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Citations for VWR :
151 - 200 of 315 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The assay plates were then sealed using a sterile polyester film (VWR, cat # 89134-432) before placing the lid to prevent the panel from dehydrating during incubation ...
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bioRxiv - Bioengineering 2021Quote: ... CQ loaded nanocarriers were purified from free CQ by 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution.
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bioRxiv - Microbiology 2021Quote: ... 10 μl of the cell lysates were resolved by standard 10% SDS-PAGE gel and electroblotted onto 0.2 mm nitrocellulose membrane (Biotrace, VWR) using a semi-dry transfer unit (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... followed by filtering the supernatant through a 0.45 μm pore membrane (sterile polyether sulfone filter, VWR North America, USA). Concentrated MS2 stocks were stored at 4°C for short durations (<2 days ...
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bioRxiv - Molecular Biology 2020Quote: ... Following removal of the glutathione beads by centrifuging in a centrifugal spin column (VWR, modified Nylon membrane, 0.45 µm) at 1.5 rcf for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... and MD40HG cultures in 0.2% FLA-YM (as above) and a no cell negative control were filtered through 0.2 μm cellulose acetate membrane syringe filter (VWR). The filtrates were flash frozen and stored at −80°C until analysis ...
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bioRxiv - Genetics 2022Quote: ... the membranes were thoroughly washed where after bands were visualized by chemiluminescence (ECL prime, Amersham, VWR, Radnor, Pennsylvania, USA) in combination with ChemiDoc Imaging Systems (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were run on 7.5-12% polyacrylamide gels for ∼1 hour at 150V and transferred to PVDF membranes (VWR) for 1 hour at 330 mA on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR, 0777), and incubated overnight with the following primary antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... The phosphoproteome samples were resolubilized in 50 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was also transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Plant Biology 2022Quote: ... The reference proteome sample was resolubilized in 30 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The library was cultured in a 96-well deep well plate sealed with a gas permeable membrane (VWR, USA). The plate was shaken (700 rpm ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Tryptic peptides were resolubilized in 30 μl of 0.05% TFA and 2% ACN in H2O filtered through 10 kDa MWCO PES membrane spin filters (VWR) at 14,000×g for 15 min and transferred to HPLC vials.
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bioRxiv - Bioengineering 2023Quote: The cell culture plate inserts (transwells) for 24 wells (PET membrane, 3.0 µm pore size) were purchased from VWR International.
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bioRxiv - Neuroscience 2024Quote: ... a suspension of MRMT-1 cells (1 × 106 cells) was combined with standard Matrigel basement membrane matrix (VWR, # CACB354234) at a 1:1 ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest of the gel containing the parasite proteins was transferred to a PVDF membrane (VWR, cat. no. PI88518). Membranes were probed with rabbit anti-F1β (Agrisera ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysis from organoids and co-culture assays was performed with peqGOLD TriFast (VWR Life Science) as recommended by the manufactures followed by RNA isolation with the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.4 µm TC Plate Insert with a PC Membrane 6 well plate (thinserts) was purchased from VWR (Radnor Pennsylvania, USA). PD-L1 ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The membranes were washed 3x in PBST at RT and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (VWR, Amersham ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Microbiology 2021Quote: ... was added to each well of a 96-well AcroPrep filter plate with 0·2 μM wwPTFE membrane (514-1096, VWR). A100 μL of plasma sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic, WG1402BOX) and then transferred to 0.45μM nitrocellulose membranes (VWR, 10120-006). Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR ...
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bioRxiv - Molecular Biology 2024Quote: Boiled protein samples were analyzed by SDS PAGE on 6% acrylamide stacking gel and 8% acrylamide resolving gel and transferred to 0.45μm nitrocellulose blotting membranes (VWR CA10061-120). Membranes were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×104 cells in 100μL media were plated in the top of the Corning filter membrane insert (VWR, Radnor, PA) sitting inside of a 24 well tissue culture plate ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Cell Biology 2020Quote: Protein extracts from total cells and eluates from immuno-precipitations were separated by standard SDS-PAGE and transferred to PVDF membranes (#10600023, VWR, USA) for the detection of Mup1-GFP ...
