Labshake search
Citations for VWR :
251 - 300 of 315 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414). Technical replicates of 20 ng (based on RNA concentration ...
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bioRxiv - Developmental Biology 2022Quote: ... 5ml of curing agent and 45ml of Monomer (both Sylgard® 184 Elastomer Kit, VWR) were mixed intensively ...
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bioRxiv - Neuroscience 2022Quote: Frozen corpus callosa were pulverised using the Precellys Bioruptor and bead-based lysis kit (VWR). Tissue was transferred to 1.5ml Precellys beaded tubes with 300 μl of lysis buffer (5% SDS ...
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bioRxiv - Physiology 2022Quote: ... the isolation was performed according to the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA) and verified through sequencing.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... by grinding the larvae in the C1 buffer of this kit with a Precellys (VWR).
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bioRxiv - Cell Biology 2022Quote: Total RNA was prepared using the peqGOLD total RNA isolation kit (VWR, Radnor, PA, USA) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and IF1Over tachyzoites using the Zymo Quick-RNA MiniPrep kit (VWR, cat. no. 76020-636). The integrity of the extracted RNA was confirmed via an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... A temperature-controlled oil bath (Instatherm® Economy Bath/Controller Kit, Ace Glass, Inc., VWR, USA) equipped with a type J thermocouple temperature sensor was employed to control the heat treatment temperature ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracted using a PeqGold DNA Blood and Tissue Extraction kit following the manufacturer’s instructions (VWR), and PCR performed targeting both Ca ...
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bioRxiv - Physiology 2022Quote: ... Total RNA (1 μg) was reverse transcribed using qScript cDNA Synthesis Kit (VWR, Houston, TX, USA). qRT-PCR analysis was performed using SYBR Green qPCR Master Mix (VWR) ...
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... The 3’ end enriched libraries were constructed using KAPA HyperPlus Library Preparation Kit (VWR International, KK8513) with 6 cycles of PCR ...
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bioRxiv - Cancer Biology 2024Quote: Silicone elastomer (PDMS)) was mixed with a curing agent (Avantor VWR; Sylgard 184 Elastomer Kit 634165S) at a 10:1 ratio and centrifuged at 300×g for 10 min to remove bubbles ...
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bioRxiv - Cell Biology 2024Quote: ... Cellular RNA was then reverse transcribed to cDNA using Qscript cDNA Synthesis Kit (VWR, 101414-098). Two step qPCR was then ran using iQ SYBR Green supermix (BioRad ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from ∼50 mg samples with an E.Z.N.A® Plant DNA Kit (VWR, Lutterworth, UK). DNA fragment size was assessed on a 1% agarose gel in Tris/Borate/EDTA to confirm that all samples had the majority of DNA fragments >10 kilobases.
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from tissue or cell culture using the EZNA RNA isolation Kit (VWR, Spånga, Sweden) or using TRIzol reagent and RNeasy Universal Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Trichrome staining was performed on 5 μm tissue sections using Gomori’s Trichrome Stain Kit (VWR, 84000-308) according to the manufacturer’s protocol ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Total RNA from cells was isolated using the peqGOLD total RNA isolation kit (VWR, Radnor, PA, USA) according to the manufacturer’s protocol and RNA concentrations of the samples were determined with a NanoDrop 1,000 spectrometer (VWR ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted using the peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) for lines transformed with pOsCPR5.1-B construct while extraction was done according to (Edwards et al. ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination.
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bioRxiv - Molecular Biology 2024Quote: ... PeqGOLD Plasmid Miniprep Kit I (C-Line), and chemical solvents (methanol, ethylacetate, acetonitrile) from VWR (Darmstadt, Germany); deoxynucleotides (dNTPs) ...
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bioRxiv - Plant Biology 2021Quote: Leaf genomic DNA was isolated using a peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplification conditions were adjusted using foliar DNA isolated with the Plant DNA mini kit (VWR, Darmstadt, Germany). Thus ...
