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Citations for Zymo Research :
251 - 300 of 318 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Bioengineering 2022Quote: ... cerevisiae strain EBY100 (ATCC MYA-4941) (Boder & Wittrup, 1997) using frozen-EZ yeast transformation II kit (Zymo Research, Cat. No. T2001) and grown on SD-CAA agar plates for 3 to 4 days ...
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bioRxiv - Molecular Biology 2021Quote: ... one each with the insert in genomic and in antigenomic orientation for plasmid stock preparation using ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... The Variovorax CL14 genes contained in Vectors 1 and 2 were inferred by isolating plasmid from these clones using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and Sanger sequencing the insert ends using primers JMC247 and JMC270 (Supplementary Table 19) ...
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bioRxiv - Synthetic Biology 2020Quote: ... yeast strain was used to make competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research).
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bioRxiv - Bioengineering 2019Quote: ... 1.5-mL aliquots of cell culture were used as input in minipreps with the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Sequence verified and purified plasmids were transformed into yeast strain W303α using the Frozen-EZ Yeast Transformation II Kit (Zymo research) according to the manufacturer’s instructions and plated on YNB-URA dropout plates ...
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bioRxiv - Microbiology 2021Quote: ... were prepared and transformed with the Y2H plasmid constructs by using the Frozen-EZ Yeast Transformation II kit (Zymo Research, USA) and following the manufacturer-supplied protocol and recommendations ...
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bioRxiv - Genomics 2021Quote: ... Clones were verified by Sanger sequencing (Quintara Biosciences) prior to retransformation and maxiprep using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Cell Biology 2022Quote: Testing and control combinations of plasmids were co-transformed into yeast AH109 strain using the Frozen-EZ Yeast Transformation II Kit (ZYMO, T2001) and cultured on a SD/-Leu-Trp selective plate at 30 °C for 2–4 days ...
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bioRxiv - Immunology 2022Quote: DNA was obtained from at least 107 yeast per round of selection using a Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Primers were designed to amplify the Aga2 leader peptide through to the beginning of the β2M sequence ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Plant Biology 2022Quote: ... YES3-Gate-OPT3 construct or YES3-Gate lacking the cDNA insert were transformed into appropriate yeast line using the Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformants were selected for uracil prototrophy on YNB medium containing 6.7% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from yeast libraries using Zymoprep-96 Yeast Plasmid Miniprep kits or Zymoprep Yeast Plasmid Miniprep II kits (Zymo Research) according to standard manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from the yeast library using Zymoprep-96 Yeast Plasmid Miniprep kits or Zymoprep Yeast Plasmid Miniprep II kits (Zymo Research) according to standard manufacturer protocols ...
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bioRxiv - Bioengineering 2019Quote: DNA associated with the mutants recovered from the fourth round of screening was isolated using a Zymoprep yeast plasmid miniprep II kit (Zymo Research). For both the TOM22 and c-Kit screens ...
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bioRxiv - Immunology 2021Quote: scFv encoding plasmids were recovered from yeast cultures by yeast miniprep with the Zymoprep yeast plasmid miniprep II kit (Zymo Research). Isolated DNA was transformed into NEB5α strain of E ...
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bioRxiv - Immunology 2021Quote: ... and enriched yeast plasmid libraries were extracted using Zymoprep Yeast Plasmid Miniprep II kit according to the manufacturer’s instructions (Zymo Research D2004).
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bioRxiv - Biochemistry 2022Quote: ... and transformed into the EBY100 yeast surface display strain [14] that had been made chemically competent using the Frozen EZ-Yeast Transformation II Kit (Zymo Research). For each single site-directed mutant library transformed yeast were plated onto three each Synthetic Dropout minus Tryptophan (SD -Trp ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... yeast (PUP1/pup1Δ::kanMX) with a Magic marker selection were made using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For maximum representation of mutant human gene clones transformed in the strain ...
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bioRxiv - Plant Biology 2022Quote: Bait and prey constructs were co-transformed into the yeast strain AH109 using the yeast transformation kit (Frozen-EZ Yeast Transformation II Kit, Zymo Research). The resulting transformants with appropriate positive and negative controls were spotted on SD (−Leu ...
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bioRxiv - Plant Biology 2022Quote: ... Competent cells of these strains were prepared using the Frozen EZ Yeast Transformation II Kit™ (Zymo Research, Irvine, CA, USA) and transformed with a multigene plasmid carrying DlCYP87A4 and ATR2 or human P450scc ...
