Labshake search
Citations for Zymo Research :
6351 - 6400 of 6653 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Genomic DNA (gDNA) was isolated from two G1 adults per mutant line using Zymo Quick-DNA MicroPrep kit (Zymo Research, Irvine, CA), and the yield was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: ... ∼15 ng of genomic DNA from each sample was first spiked in with in vitro methylated lambda phage genomic DNA (0.2%) as controls and was then subjected to bisulfite conversion (EZ DNA Methylation-Direct Kit, Zymo Research Cat# D5020). Half of the bisulfite converted DNA was used for low input WGBS analysis (for 5mC+5hmC profiling) ...
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bioRxiv - Genomics 2023Quote: 10 ng of gDNA isolated from GFP+/DAPI-FACS-sorted cell populations was first spiked with lambda phage controls and was bisulfite-converted with the Zymo EZ DNA Methylation Direct Kit (Zymo Research, D5020) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from GFP+/DAPI-FAC-sorted transient transfected HEK293T cells with ZR-Duet DNA/RNA MiniPrep Kit (Zymo Research, D7001). RNA-seq libraries were prepared using SMARTer Stranded total RNA-seq Kit v3 (Clontech ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The rRNA-depleted and DNAse-treated RNA was then cleaned with the Zymo RNA Clean and Concentrate-5 kit (Zymo Technologies, USA) and eluted in 8 uL nuclease-free water.
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bioRxiv - Biochemistry 2023Quote: ... DNA of 86 ovarian cancer patients was isolated from paraffin-embedded normal tissue with Quick-DNA FFPE Miniprep kit (Zymo Research, USA); other DNA samples were isolated from blood using PureLink Genomic DNA Mini Kit (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The cDNA was size selected using denatured polyacrylamide gel electrophoresis followed by dPAGE purification (small-RNA PAGE Recovery Kit, Zymo Research #R1070). The cDNA was then amplified using Q5-Ultra II DNA polymerase (New England Biolabs #M0544 ...
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bioRxiv - Paleontology 2024Quote: ... qPCR was used to calculate the absolute number of bacterial cells in each sample by targeting the 16S rRNA gene using a Femto Bacterial DNA Quantification Kit (Zymo Research International). DNA sequencing was also carried out on a negative control (no contamination was found) ...
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bioRxiv - Molecular Biology 2024Quote: The RNA was extracted and purified using the Directzol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research; Cat# R2052) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: RNA from biological duplicates for each condition (more than 30,000 cells for each sample) was extracted using the Quick-RNA Miniprep Kit (Zymo; Cat. No. R1055). Libraries were prepared from total RNA (15 ng per sample ...
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bioRxiv - Genomics 2024Quote: ... Total genomic DNA was extracted from thallus fragments using the ZR Fungal/Bacterial DNA Miniprep Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Five hundred nanograms of each DNA sample and control DNA were bisulfite-treated (BST) using the EZ DNA Methylation-Lightning Kit (Zymo Research, # D5030) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled in Tris-EDTA containing 1% SDS and 12% β-Mercaptoethanol before RNA was extracted using a Direct-zol RNA Microprep kit (Zymo Research). The extracted RNA was then prepared for RT- qPCR analysis to examine RB1 ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm successful gene insertion and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Neuroscience 2024Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Vendor-designed primers for bacterial 16S amplified the V3-V4 region ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids were sanger sequenced by GeneWiz (Azenta Life Sciences) to confirm presence of desired mutation and MaxiPrepped using the ZymoPURE II™ Plasmid Maxiprep kit (Zymo, #D4202) before transfection into Expi293™ cells.
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from the mixture of colonies from each plate using a Quick-DNA HMW MagBead Kit (Zymo Research, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Libraries were generated by the Emory Integrated Genomics Core (EIGC) as follows: RNA was isolated using the Quick-RNA MicroPrep kit (Zymo,11-328M). 2000 cell equivalent of RNA was used as input to SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... ssDNA of the bound phages was collected using a modified protocol from the Quick-DNA/RNA Miniprep Kit (Zymo Cat No. D7011). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted by phenol-chloroform for the ZNF469 profiling to keep the small DNA fragments and with the DNA Clean & Concentrator-5 kit (Zymo Research, D4004) for the H3K27ac profiling ...
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bioRxiv - Genetics 2024Quote: ... GMSM-K - 6 non-risk and 7 risk genotype for rs11119348 and 4 each genotype for rs661849) using Quick-DNA/RNA Miniprep kit (Zymo Research #D7001) followed by DNase treatment using manufacturer’s instructions (Thermo Fisher Scientific #AM1907) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids were then transformed into this yeast strain using the standard LiAc method (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and selected for on SD-trp ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The inserts were verified with PCR and Sanger sequencing and then transformed into the yeast hosts using either the standard LiAc protocol (Gietz and Schiestl 2007) or the Zymo yeast transformation kit (Zymo research T2001) and plated onto SD-leu media.
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bioRxiv - Molecular Biology 2024Quote: ... Fragments were then separated by gel electrophoresis and the band corresponding to digested vector was extracted using a Zymo kit (Zymo cat#D4002) and eluted into 20 μL nuclease-free water.
