Labshake search
Citations for Zymo Research :
151 - 200 of 318 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: The plasmids from sorted libraries were extracted using Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). The xylanase inserts were amplified by PCR ...
-
bioRxiv - Molecular Biology 2022Quote: Transformations of med15Δ strains were conducted using the Frozen-EZ Yeast Transformation II kit (Zymo Research) with modifications ...
-
bioRxiv - Biochemistry 2023Quote: ... which were expanded for maxiprep using the ZymoPure II maxiprep kit (Zymo Research, catalog no. D4204). Lentivirus was prepared as described for the CRISPR screen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmid libraries were isolated from cells pellets using ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203) per manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... coli JM109 cells and plasmids were extracted using ZymoPURE II midiprep kits (Zymo Research group, California).
-
bioRxiv - Microbiology 2023Quote: Plasmids were retrieved from yeast using the Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research: D2004). The protocol was modified according to Starr et al. ...
-
bioRxiv - Genomics 2023Quote: ... and plasmid DNA was extracted by using the Zymopure II plasmid midiprep kit (Zymo research #D4201).
-
bioRxiv - Genomics 2023Quote: ... and plasmid DNA was extracted by using the Zymopure II plasmid midiprep kit (Zymo research #D4201). Transfection of plasmid DNA (2.5μg vector + 1μg of transposase plasmid ...
-
bioRxiv - Plant Biology 2024Quote: ... The constructs were cotransformed into yeast strain AH109 using Frozen-EZ Yeast Transformation II kit (Zymo), in accordance with the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... Colonies were collected and the plasmids were extracted with Zymoprep Yeast Plasmid Miniprep II (Zymo Research). For the next rounds of mutagenesis ...
-
bioRxiv - Genomics 2024Quote: ... The plasmid pool was extracted and purified using ZymoPURE II Plasmid Maxiprep Kit (#D4202, Zymo Research).
-
bioRxiv - Cell Biology 2024Quote: ... and plasmid DNA was prepared using the ZymoPURE II Plasmid DNA Maxiprep kit (Zymo Research D4202). Three electroporations were mixed proportionally to their electroporation efficiency for a combined total library coverage of ∼27-fold ...
-
bioRxiv - Cell Biology 2021Quote: ... The plasmid DNAs were transformed and amplified in Mix & Go Competent Cells-Strain HB 101 (Zymo Research ...
-
bioRxiv - Synthetic Biology 2022Quote: ... All constructs were cloned into Escherichia coli TOP 10 strain using Mix&Go kit (Zymo Research) as indicated by the manufacturer ...
-
bioRxiv - Genetics 2023Quote: ... The injection mix was prepared by mixing the purified plasmid DNA (Zymo D4016 PLASMID MINIPREP-CLASSIC) with DNA lad-der (1 kb Plus DNA Ladder ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and transformed into MegaX cells made chemically competent (Mix & Go E. Coli Transformation Kit, Zymo Research). The top 8 variants of each library selection were constructed ...
-
bioRxiv - Systems Biology 2024Quote: ... and cloned these into 10beta cells made competent via the Mix-and-go kit (Zymo Research). Clones from these were arrayed in a 96-well plate and screened for chloramphenicol resistance that would indicate presence of the Gateway counterselection cassette ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Plasmid DNA was purified with the ZR Plasmid Miniprep or ZymoPure II Midiprep kit (both Zymo Research). The integrity of all plasmids was verified by Sanger Sequencing (GENEWIZ Europe).
-
bioRxiv - Microbiology 2021Quote: ... DNA products were isolated from liquid cultures using either a ZymoPURE II Plasmid Maxiprep Kit (Zymo Research) or a Macherey-Nagel Nucleobond Xtra Maxi Kit.
-
bioRxiv - Systems Biology 2022Quote: ... and plasmid DNA was extracted with multiple ZymoPURE II Plasmid Midiprep columns (Zymo Research cat. no. D4200). Library coverage was estimated by counting the number of bacterial colonies on a 1:1,000 dilution plate and a 1:10,000 dilution plate ...
-
bioRxiv - Genomics 2020Quote: ... Endo-free plasmids used for mammalian transfection were prepped using ZymoPURE II Plasmid Midiprep (Zymo Research Corporation) from 50 mL Mach1 (ThermoFisher ...
-
bioRxiv - Biochemistry 2020Quote: ... DNA was recovered from 30 individual clones using a Zymoprep yeast plasmid miniprep II kit (Zymo Research) and sequenced ...
-
bioRxiv - Bioengineering 2022Quote: ... Final plasmids were maxipreped using the ZymoPURE II Plasmid Maxiprep kit (Zymo Research, Cat No./ID: D4202) and sanger sequenced in preparation for embryonic microinjection ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and plasmid DNA was extracted using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research, Irvine, CA, #D4201).
-
bioRxiv - Immunology 2022Quote: ... Plasmids were isolated from expanded bacterial colonies using the ZymoPURE™ II Plasmid Kit (Zymo Research #D4203). Ten μg of isolated plasmid was transfected into HEK293T cells using Lipofectamine® 2000 Transfection Reagent (Invitrogen #11668-019) ...
-
bioRxiv - Genomics 2022Quote: ... plasmid DNA was harvested from the 1L culture using the ZymoPURE II Plasmid Gigaprep (Zymo Research, #D4204). Before prepping ...
