Labshake search
Citations for Zymo Research :
301 - 318 of 318 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... and prey (pGADT7) constructs were co-transformed into the yeast strain AH109 using Frozen-EZ Yeast Transformation II Kit (Zymo Research, T2001). The resulting transformants were spotted on SD (−Leu ...
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bioRxiv - Cell Biology 2020Quote: ... mixed 1:1 with 100% ethanol and the mix was transferred to a column of the Direct-zol RNA MiniPrep Plus Kit (Zymo Research, #R2070). The following purification steps were done according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Bioengineering 2022Quote: Yeast competent cells were transformed with polycistronic constructs containing fluorescent proteins using the Frozen-EZ yeast transformation II kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast cells in mid-log phase were co-transformed with pDEST32/pDEST22::AtNHR2B or pDEST32::HopG1/ pDEST22::AtNHR2B using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). Transformed yeast cells were plated on Double Drop Out (DDO ...
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bioRxiv - Biochemistry 2021Quote: ... which were then grown in liquid culture prior to purification of the plasmid library using a ZymoPure II Endotoxin-Free Midiprep kit (Zymo Research, Irvine, CA). An analysis of our final DMS data suggest this library contains at least 7,614 of 7,686 possible variants ...
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bioRxiv - Cell Biology 2022Quote: ... followed by (ii) RNA purification and on-column-digestion of genomic DNA using the Direct-zol RNA Miniprep kit (Zymo Research, Freiburg, Germany), and (iii ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid and fosmid DNA was purified with either the Zyppy Plasmid Miniprep kit or ZymoPURE II Plasmid Midiprep kit (Zymo Research, Irvine, CA). Following restriction digests or PCR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... High yield pure plasmid DNA preparations were subsequently obtained from 100 mL overnight LB broth cultures using the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA).
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was isolated via the Direct-zol RNA MiniPrep kit including Zymo-Spin II and DNAse restriction step (R2050, Zymo research, Irvine, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL, Zymo-IIICG = 60μL, all from Zymo Research), and then allowed to incubate on the column at 37°C for 45 min ...
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bioRxiv - Zoology 2024Quote: ... These amplified products were then purified as above and restriction enzyme digested CCHa1R and CCHa2R sequences were ligated into pcDNA3.1+ and pBudCE4.1 mammalian expression vectors followed by bacterial transformation and plasmid DNA purification to obtain a large quantity of receptor constructs using ZymoPURE II Plasmid Midiprep Kit (Zymo Research, Tustin, CA, USA) following the manufacturer recommendation ...
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bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...