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Citations for VWR :
51 - 100 of 315 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and filtration on a 0.2 µm polyethersulfone membrane (VWR, #514-0340). The solution was then concentrated by ultrafiltration ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with 1% BSA in PBS (VWR, 76286-966) in both apical and basal chambers for 1 hour at RT with gentle shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were sterilized by filtration (0.45 μm PES membrane filters, VWR).
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bioRxiv - Molecular Biology 2024Quote: ... membranes were stripped according to manufacturer directions (VWR, cat. no. PI21059) and re-probed with mouse anti-tubulin (Developmental Studies Hybridoma Bank at the University of Iowa ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were stripped according to manufacturer directions (VWR, cat. no. PI21059) and re-probed with rabbit anti-F1β (Agrisera ...
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bioRxiv - Cell Biology 2024Quote: ... Protein bands were transferred to nitrocellulose membranes (VWR, cat# 28298-020) which were blocked with 5% non-fat milk in Tris-Buffered Saline with Tween 20 (TBST) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Physiology 2023Quote: ... fish were acclimatized for 4–5 days prior to the assay in a cylindrical assay chamber (325 mL glass dish, 10 cm × 5 cm, VWR, Radnor, PA, USA) filled with conditioned water (pH 6.8–7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was blocked with 5% bovine serum albumin (VWR, Batavia, IL) in PBS containing 0.1% Tween-20 for one hour at room temperature and then incubated in primary antibodies overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... and filtering with 0.2 μm syringe filters (cellulose acetate membrane, VWR International). All proteins were purified with desalting columns (Zeba Spin Desalting Columns ...
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bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
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bioRxiv - Cancer Biology 2020Quote: ... tissue culture plate inserts with 8.0 μm pore size polycarbonate membranes (VWR) were placed in 24-well tissue culture plates (VWR) ...
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bioRxiv - Microbiology 2021Quote: ... spheroids were grown in Corning® Matrigel® Basement Membrane Matrix (VWR). Spheroids were dissociated with Trypsin-EDTA (1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... and then transferred onto a Biotrace NT nitrocellulose membrane (27376-991, VWR). Total proteins were stained with Ponceau S solution (P7170 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... to the membrane and exposing it to autoradiography film (VWR 490001-930).
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 1X PBS (prepared from 10X concentrate; VWR International) containing 0.05% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked in 1X PBS (prepared from 10X concentrate; VWR International) containing 0.05% (v/v ...
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bioRxiv - Developmental Biology 2022Quote: ... Resulting solution was filtered through a centrifuge filter (30K PES membrane, VWR) for 7 min at 12,000 x g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was crosslinked to membranes at 2400J/m2 using UV Crosslinker (VWR) and tRNA were detected using digoxigenin-labeled (DIG ...
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bioRxiv - Physiology 2023Quote: ... membranes were cut to remove excess and Ponceau S stained (VWR K793) to confirm complete transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Immunology 2020Quote: ... Plaque assays were fixed using formaldehyde (VWR, Leighton Buzzard, UK) and stained using 0.1% Toluidine Blue (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: The assays were performed on LB medium (Lennox) plates (VWR). The E ...
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bioRxiv - Developmental Biology 2021Quote: ... and sealed around the membrane borders with oil 10 S Voltalef (VWR Chemicals). Images were acquired with 60x oil objective (NA 1.4 ...
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bioRxiv - Microbiology 2021Quote: ... and transferred to membrane (Nytran SuPerCharge) using the TurboBlotter system (VWR 89026-838). A DNA probe consisting of the 5’ 750 bp of the PPS gene was produced by PCR (primers 16/17) ...
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bioRxiv - Cell Biology 2020Quote: ... and sealed around the membrane borders with oil 10 S Voltalef (VWR Chemicals). Images were acquired with 60x oil objective (NA 1.4 ...
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bioRxiv - Systems Biology 2022Quote: ... and flowed them through a 0.2 µm pore filter (VWR, cellulose-acetate membrane). We then directly proceeded to measure the ROS production rate in the supernatant as described in the paragraph “measuring extracellular ROS production rate”.
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bioRxiv - Pathology 2021Quote: ... then the supernatants were filtered through a 0.2 μm cellulose acetate membrane (VWR) into sterile 1.5 mL Eppendorf tubes ...
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bioRxiv - Molecular Biology 2022Quote: ... or 10% SDS-PAGE and wet transferred to Nitrocellulose membranes (VWR, CA10061-084) at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Molecular Biology 2024Quote: Membranes were blocked with the LI-COR Biosciences Intercept Blocking buffer (VWR International) for one hour at room temperature and incubated with primary antibodies overnight at 4 °C on a rocker ...
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bioRxiv - Molecular Biology 2024Quote: ... Nitrocellulose membrane was then incubated with goat Anti-mouse (102971-154, VWR International) and goat Anti-rabbit (103011-498 ...
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bioRxiv - Bioengineering 2024Quote: ... filtration through a polyethersulfone (PES) membrane filter with 0.45 µm pore size (VWR), Ultracentrifugation at 4°C and 120,000 xg for 2 hours ...
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bioRxiv - Pathology 2024Quote: ... Gradient gels were then transferred to 0.45-μm nitrocellulose membranes (VWR, #27376-991) for 1 hr at 75 V in ice-cold 20% methanol in 1X Tris-glycine buffer (Bio-Rad ...
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bioRxiv - Pathology 2024Quote: ... The gradient gels were transferred onto 0.45-μm nitrocellulose membranes (VWR, #27376-991) for 1 hour at 75 V using ice-cold 20% methanol in 1X Tris-glycine buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... the C18 membrane was activated using 20 μL LC-MS grade MeOH (VWR), cleaned by 20 μL 80% (v/v ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: Toxicity-neutralisation assays were performed on LB medium (Lennox) plates (VWR). E ...
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bioRxiv - Pathology 2021Quote: ... the assay buffer was 1% bovine serum albumin (VWR Chemicals, UK) in Tris Buffered Saline (TBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assay was conducted on a black walled 96-well petriplate (VWR) according to manufacturer’s instructions and fluorescence was measured on an Agilent BioTek Microplate Reader (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and filtered through a 0.22 µM polyethersulfone (PES) membrane filter (VWR) and stored in aliquots at −80 ...
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bioRxiv - Cell Biology 2020Quote: ... The cytosol fraction in the supernatant was concentrated using 10K Polyethersulfone (PES) membranes (VWR) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Membrane was washed in TBST and blocked in TBST + 5% milk (VWR # 10128-600) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Collected supernatants were then passed through a 5.0µm acrodisc versapor membrane syringe filter (VWR). The filtrate was then pelleted at 16,000xg for 2min ...
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bioRxiv - Microbiology 2021Quote: ... cultures were separated by membranes of different pore sizes in 6-well plates (VWR). Inserts with membrane pore size of 0.4 µm (VWR 10769-192 ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Physiology 2024Quote: Twenty-four-well plates and 8-μm pore-size polyvinylpyrrolidone free polycarbonate membranes (VWR) were used ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated in blocking buffer consisting of 5% bovine serum albumin (BSA; VWR) dissolved in 1X Tris-buffered saline (TBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then centrifuged and the supernatant filtered through a 0.22 μm PES membrane (Avantor, VWR). For each clone a maximum growth rate was estimated from 5 measurements of optical density (OD ...
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bioRxiv - Cell Biology 2024Quote: ... the lentivirus was filtered through a cellulose acetate membrane (0.45 µm, #28145-481, VWR), and 250 µl of media containing the lentivirus were added to a 60mm dish of the indicated cells along with polybrene (8 µg/ml ...