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bioRxiv - Physiology 2024Quote: ... WT and Rnlsmut NIT-1 cells were used for RNA isolation using Zymo Quick-RNA miniprep plus kit (Zymo Research cat. R1058). Novogene Corporation Inc ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted from faecal samples using the ZymoBIOMICS DNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, California, USA). DNA was then bisulfite-converted using the Zymo EZ DNA Methylation-Gold Kit to convert unmethylated cytosines to uracil and then thymine (Zymo Research ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from different tissues (gills, gonads, skin, liver, eyes, muscle) from one male individual by using the Quick-RNA MiniPrep plus Kit (Zymo Research, USA). Tissues were placed in tubes filled with 800 μl DNA/RNA shield and lysed via bead beating (speed 4 M/S ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from one of the samples stored at -80 °C using the Quick-RNA Fungal/Bacterial MicroPrep kit (Zymo Research, USA). The quality of the RNA was evaluated with a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... 75 μl of nuclease-free water was added to the mix followed by 270 μl of RNA binding buffer (RNA Clean and Concentrator-5 column kit, Zymo Research, R1015) and 400 μl of 100% ethanol ...
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bioRxiv - Microbiology 2024Quote: ... the bacterial samples were subjected to RNA extraction according to the manufacturer’s protocol (Zymo Quick-RNA Fungal/Bacterial Kits, Zymo Research, Irvine, CA). After a purity check of RNA extract quality using nanodrop and Agilent 5200 Fragment Analyzer (Agilent) ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from day 7 BORCS6 knockdown and non-targeting control i11-mNC neurons using the Direct-zol-96 MagBead RNA kit (Zymo Research, R2103). Manufacturer protocol was followed ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using flanking primers and the resulting PCR products were purified using a DNA Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA). STm 14028s carrying the pKD46 plasmid (64 ...
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bioRxiv - Genomics 2024Quote: ... 1987) and purified using a Zymo-Spin I-96 Plate and ZR-96 Clean and Concentrator Kit (Zymo Research Corporation, CA, USA) following the protocol of Rutherford et al ...
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bioRxiv - Physiology 2024Quote: Simultaneous isolation of genomic DNA and total RNA from liver tissues was performed using the ZR-Duet DNA/RNA Mini Prep kit (Zymo Research, California). RNA was treated with DNase during the isolation process ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research, #D4060). The preparation of sequencing libraries for ribosome profiling was conducted via the NEBNext Multiplex Small RNA Library Prep kit for Illumina according to the manufacturer’s protocol (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were treated with 0.5 mg/ml proteinase K at 65 ℃ for one hour and purified with Zymo ChIP DNA Clean Concentrator kit (Zymo Research D5205) as the manufacturer’s manual and quantified by QUBIT ...
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bioRxiv - Molecular Biology 2024Quote: ... Bisulfide conversion of 300 ng genomic DNA was carried out using the EZ DNA Methylation-Lightning™ Kit (Zymo Research, California, USA). The pyrosequencing assay was designed in the PyroMark Assay Design 2.0 software (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The aqueous phase was retained for RNA purification using the Zymo RNA Clean & Concentrator-5 kit following manufacturer directions for the capture of small RNA fragments with slight modification (Zymo Research, R1013). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from ten 10 μm-thick FFPE sections using the Quick-RNA Mini Prep Plus Kit (Zymo Research, cat# R1057) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The pre-adenylated linker was eluted in 6 µl H2O following the clean-up using the Oligo Clean & Concentrator Kit (Zymo Research, D4060).
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bioRxiv - Genomics 2024Quote: Viral RNA was extracted from the swab samples using the Zymo Quick-DNA/RNA Viral MagBead Kit (Zymo Research, Orange, CA, USA), and qPCR was performed using the AgPath-ID One Step RT-PCR Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: RNA was extracted according to protocol using Zymo Quick-RNA Fecal/Soil Microbe Microprep Kit (Cat# R2040, Zymo Research, Irvine, CA, USA). Samples were lysed by bead beating using Vortex Genie-2 set at max speed for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from previously collected and deidentified paraffin embedded PV patient biopsies using the Quick-RNA FFPE MiniPrep kit (ZR1008, Zymo, Irvine, CA). RNA concentration and purity were assessed using spectrophotometry ...
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bioRxiv - Bioengineering 2024Quote: ... animals were euthanized and liver tissue was collected from the median lobe from each animal for gDNA extraction (Quick-DNA/RNA Zymo Kit #R2131). ddPCR analysis performed following same methods as cell studies with efficiencies reported relative to mouse Tfrc reference copy assay (Applied Biosystems 4458366).
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bioRxiv - Evolutionary Biology 2024Quote: DNA was isolated using a modified phenol-chloroform extraction method (Davies et al., 2013) and then cleaned with DNA Clean and Concentrator kits (Zymo, Irvine, CA). Cleaned genomic DNA was sent to the University of California Davis Genome Center for library preparations using pooled Super-High-Throughput Shotgun sequencing for shallow WGS (mean 6x coverage ...
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bioRxiv - Bioengineering 2024Quote: ... merolae strains were harvested at mid-log phase by centrifugation (10 min at 2000 x g) and total genomic DNA was extracted from algal pellets using a Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, USA) and a FastPrep-24 5 g bead beating grinder and lysis system (MP Biomedicals ...
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bioRxiv - Developmental Biology 2024Quote: ... and then the DNA was purified by alkaline hydrolysis (to remove the RNA templates) followed by DNA oligo purification kits (Zymo Research, D4006). The only difference is that the forward primer in the limit-cycle PCR as well as the reverse transcription steps ...