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bioRxiv - Genetics 2024Quote: ... The DNA was isolated from frozen ear muscle biopsies using the peqGOLD blood & tissue DNA mini kit (VWR) and following the protocol provided by the manufacturer.
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bioRxiv - Microbiology 2024Quote: Shotgun sequencing libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (VWR International, Radnor, USA), and sequenced with PE150 on an Illumina NovaSeq6000.
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bioRxiv - Microbiology 2024Quote: Total DNA was extracted from feces or biofilms using the EZNA isolation kit stool DNA (VWR for biofilms) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... We extracted DNA from stable cultures of resident communities using peqGOLD Bacterial DNA Kit (VWR #13-3450-02), then sent the obtained DNA to Eurofins for library preparation and sequencing of 16S rDNA V3-V4 region ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using the Qiagen QuantiTect Reverse Transcription Kit and analyzed using the PerfeCTa SYBR green FastMix (VWR) and a Rotor-Gene 6000 (Qiagen) ...
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bioRxiv - Genomics 2020Quote: Library preparation for each CUT&RUN antibody condition was done with a KAPA Hyper Prep Kit (VWR, 89125-040) with the PCR amplification settings adjusted to have simultaneous annealing and extension steps at 60 °C for 10 seconds ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from lysed tachyzoites using the Zymo Quick-RNA MiniPrep kit (VWR, cat. no. 76020-636). RT-qPCR was conducted using primers P20 and P21 for TgIF1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription of RNA into cDNA was performed via the manufacturer’s protocol using qScript cDNA synthesis kit (VWR, 95048100). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from the enrichment beads using IBI scientific TRI-Isolate RNA Pure Kit (VWR cat# 76006-526) and eluted into 50 μL nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and cDNA was synthesized with the peqGOLD MicroSpin Total RNA Kit (Peqlab/VWR Life Science, 12-6831) and the qScript™ Flex cDNA Kit (Quantabio ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
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bioRxiv - Molecular Biology 2021Quote: ... 1ug of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 1µg of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Tissues were homogenized with a Minilys homogenizer (Bertin Technologies, Rockville, MD) using a Precellys Lysing Kit (VWR Scientific, Radnor, PA). Total RNA was extracted from thoroughly homogenized tissues via miRNeasy Mini Kit (#217004 ...
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bioRxiv - Immunology 2020Quote: ... Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069 ...
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bioRxiv - Developmental Biology 2022Quote: RNA from embryonic kidneys was extracted by the use of the peqGold Total RNA-Kit (VWR International GmbH, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... pEhActEx was digested with AflII to remove RLUC and the CD46 and CD55 PCR products were each cloned into the backbone using a Gibson Assembly Ultra kit (VWR). The vector control plasmid was created by self-ligating pEhActEx following removal of RLUC by AflII digestion ...
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bioRxiv - Microbiology 2024Quote: ... RNA used for gene expression analysis was purified using Zymo Research Direct-zol RNA Miniprep Plus kit (VWR 76211-340) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted and purified using RNA cleanup columns (IBI scientific TRI-Isolate RNA Pure Kit, VWR cat# 76006-526), eluted into 25 μL of water and immediately visualized by ge (see “Gel-based visualization of RNA”)
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bioRxiv - Synthetic Biology 2024Quote: ... The Oxford Nanopore Rapid Barcoding Kit and R10.4 flow cell were purchased from VWR (USA, #SQK-RBK114.96 and #FLO-MIN114). For the short-reads ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... a part of the organ tissue was weight and homogenised following the protocol of the Precellys soft tissue lysing kit (CK14, Bertin Instruments, VWR, UK). The fluorescence of Cy7-psomes was measured in using a multimode microplate fluorometer (Spark ...
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bioRxiv - Genetics 2020Quote: ... centrifuged and the aqueous phase was applied to a PerfectBind RNA Colum of the Total RNA Kit (peqGOLD, VWR, Radnor, USA). Further extraction of total RNA was performed according to the kits protocol ...