Labshake search
Citations for Zymo Research :
201 - 250 of 318 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Competent LLE cells were then created using the Zymo Research Frozen-EZ Yeast Transformation II Kit (Zymo#T2001). When the component cells containing either Integrated TEVsubstrate or TEVp were complete ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid assays were performed as described in the Frozen-EZ Yeast Transformation II™ (Zymo Research). For the interaction of TaNF-YA3-D ...
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bioRxiv - Genetics 2024Quote: Yeast two-hybrid assays were performed as described in the Frozen-EZ Yeast Transformation II™ (Zymo Research). The coding sequences of TabHLH27-A1 were cloned into the prey vector (pGADT7) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were suspended in 6 ml of 1:1 mix of 1 M sorbitol: YPDplus medium (Zymo Research) and incubated at 30°C for 1 h ...
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bioRxiv - Systems Biology 2024Quote: ... These pools were each transformed into competent 10beta cells prepared with a Mix and Go kit (Zymo Research), selected with LB+Carb media ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pipetted the mix into a column provided in the Direct-zol RNA Miniprep Plus kit (Zymo Research, #R2072) and followed manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The resulting plasmid was extracted from yeast using the Zymoprep yeast miniprep kit version II (Zymo Research, California, USA), amplified in E ...
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bioRxiv - Microbiology 2022Quote: ... (Hayashi et al., 2009): DL70 cells were made chemically competent (Frozen-EZ Yeast Transformation II Kit, Zymo Research, #T2001) and were transformed with a linear DNA fragment consisting of the mScarlet-I sequence and a hygromycin resistance cassette flanked by 500-600 bp of homology arms on both sides for homologous recombination into the genome downstream of the native sad1 gene ...
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bioRxiv - Genomics 2019Quote: ... we estimated the complexity as >500K and extracted the plasmid using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research).
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bioRxiv - Genomics 2021Quote: ... and plasmids from both before and after selection were isolated using the Yeast Plasmid Miniprep II Kit (Zymo Research).
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bioRxiv - Genomics 2021Quote: Competent cells for each yeast strain were prepared using the Frozen-EZ Yeast Transformation II Kit™ (Zymo Research) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
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bioRxiv - Biochemistry 2022Quote: ... into the Saccharomyces cerevisiae wild-type strain CEN.PK2-1D using the Frozen-EZ yeast transformation II kit (Zymo Research) according to according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and two community controls (ZymoBIOMICS Microbial Community DNA Standard and ZymoBIOMICS Microbial Community DNA Standard II (Log Distribution) (Zymo Research ...
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bioRxiv - Bioengineering 2022Quote: DNA of the active population was extracted from yeast cells using Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) and amplified in two steps ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified for transfection into mammalian cell lines using a ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Biochemistry 2023Quote: ... These cells were cultured in LB media and plasmids were purified using ZymoPURE II Plasmid Midiprep kit (Zymo Research). Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... and sequence confirmed (Sequetech) and then maxi-prepped per the manufacturer’s protocols (ZymoPure II Plasmid Maxiprep Kit, Zymo Research). Plasmids were sterile filtered using a 0.22-μm syringe filter and stored at 4 °C.
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bioRxiv - Genetics 2023Quote: ... C shaker for 12h-16h prior to harvesting for plasmid purification using ZymoPURE II Plasmid Midiprep kit (Zymo D4200). Based on the colony forming units from the small volumes in the bacterial plates ...
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bioRxiv - Microbiology 2024Quote: ... For GFP2 and GFP4 vectors successfully cloned enhancer constructs were further isolated with ZymoPure II Maxiprep kit (Zymo research).
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bioRxiv - Synthetic Biology 2024Quote: ... Colonies were scraped with 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203); the extracted plasmid DNA was then precipitated with isopropanol ...
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bioRxiv - Biochemistry 2024Quote: ... DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004). VH sequences were amplified by PCR and submitted to Genewiz for Amplicon NGS sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Colonies were collected with 1.5mL sterile DDW and extracted using Zymoprep Yeast Plasmid Miniprep II (Zymo Research, CAT #D2004).
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bioRxiv - Synthetic Biology 2021Quote: ... Complementary plasmids were co-transformed into competent MC1061ΔpspF cells carrying the reporter plasmid pLY54 (prepared using the Mix & Go E. coli Transformation Kit from Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... suspensions were transferred into ZR BashingbeadTM lysis tubes containing a mix of 0.1 mm and 0.5 mm zirconium beads (Zymo Research) and 750 μL lysis buffer ...
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bioRxiv - Developmental Biology 2020Quote: Combinations of the AD or BD vectors were co-transformed into yeast using the Frozen-EZ II kit from Zymo Research and using empty vectors as negative controls ...