-
bioRxiv - Microbiology 2022Quote: pAd/SARS-CoV-2-S1Beta was amplified and purified using ZymoPURE II plasmid maxiprep kit (Zymo Research). For Expi293 cell transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... Plasmids were prepared for injection using ZymoPURE II endotoxin-free midiprep kit (Zymo Research Corp, Irvine, CA). Cas9/sgRNA and donor plasmids were diluted to concentrations of 300ng/μl for injection ...
-
bioRxiv - Immunology 2022Quote: ... plasmid DNA from ~107 yeast were extracted using Zymoprep II Yeast Miniprep Kit (Zymo Research; Irvine, CA) from each round of selection and the unselected library ...
-
bioRxiv - Synthetic Biology 2023Quote: Plasmids were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). The resulting strains were then tested by secologanin and tryptamine feeding in appropriate media using the same protocol as in vivo assays of CrSTR and MsSTR ...
-
bioRxiv - Molecular Biology 2023Quote: Plasmids from the yeast cells were extracted using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: 96-well PCR plates with 50 µL of DH10B ‘Mix & Go Competent Cells’ (prepared using Zymo T3001) in each well were prepared beforehand so that high-throughput transfers could be done using multichannel pipettes or the Tecan Fluent ...
-
bioRxiv - Immunology 2022Quote: ... The assembled plasmid was directly transformed into competent SM10λpir using Mix&Go competent cells (Zymo Research Corporation) and plated on selective LB plates containing 50 μg/mL kanamycin and 15 μg/mL gentamicin ...
-
bioRxiv - Immunology 2019Quote: ... The assembled plasmid was directly transformed into competent SM10λpir using Mix&Go competent cells (Zymo Research Corporation) and plated on selective LB plates containing 50 µg/mL kanamycin and 15 µg/mL gentamicin ...
-
bioRxiv - Developmental Biology 2019Quote: ... The mix was then treated with a Zymo DNA Clean and Concentrator-5 Kit (Zymo research #D4014). ATAC-seq libraries were constructed in the same manner as that described above for ChIP-seq libraries.
-
bioRxiv - Synthetic Biology 2020Quote: ... Frozen aliquots of these cells were prepared with the “Mix & Go!” Transformation Kit (Zymo Research, Irvine, CA). All restriction enzymes ...
-
bioRxiv - Immunology 2021Quote: ... Amplicons were purified directly from PCR reaction mix by using DNA Clean & Concentrator Kits (Zymo Research, #D4013) or recovered from agarose gel by using Zymoclean Gel DNA Recovery Kit (Zymo Research ...
-
bioRxiv - Genomics 2023Quote: ... 2 µl adapter filling mixture containing 0.375 mM 5-Methylcytosine dNTP Mix (Zymo Research, cat. no. D1030), 0.75 µl of Thermopol Reaction buffer (New England Biolabs ...
-
bioRxiv - Plant Biology 2019Quote: All constructs were transformed into yeast using Frozen-EZ Yeast Transformation II reagents (Zymo Research Corp., Irvine, CA) to introduce pEG202 constructs into yeast strain RFY206 (mating type A) ...
-
bioRxiv - Bioengineering 2019Quote: ... Sequences were cloned into pCS4306 by gap repair using the Frozen-EZ Yeast Transformation II Kit (Zymo Research) according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2020Quote: ... ACE2 mutant library yeast display plasmids were rescued using the ZymoPrep Yeast Plasmid Miniprep II kit (Zymo Research). Rescued plasmids were amplified via electroporation into 10G Supreme E ...
-
bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA was transformed into chemically competent EBY100 using the Frozen-EZ yeast transformation Kit II (Zymo Research). Trp-deficient SDCAA and SGCAA medium was used for culturing cells and for inducing cell surface protein expression for cells containing the pCTCON vector and Leu-deficient SDSCAA1 (-Leu ...
-
bioRxiv - Immunology 2021Quote: ... Plasmids containing TCR libraries were purified using a ZymoPURE II Plasmid Maxiprep Kit (D4202, Zymo Research, Irvine, CA). The TRBC-p2A-TRAL insert was amplified using a KAPA HiFi HotStart PCR Kit (7958897001 ...
-
bioRxiv - Bioengineering 2019Quote: ... Plasmid DNA was transformed into chemically competent EBY100 using the Frozen-EZ yeast transformation Kit II (Zymo Research).
-
bioRxiv - Immunology 2021Quote: ... plasmid DNA was isolated from the sorted yeast populations using a Zymoprep Yeast Miniprep II Kit (Zymo Research). Stellar cells (Takara ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL, Zymo-IIICG = 60μL, all from Zymo Research), and then allowed to incubate on the column at 37°C for 45 min ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Resultant plasmids were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research). Corresponding yeast strains were cultivated at 30□ ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... Transformation was carried out by electroporation for libraries48 and using the Frozen-EZ Yeast Transformation II Kit (Zymo) for individual constructs.
-
bioRxiv - Microbiology 2023Quote: ... Competent yeast cells were prepared and transformed with the Frozen-EZ Yeast Transformation II Kit (Zymo Research, USA), following the manufacturer’s instructions ...
-
bioRxiv - Zoology 2023Quote: ... high titer plasmid DNA was isolated using a ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, Irvine, USA) and used for transfection of mammalian cells utilized in the functional heterologous receptor assay.