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Citations for VWR :
101 - 150 of 315 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins separated on SDS-PAGE gels were transferred to nitrocellulose membranes (VWR, #27376-991) and then probed with appropriate antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (VWR Life Science) supplemented with phosphatase inhibitor cocktail 2 (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... and total DNA content measured using the fluorometric Quantifluor dsDNA Assay (VWR) and converted to cell number for normalization.
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Cell Biology 2022Quote: ... cAMP was measured using Cayman ELISA cAMP assay (VWR, Cat #75817-364) following manufacturer recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Soft agar used in spotting assays contained 0.5% (w/v) agar (VWR). Each tile from the tile repository (FIG 1 ...
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bioRxiv - Cell Biology 2021Quote: ... a 24-well insert with an 8-μm-pore membrane Trans-wells (VWR International Ltd) was coated with 50 μl of CellMatrix/PureCol collagen mixture (1:1 ...
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bioRxiv - Cell Biology 2021Quote: ... sections were transferred to 0.4 μm polycarbonate membranes cell culture inserts (VWR, Radnor, PA, USA) and incubated in Claycomb medium supplemented with 10 % foetal calf serum ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were blocked for 30 minutes with either 5% milk or BSA (VWR, 97061-416) diluted in 1 X TBST [100 mL of 10 × 1L Stock (90 g Tris Base (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatant was passed through a syringe filter (0.2 μm cellulose acetate membrane, VWR). The filtrate was kept frozen for 48 hrs at −20°C and then thawed and centrifuged (3000 x g ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with 5 % milk powder (MP, Skim milk powder, (VWR, Radnor, PA, US)) in 1x TBST buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then imaged on a C-DiGit Chemiluminescent Western Blot Scanner (103375-240, VWR) using SuperSignal West Pico PLUS Chemiluminescent Substrate (PI34577 ...
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bioRxiv - Cancer Biology 2023Quote: ... or 6.5 mm cell culture inserts (8.0 µm pore and polycarbonate membrane, VWR #734-2744). 2 hours prior to the assay ...
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bioRxiv - Physiology 2024Quote: ... sterilized using a vacuum filtration system with polyethersulfone (PES) membrane (2 μm pore size; VWR), and stored at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... the collected supernatant was passed through triple layered nylon membranes (150 and 50 μm, VWR) and then through an 8 μm pore size filter (Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... plants were harvested and meristems were stained with 5 μM SynaptoRed membrane dye (VWR 80510-682) in DMSO ...
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bioRxiv - Bioengineering 2021Quote: ... The solution was then transferred into 12-14 kDa MWCO dialysis membranes (VWR, Chicago, IL, USA) and dialyzed against DI water for one week ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA was UV-crosslinked to the membrane by using VWR UV crosslinker (VWR, #89131-484) at 120,000 microjoules per cm2 and 100 ng/mL of the probe diluted in DIG Easy Hyb Granules Working Buffer was hybridized overnight at 42°C with gentle agitation (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... A transwell plate insert with a 0.4µm pore size bottom PET membrane (VWR, Cat# 76313-906) was placed in each well and the inoculum (MOI=5 ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting blend was then filtered through a cellulose acetate membrane (0.45 µm, VWR, #28145-481). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... An equal volume (2.5 µl) of purified RNA was dotted on Amersham Hybond N+ membrane (VWR) and crosslinked using an UV Stratalinker 2400 (Autocrosslink mode) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resolved by 8% SDS-PAGE and transferred to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25mM Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 5% non-fat milk or 5% bovine serum albumin (BSA; VWR #97062-904) in TBS before probing overnight at 4°C with primary antibodies diluted in TBS containing 0.2% Tween-20 (TBS-Tw) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with goat anti-rabbit IgG conjugated to HRP (VWR, cat. no. 102645-182) secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... Membrane blocking was performed for 1 h at room temperature in 5% bovine serum albumin (BSA; VWR) in Tris-buffered saline containing Tween-20 (TBST) ...
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 15,000 rpm for 2 min and filtered through a 0.2 μm cellulose acetate membrane (VWR). Then ...
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bioRxiv - Microbiology 2024Quote: ... The RNA was transferred using a semi-dry system to a Nytran SuPerCharge nylon blotting membrane (VWR). After transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with goat anti-mouse IgG conjugated to HRP (VWR, cat. no. 102646-160) and goat anti-rabbit IgG conjugated to HRP (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with goat anti-mouse IgG conjugated to HRP (VWR, cat. no. 102646-160) or goat anti-rabbit IgG conjugated to HRP (VWR ...
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bioRxiv - Cancer Biology 2022Quote: ... Lipid uptake was measured using the QBT Fatty Acid Uptake Assay (VWR, 10048-826) using the BioTek Synergy plate reader ...
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bioRxiv - Microbiology 2022Quote: Biofilm formation assays were performed in 96 well flat-bottomed plates (VWR 10861-562) coated with 100uL of BSA or Fg (150 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration throughout the study was determined via Bradford assay (catalogue number-VWR M172).
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bioRxiv - Biochemistry 2024Quote: Meanwhile the assay plate was centrifuged (MEGA STAR 4.0R, VWR International BVBA, Leuven, Belgium) at 500 g ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked by 5% Powdered milk (Roth, T145.3) in PBS-0.2% Tween20 (VWR Life Science, M147-1L), detected with the indicated antibodies and analyzed by quantitative near-infrared scanning (Odyssey ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a 500uL volume of each culture was filtered-sterilized (0.2 um centrifugal filter; nylon membrane; VWR 82031-358) and assayed in technical triplicate for enzymatic starch hydrolysis (see measuring amylolytic function below) ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Zoology 2021Quote: ... the samples were passed through a 5–15 μm pore size paper membrane (VWR, Cat No. 516-0813) by gravity filtration to avoid damaging the cells– M ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was cleared by centrifugation at 20,000g for 30 min and filtered through a 0.45 µm cellulose acetate membrane (VWR). In order to purify MIF the lysate was first passed over a 5-mL CM-sepharose column (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... the collected culture medium was filtered with a 0.45 μm polyethersulfone (PES) membrane (VWR International, Radnor, PA, USA) to avoid contamination by the cellular proteins ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were separated from the culture liquid by centrifugation and filtration through 0.22 µm membranes (VWR, USA). Next ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated in blocking solution [5% milk in PBST (PBS (pH 7.4) with 0.1% Tween-20 (VWR))] for 2 h and probed overnight with the following primary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was pooled together with the sucrose fraction and filtered through a 0.22 μm PES membrane (VWR) and used immediately or stored at 4°C for further purification steps.
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bioRxiv - Biochemistry 2024Quote: ... the sample was applied onto polyethersulfone membrane centrifugal filters with 3 kDa cutoff (VWR International GmbH, Darmstadt, Germany) to concentrate this fraction ...
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bioRxiv - Cell Biology 2024Quote: 25 µg of proteins were separated in SDS-PAGE gels before being transferred to nitrocellulose membranes (VWR 10600002). After ponceau staining ...
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bioRxiv - Plant Biology 2020Quote: All germination assays were performed in sterile 24-well culture plates (VWR International; 734-2779), using 500 μL of the specified solution and dry seeds (typically 20 ...
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bioRxiv - Cell Biology 2021Quote: ... a competitive ELISA cAMP assay was used (Enzo Cat #581001 or VWR Cat #75817-364). Cells expressing bPAC constructs were plated on 6-well plates ...
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bioRxiv - Biochemistry 2024Quote: In vitro assays were performed using NUNC 96-well plates (VWR International GMBH, Darmstadt, Germany) and a CLARIOstar plate reader (BMG Labtech ...