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bioRxiv - Bioengineering 2021Quote: ... Dye-loaded nanocarriers were purified from free dye by dialysis or 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 200 uL of the grown media was spinned down at 3000 rpm for 15 minutes in an Eppendorf 5810 tabletop centrifuge and its supernatant was filter-sterilized using a 0.2 uM membrane 96-well filter (VWR 97052-096). The rest of the unfiltered grown media was kept at 4 °C for up to 30 hours as the inoculum for the next generation ...
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bioRxiv - Cell Biology 2021Quote: ... 40% glycerol and 412mM β-mercaptoethanol) before being resolved by 8% SDS-PAGE and transferred to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Genetics 2020Quote: Quadriceps from P10 pups were dissected and total proteins extracted using radioimmunoprecipitation assay (RIPA) buffer (VWR, Radnor, PA) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2022Quote: RNAscope assay was performed on lung 5 μm FFPE sections on Superfrost Plus microscope slides (#631-9483, VWR) cut from depths consecutive to the sections mounted on Visium slides ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Cells and tissues were lysed by radioimmunoprecipitation assay (RIPA) buffer (0.2% IGEPAL CA-620, 0.02% SDS with VWR Life Science Protease Inhibitor Cocktail) ...
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bioRxiv - Systems Biology 2020Quote: ... cells were removed from their liquid media by flowing the liquid cultures that contained cells through a 0.45−μm pore filter (VWR, cellulose-acetate membrane). To ensure and verify that there were no cells remaining in the filtered media ...
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bioRxiv - Cell Biology 2022Quote: ... Samples containing 20-50 µg protein were separated by SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose or polyvinylidene fluoride membrane (10600023, VWR, Radnor, PA). After blocking the membrane for 1h in 3% BSA in Tris- buffered saline with Tween 20 (TBS-T) ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Biochemistry 2023Quote: ... 80 μg of total proteins were denatured in a buffer containing with 8 M urea and 50 mM NH4HCO3 in a 30 kDa molecular weight cut-off polyethersulfone membrane centrifugal filter unit (VWR 82031-354). Cysteines in the denatured proteins were reduced and alkylated by incubating with 20 mM DTT at 37°C for 1 hour and 55 mM iodoacetamide in the dark at room temperature for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... SigmaAldrich), and riboflavin (R4500, SigmaAldrich) were first sterile filtered (0.2 um, Media bottle filtration unit with PES membrane, VWR No. 514-0297) and added to the media prior to each experiment and the pH was adjusted to 6.7 with NaOH immediately before the start of the experiment.
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bioRxiv - Immunology 2020Quote: These assays were done using methods similar to those described (52) and Immulon 4 microtiter plates (VWR, Radnor, PA) coated with 100ng per well of S1 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Beads were then split into tubes and MMP assay +/− activated MMP (final concentration 0.73 ug/mL unless noted otherwise) and +/− Batimastat (final concentration 40 uM) (BB94, VWR) as needed for that condition ...
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bioRxiv - Biochemistry 2024Quote: ... Time curve assays were performed aerobically in 50-100 μL volumes in 1.5 mL crimp neck amber vials (VWR) at 25°C and 300 rpm using a thermomixer pro (Cellmedia ...
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bioRxiv - Microbiology 2021Quote: ... Membranes that were electrospun from 15 wt% of PVDF without dyes onto a layer of PP fabrics (VWR® Basic Protection Face Mask) were also fabricated for comparison (i.e. ...
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bioRxiv - Bioengineering 2021Quote: ... Microtiter plates were filled with 160 μl of bloodmeal per well and sealed with a membrane (Parafilm M, PM999, VWR, Amsterdam, the Netherlands) that was stretched to about 250% its original dimensions in both directions using a custom build device (Figure S1A ...
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bioRxiv - Neuroscience 2021Quote: ... Plaque assays were developed 4dpi by removal of overlay media and staining/fixation using 10% neutral buffered formalin (VWR #89370) and 0.25% crystal violet (VWR #0528).
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s recommendations and was added as a template into a one-step multiplex qRT-PCR assay using Quanta qScript XLT ToughMix with ROX dye (VWR). For that ...
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Microbiology 2024Quote: Cells (hMVEC-d or hTERT) were seeded in the upper chamber of the Transwells (10769-240, VWR insert, 24 wells, PET membrane, 3.0 µm) at a density of 3 × 105 in 100 µl of media ...