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Citations for Zymo Research :
6151 - 6200 of 6653 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: We isolated genomic DNA from all samples using the Zymo Quick DNA mini-prep Plus Kit (Zymo Research; Cat. no. D4068). The samples were amplified and prepared for sequencing as described previously (Gilbert et al ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from 1 × 106 A549 cells treated with 0.5 % (v/v) DMSO or 5 µM FICD inhibitors (C22 or C73) for approximately 24 hours using an RNA miniprep kit (Zymo Research) and quantified using a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Genetics 2024Quote: ... recovered for 1 hour at 30°C with shaking and then grown for 12-16 hours at 30°C prior to plasmid preparation using ZymoPURE II kits (Zymo Research). To generate the final homology-directed repair template (HDRT ...
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bioRxiv - Genetics 2024Quote: ... Sequences were confirmed by whole plasmid next-generation sequencing (Plasmidsaurus) before large-scale plasmid preparations were made using ZymoPURE II Kits (Zymo Research). The plasmids were eluted in H2O and endotoxin removal (Zymo ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from 100 mg of ground tissue following the protocol of the Quick-RNA Miniprep kit (Zymo Research). The integrity of the RNA samples was assessed using the RNA Integrity Number (RIN) ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from 12-day old gametophytes using Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA). Primers were designed using SnapGene with elongation factor 1 (Ceric.09G081400 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cells were harvested via centrifugation and plasmids were purified using the ZymoPure II Plasmid Maxiprep Kit (Zymo Research cat. no. D4203) with a maximum of 75 ml culture per column and eluted in water heated to 50C prior to elution ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg frozen (maximum 1 month old) lysates were used for 2C RNA preparation in each Zymo-Spin IIICG column (Quick-RNA MiniPrep kit, Zymo #R1055). The published protocol was followed for the rest of the assay ...
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bioRxiv - Plant Biology 2020Quote: ... and then both ribosome-bound RNA and denatured total RNA samples were purified with RNA Clean & concentrator-25 kit (Zymo Research, USA). RPFs were size-fractionated on 15% urea polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: ... the 50 µL aliquot plus 5 µL of 10% (w/v) SDS was purified using the Zymo RNA clean and concentrator kit (Zymo Research; R1017). The total RNA was rRNA-depleted with the Ribo-Zero plant leaf kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed in TRIzol buffer and RNA was isolated using RNA Clean Concentrate Kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... Per sample 500 ng of genomic DNA were bisulfite converted using the EZ-96 DNA methylation Gold Kit (Zymo research, Irvine, USA) according to the kit’s manual ...
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bioRxiv - Genetics 2021Quote: DNA bisulfite conversion was performed directly starting from cell pellets (a maximum of 1×105 cells per sample) using the EZ DNA Methylation-Direct kit (Zymo Research #D5021) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We ran the libraries out on a 1.5% agarose gel and extracted 300-800 bp fragments using a Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, USA). Then ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from Buffy coat fractions and subsequently underwent bisulfite conversion using the EZ DNA Methylation kit (Zymo Research, Irvine, CA). Methylation was then assayed using the Infinium HumanMethylation450 BeadChip (Illumina Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted from each supernatant with the Zymo Quick-DNA/RNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... run on a 2% gel as before and the 150-210bp size range was excised and purified using Zymoclean Gel DNA recovery kit (Zymo Research D4007). DNA concentration was determined by qubit using the dsDNA Broad Range assay and prepared for sequencing on an Illumina HiSeq2000 machine using a single end 100bp protocol.
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from EV isolation supernatants (i.e. EV-depleted media) was extracted using Zymo ZR urine RNA isolation kit (Zymo Research, Irvine, CA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: RNA and DNA were extracted from the same frozen cell pellets using the ZR-Duet DNA/RNA MiniPrep kit (D7001, Zymo, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... those in the correct size range were extracted and purified using a Zymoclean gel DNA recovery kit (Zymo Research, Irvine, CA, USA) and Sanger sequenced (Quintara Biosciences ...
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bioRxiv - Genomics 2020Quote: ... 10 ng of the sonicated and size selected DNA was bisulfite converted using the EZ DNA Methylation-Lightning Kit (Zymo Research, D5031) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The final NET-seq library running at ∼150 bp was extracted and further purified using the ZymoClean Gel DNA recovery kit (Zymo Research D4007). Sample purity and concentration was assessed in a 2200 TapeStation and further deep sequenced in a HiSeq 2500 Illumina Platform.