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bioRxiv - Bioengineering 2023Quote: ... or a modified pNTA vector with V5 tag into EBY-100 yeast using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Transformed yeast were passaged once in minimal glucose medium (SDCAA ...
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bioRxiv - Molecular Biology 2023Quote: Total genomic DNA was extracted from ∼0.2 mg (i) spinach and (ii) mixed-species Teklad samples using a Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Following extractions ...
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bioRxiv - Genomics 2023Quote: ... All colonies from each reaction were scraped from the plate and the CROP-seq-opti-Puro-T2A-GFP-sgRNA plasmid library was extracted using ZymoPURE II Plasmid Midiprep Kit (Zymo, D4200). The lentiviral library was generated as stated above with extended virus production time ...
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Genomics 2024Quote: ... The transformed library was grown overnight in 50 mL of liquid Luria Broth (LB) + 50 μg/mL Ampicillin and purified using the ZymoPURE II Plasmid Midiprep kit (Zymo D4200) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in 50-100 mL of LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE II Plasmid Midiprep Kit (Zymo #D4201) by following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Synthetic Biology 2024Quote: ... colonies were scraped with 50 mL 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203). These plasmid libraries constitute the “post-selection libraries.”
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bioRxiv - Synthetic Biology 2023Quote: ... were transformed into Saccharomyces cerevisiae VL6-48(Yamanaka et al., 2014) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). The correct promoter insertion was screened by PCR using primers upstream and downstream of the target site (Table S4) ...
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bioRxiv - Biochemistry 2024Quote: ... cerevisiae strain BY4742 with endogenous Ycf1 knockout (Horizon Discovery) were transformed following the Frozen-EZ Yeast Transformation II protocol (Zymo Research). Transformed yeast strains were grown for 48 hours on YNB-His agar plates at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL of ligated plasmid was then transformed into competent BW23473 cells (made using the Mix & Go E. coli Transformation Kit & Buffer Set, Zymo Research) and plated on LB + amp plates and grown overnight ...
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bioRxiv - Bioengineering 2021Quote: ... coli were made chemically competent following either the Inoue method84 or using the Zymo Research Mix & Go! Protocol (Zymo Research T3002). E ...
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bioRxiv - Immunology 2021Quote: ... 5µl of each reaction including a negative control without scFv was subsequently transformed into 100 µl Fast Transformation of Mix & Go Competent Cells (Zymo T3001) each and plated on LB plates containing Ampicillin ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL each reaction was transformed into 100 μL DH10B and transformed according to the Mix and Go Transformation kit (Zymo Research).
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bioRxiv - Synthetic Biology 2023Quote: ... Constructs were circularized with vector backbone by Gibson assembly and introduced to XL-1 Blue Mix and Go chemical competent cells (Zymo Research), miniprepped (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... pEXG2 suicide vector containing 700-bp sequences of the flanking regions of the selected gene was directly inserted into competent SM10λpir (Mix&Go competent cells, Zymo Research Corporation) and subsequently selected on LB-Agar supplemented with 50 μg/mL kanamycin /15 μg/mL gentamicin ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast expression vector constructs or empty vector were transformed into yeast Saccharomyces cerevisiae WAT11 (Pompon et al., 1996; Truan et al., 1993) by using Yeast Transformation II Kit (ZYMO, CA, USA) and screened on synthetic SD-dropout medium lacking uracil (SD-Ura ...
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bioRxiv - Plant Biology 2019Quote: Bait and prey constructs were co-transformed into the yeast strain AH109 using the yeast transformation kit (Frozen-EZ Yeast Transformation II Kit™ - Zymo Research). Y2H assays were performed using the Matchmaker 3 system (Clontech) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted and stored at −20°C for at least six hours prior to extraction using Zymo Yeast Plasmid Miniprep II (Zymo Research # D2004), following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were verified by Sanger sequencing and purified with the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA). Two micrograms of plasmid DNAs were used to transfect small-scale HEKT293 cultures and optimize expression conditions both via mRuby2 visualization and western blot analysis ...
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bioRxiv - Bioengineering 2023Quote: Wild type 4D5 and 5A12 VH and VL scFv gene fragments were purchased from Twist Bioscience and transformed into the BamHI digested pYD1 plasmid via homologous recombination (Boder and Wittrup, 1997; Chao et al., 2006) using Frozen-EZ Yeast Transformation II (Zymo Research, T2001). 4D5 scFv was transformed in a HA-VL-218-VH-FLAG orientation and 5A12 scFv as HA-VH-218-VL-FLAG ...