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bioRxiv - Microbiology 2019Quote: ... Transformation of yeast cells was performed according to manufacturer's guidelines using the Frozen‑EZ Yeast Transformation II Kit (Zymo Research) and cells were selected on complete supplement mixture (CSM ...
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bioRxiv - Biophysics 2019Quote: ... Plasmid were isolated using an E.Z.N.A Plasmid MiniPrep Kit (Omega Bio-teck; Norcross, GA) or a ZymoPure II Plasmid Midiprep Kit (Zymo Research; Irvine, CA) per manufactures recommendations ...
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bioRxiv - Synthetic Biology 2019Quote: ... Then 1 mL of the culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit ...
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bioRxiv - Biochemistry 2021Quote: ... Quick-RNA miniprep Kit (Cat. No. R1054) and ZymoPURE II Plasmid Maxiprep Kit (Cat. No. D4203) were obtained from Zymo Research ...
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bioRxiv - Pathology 2021Quote: ... cerevisiae strains PLY240 (MATa his3Δ200 leu2-3,112 trp1Δ901 ura3-52 suc2Δ9 trk1Δ51 trk2Δ50::lox-kanMX-lox) [55] was transformed with the constructed pYES2sh plasmids using Frozen-EZ Yeast Transformation II kit (Zymo Research Europe GmbH ...
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bioRxiv - Microbiology 2021Quote: ... used in this study has a log abundance distribution of different bacterial species and fungi ranging from 108 to 102 cells (ZymoBIOMICS Microbial Community Standard II, ZYMO RESEARCH EUROPE GMBH ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection-grade plasmid DNA was isolated from each flask using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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bioRxiv - Bioengineering 2020Quote: ... Around 50 ml of yeast culture was harvested and DNA was extracted using Zymoprep Yeast Plasmid Miniprep II (Zymo Research). Two rounds of PCR were performed to amplify the coding sequence of the selected proteins and attach standard Illumina sequencing adaptors ...
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bioRxiv - Microbiology 2021Quote: ... plasmid DNAs were isolated using the Zyppy plasmid mini prep kit or ZymoPURE II plasmid maxiprep kit (Zymo Research, USA); recombinant plasmids were verified by restriction enzyme digestion and Sanger DNA sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... eluted RNA is spun out into fresh tubes and a second elution with water is performed (Zymo-I = 30μL, Zymo-II = 50μL, Zymo-IIICG = 60μL). To the eluate ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids expressing eCFP-SGD and eGFP-MDR were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Resultant yeast strains were cultivated at 30 □ ...
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bioRxiv - Immunology 2023Quote: ScFv encoding plasmids were recovered from yeast cultures by miniprep with the Zymoprep yeast plasmid miniprep II kit (Zymo Research) as previously described(42) ...
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bioRxiv - Genetics 2023Quote: ... coli-derived Ydj1-CXXX plasmid library was transformed into yWS304 (ydj1Δ) via Frozen-EZ Yeast transformation II Kit by Zymo Research (Irvine ...
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bioRxiv - Synthetic Biology 2023Quote: ... A maximum of 96 PCR reactions were pooled at a time and cleaned with Zymo-Spin II columns (Zymo Research). Pooled samples were sequenced with the NGSelect Amplicon sequencing service of Eurofins Genomics.
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bioRxiv - Cell Biology 2023Quote: MAGIC YKO was constructed with a two-step transformation using the Frozen-EZ Yeast Transformation II Kit (T2001, Zymo Research) following the microscale protocol in 96-well format ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed and corrected recombinant plasmids were digested with Not I for linearization and then transformed into Po1fΔKu70 using the Frozen-EZ yeast transformation II Kit (Zymo Research). Transformants were screened on YNB plates supplemented with auxotrophic supplements containing appropriate amino acids and placed in an incubator at 30 °C for 2-3 day ...
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bioRxiv - Synthetic Biology 2024Quote: DNA templates for expression in PUREfrex were prepared either in plasmid form using ZymoPURE II Plasmid Midiprep Kit (Zymo Research) or as linear expression templates (LETs) ...
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bioRxiv - Genetics 2024Quote: ... The library was transformed into yWS2542 (ram11 ydj11) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA) per the manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pQE60::trpCp variants were transformed into the ΔtrpD JW1255-1 strain (BW25113) from the KEIO collection 47 using the Mix & Go! protocol from Zymo Research (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... The reaction is incubated in the thermocycler at 50 degrees for 30 minutes and 2μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Bioengineering 2021Quote: ... 2μL of assembled reactions were transformed into 20 μL of competent Stbl3 generated by Mix and Go! competency kit (Zymo) and plated on agar plates supplemented with appropriate antibiotics ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were prepared and transformed with plasmids using the Mix and Go™ kit (Zymo Research, Irvin, CA, USA) according to the manufacturer’s indications ...