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bioRxiv - Plant Biology 2020Quote: ... The yeast expression vector constructs or empty vector were transformed into yeast Saccharomyces cerevisiae WAT11 (Pompon et al., 1996; Truan et al., 1993) by using Yeast Transformation II Kit (ZYMO, CA, USA) and screened on synthetic SD-dropout medium lacking uracil (SD-Ura ...
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bioRxiv - Molecular Biology 2020Quote: ... Immediately following digestion the reaction was stopped in the binding buffer from a Zymo ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205) and purified following the protocol in the kit ...
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bioRxiv - Genomics 2021Quote: ... all the resulting H3K4me1 and H3K4me2 ChIP DNAs and 200 ng of the corresponding input DNA were subjected to bisulfite conversion according to the EZ DNA Methylation-Direct™ Kit (Zymo Research). Then ...
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bioRxiv - Genetics 2021Quote: ... All samples were randomized into 96-well plates for RNA extraction with the Direct-zol-96 RNA Kit (Zymo Research, Irvine, CA). Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen ...
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bioRxiv - Genetics 2020Quote: 200ng of liver genomic DNA per sample was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research, Irvine, CA). Standards using mouse genomic DNA of known percentage methylation (0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from the pellets and the highly infected samples used the Zymo Quick-RNA kit (Zymo Research LLC, Irvine, CA). Three samples with unfiltered hemolymph ...
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bioRxiv - Immunology 2021Quote: ... or from paraffin-embedded human IgD or IgA myeloma tissue sections (obtained from the University of Arkansas for Medical Science) using Quick-DNA™ FFPE Kit (Zymo Research). Recombined Sμ–σδ ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was purified with phenol-chloroform and subjected to bisulfite conversion according to the EZ DNA Methylation-Direct™ Kit (Zymo Research). Then ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Some DNA extracts that showed high levels of degradation were size-selected using Select-a-Size column or bead kits (ZYMO, Irvine, CA). High molecular weight ...
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bioRxiv - Synthetic Biology 2021Quote: Plasmids were isolated from 5-10 mL yeast cultures either by alkaline lysis (39) or using Zymo Yeast Miniprep Kit I (Zymo Research D2001). Plasmids were then recovered into DH10B ElectroMax E ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from three biological replicates of E/I networks composed of Ngn2 #2 and Ascl1 #1 (DIV 49) with the Quick-RNA Microprep kit (Zymo Research, R1051) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and total RNA was isolated by either phase separation with chloroform and isopropanol or using the Zymo Direct-zol RNA Miniprep kit (Zymo Cat #R2050). One-step qRT-PCR was performed using the Invitrogen EXPRESS One-Step Superscript qRT-PCR kit (Thermo Fisher Cat #11781200 ...
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bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
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bioRxiv - Biochemistry 2021Quote: ... at 10 m/s for 90 s to lyse cells and then using the Zymo Research Direct-Zol RNA MiniPrep Plus kit (Zymo, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA). Small RNAs were prepared by GenXPro (GenXPro GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA).
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bioRxiv - Microbiology 2020Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Genetics 2020Quote: ... The bisulfite conversion was performed on 100 ng of sperm DNA using the EZ DNA Methylation-Gold kit (Zymo Research, Freiburg, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... A total of 500 ng of genomic DNA was bisulfite-converted (BSC) using manufacturer-recommended EZ DNAm kits (Zymo Research; Irvine, California). After conversion ...
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bioRxiv - Genetics 2020Quote: ... and genomic DNA (gDNA) of surviving cells was isolated using a genomic DNA cleanup kit according to manufacturer instructions (Zymo Research, D4065). For Illumina sequencing and screening analysis ...
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bioRxiv - Genetics 2021Quote: ... The adaptor-ligated DNA samples were subjected to bisulfite conversion by the EZ DNA Methylation-Gold kit (Zymo Research, Irvine, CA, USA) and amplified by PCR with the KAPA HiFi HotStart Uracil+ ReadyMix kit (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from PPN was extracted using the TransZol reagent (TransGen Biotech, China) in combination with the Direct-Zol RNA mini prep kit (Zymo Research, USA). cDNA was produced from 500 ng of total RNA with EasyScript Reverse Transcriptase (TransGen Biotech Cat #AE101 ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA from ∼106 parental or sorted CEM/R5 cells or CD4 lymphocytes was purified using Quick-gDNA MiniPrep Kit (Zymo Research, USA). The CXCR4 target regions were PCR-amplified using 200 ng of genomic DNA ...
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bioRxiv - Microbiology 2021Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Bacterial 16S primers amplify the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Microbiology 2021Quote: RNA from homogenized virus-inactivated lung and brain tissues of SARS-CoV-2 infected animals was extracted using the Direct-zol RNA MiniPrep Kit (Zymo Research R2051) following the manufacturer’s instructions ...