethylenimine (Polysciences). The conditioned media were harvested and concentrated using a Hydrosart ultrafilter (Sartorius) ...
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bioRxiv - Immunology 2022Quote: ... were transfected into the HEK293F cells using polyethylenimine (Polysciences). The conditioned media were harvested and concentrated using a Hydrosart ultrafilter (Sartorius) ...
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bioRxiv - Molecular Biology 2019Quote: ... AD293 producer cells were transfected using PEI (Polysciences Inc.) with 10 μg of pLKO.1-puro-Scramble control (SHC002 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transfected using linear PEI ∼25,000 Da (Polysciences) as described previously (18,29).
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed with 4% EM grade Formaldehyde (Polysciences) for 5 minutes at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then mounted with Aqua poly mount (Polysciences) and analyzed on a Leica TCS-SP2 AOBS confocal microscope (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cell transfections were done using MaxPEI 25K (Polysciences) in a 3:1 MaxPEI:DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Immunostained cells were mounted in Aqua-Poly/Mount (Polysciences) and analyzed at epi-fluorescent or confocal microscopy ...
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bioRxiv - Microbiology 2021Quote: ... into Vero cell using PEI Max (MW 40,000) (Polysciences) and selected with 2.5 µg/ml Puromycin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... and HeLa cells by Polyethylenimine ‘Max’ (Polysciences, Inc., Germany). Poly (I:C ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with polyethylenimine MW 25,000 (PEI, Polysciences) at a ratio of 5 μg PEI to 1 μg DNA ...
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bioRxiv - Biochemistry 2022Quote: Adherent cells were transfected with linear polyethylenimine (PEI; Polysciences) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then transfected with polyethylineamine (PEI, 40,000MW, Polysciences) using 24μg of PEI and 8μg total plasmid per condition ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HEK293A cells were transfected transiently using polyethylenimine (PEI, Polysciences) and 10 ng per well of each of pcDNA3.1-hM4 mAChR (WT or mutant) ...
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bioRxiv - Immunology 2023Quote: ... at 5 ×106 cells/mL using PEI MAX (Polysciences) for DNA delivery ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with polyethyleneimine (PEI) (Polysciences; #23966-1) with pCMV-dR8_91 and pVSV-G packaging plasmids ...
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bioRxiv - Genomics 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery96 ...
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bioRxiv - Microbiology 2023Quote: ... 293T cells were transfected by Polyethylenimine “Max” (PEI) (Polysciences) transfection with the respective expression plasmids ...
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bioRxiv - Immunology 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were either transfected using PEI Max 40K (Polysciences) at a DNA:PEI ratio of 1:3 (w/w ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were mounted with Aqua-Poly/Mount (Polysciences, Inc.) and imaged directly in the cell imaging plates ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transiently co-transfected using PEI MAX (Polysciences) with the 5C12 hIgA2/hIgK/hIgJ plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected using linear 40 KDa polyethyleneimine (Polysciences) at a mass ratio of 1:3 (DNA:PEI) ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293T cells were transfected using Polyethylenimine “Max” (PEI) (Polysciences) in OptiMEM medium (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... lentiviruses were produced in HEK293T cells by polyethyleneimine (Polysciences)-mediated transfection using an pLV-EF1a-IRES-Puro (Addgene Plasmid #85132)-based vector expressing human ACE2 ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were then washed for 10 minutes in PBS (3 times) and stained with a 1:1 mixture of 2% aqueous osmium tetroxide (cat# 23310-10, Polysciences, Inc., Warrington, PA, USA) and 5% potassium dichromate for 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed using 3% formaldehyde and fixed cells were placed on poly-L-lysine coated multi-well slides (Polysciences Inc., Warrington, PA, USA). Cells were then blocked for one hour using phosphate-buffed saline (PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was introduced into the cells using PEI (polyethylenimine, Polysciences) at a 3:1 PEI:DNA ratio ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfections of HEK293T cells were performed with polyethylenimine (Polysciences).
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bioRxiv - Systems Biology 2021Quote: ... Cells were transfected with 25 kDa linear PEI (Polysciences Inc.) at a DNA:PEI ratio of 1:4 (Freestyle™ CHO ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transiently transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed in PBS and mounted with Aquapolymount (Polysciences).
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bioRxiv - Neuroscience 2019Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested five days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using transfection grade Polyethylenimine (Polysciences, Cat # 23966) or lipofectamine 3000 (Thermo ...
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bioRxiv - Cancer Biology 2022Quote: ... and LS174T cells done with PEI MAX (Polysciences, 24765-1). DLD-1 cells were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviruses were produced in HEK293T cells using polyethylenimine (PEI) (Polysciences)97 transfection including the packing plasmids PMDL ...
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bioRxiv - Biophysics 2022Quote: ... Cell transfection was done with PEI reagent (24765-1, Polysciences) according to instructions provided by the manufacturers ...
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bioRxiv - Cell Biology 2021Quote: ... 60% confluent HEK293T cells were transfected using PEI (Polysciences, 23966) in OptiMEM (Thermo ...