Labshake search
Citations for Zymo Research :
6001 - 6050 of 6653 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 250 µL of the sample was transferred to a fresh tube and RNA was extracted using a Direct-zol RNA microPrep kit (Zymo Research). All RNA samples were treated to remove DNA using a DNase Treatment and Removal kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was prepared from whole embryo pellets or sorted nuclei using the Quick-DNA Miniprep Plus Kit(D4069; Zymo Research). mRNA was prepared from whole embryos using the RNeasy Mini Kit (74104 ...
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bioRxiv - Plant Biology 2022Quote: ... Competent cells of these strains were prepared using the Frozen EZ Yeast Transformation II Kit™ (Zymo Research, Irvine, CA, USA) and transformed with a multigene plasmid carrying DlCYP87A4 and ATR2 or human P450scc ...
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bioRxiv - Microbiology 2022Quote: ... In vitro transcripts were gel purified using 8 M urea 10% PAA and the ZR small-RNA PAGE Recovery Kit (Zymo Research). The in vitro transcribed RNAs carry three additional G nucleotides at their 5΄ ends originating from the T7 promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA from the input control and IP samples was isolated using the ChIP DNA Clean and Concentrator Kit (#D5205, Zymo Research) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of genomic DNA was bisulfite converted using the Zymo EZ-96 DNA Methylation kit (Zymo Research, Irine, CA, USA). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... using 1.4-mm glass beads (Omni International, Inc.) and a beadruptor (Omni International, Inc.) RNA was extracted using the DIRECT-zol Mini RNA Extraction kit (Zymo Research) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Equal volumes (at least 30 µL) of each culture were pooled and DNA was extracted using the YeaStarTM genomic DNA kit (ZYMO research).
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bioRxiv - Bioengineering 2023Quote: ... or a modified pNTA vector with V5 tag into EBY-100 yeast using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Transformed yeast were passaged once in minimal glucose medium (SDCAA ...
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bioRxiv - Immunology 2022Quote: ... FFPE scrolls were processed for DNA and RNA extraction using a Quick-DNA/RNA FFPE Miniprep Kit with on-column DNase digestion for the RNA preps (Cat. # R1009; Zymo Research). RNA was assessed for mass concentration using the Qubit RNA Broad Range Assay Kit (Cat ...
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bioRxiv - Biochemistry 2022Quote: ... The rRNA-depleted RNA samples were purified using the Zymo RNA Clean & Concentrator kit combined with DNase treatment on a solid support (Zymo Research). cDNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cell lysates using the Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, USA) according to the mnufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were then size selected by gel purifying DNA on a 10% TBE gel and cleanup was performed using Zymo DNA Cleanup kit (Zymo # D4014) and the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Total RNA was isolated from the received fungal pellet using conventional TRIzol method followed by column purification using Quick RNA Plant MiniPrep Kit (Zymo Research). The qualities and quantities of the isolated RNA were checked on NanoDrop followed by Agilent TapeStation using High Sensitivity RNA ScreenTape.
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bioRxiv - Developmental Biology 2023Quote: ... Genomic DNA extraction and sodium bisulfite conversion was performed using the EZ DNA Methylation-Direct Kit (Zymo Research Cat. No. D5020) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from frozen tissues using Direct-zol RNA Miniprep plus kit following the manufacturer’s protocol (Zymo Research - R2073). The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA ...
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bioRxiv - Molecular Biology 2023Quote: Total genomic DNA was extracted from ∼0.2 mg (i) spinach and (ii) mixed-species Teklad samples using a Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Following extractions ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated using the trizol method as described by the manufacturer’s protocol using the Directzol RNA isolation kit (Zymo Research, Germany). The RNA concentration was determined by UV measurement ...
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bioRxiv - Microbiology 2023Quote: ... DNA and RNA were coextracted from filters at Colorado State University using ZymoBIOMICS DNA/RNA Miniprep Kit (Zymo Research Cat. # R2002) coupled with RNA Clean & Concentrator-5 (Zymo Research Cat ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from homogenized colon tissue (fresh, stored in RNAlater or Trizol) or isolated crypts using the Quick-RNA Miniprep Kit (R1055, Zymo Research), or for samples in Trizol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was purified from a volume of lysate equivalent to 15 mg of tissue (525 uL) using a Quick-RNA Miniprep kit (Zymo Research). 500 ng of total RNA was used to generate libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA from each fraction was first precipitated in EtOH/C2H3NaO2 and then purified through RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Reaserach, R1016). RNA distribution profiles were analyzed by qRT-PCR.
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bioRxiv - Cancer Biology 2023Quote: ... excised the gel slices between 17 and 34 nucleotides and recovered RNA from PAGE gel by ZR small-RNA™ PAGE Recovery Kit (Zymo). Purified RNA was treated with PNK ...
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bioRxiv - Cancer Biology 2023Quote: ... Initial genomic library distributions were assessed on a 1.5% agarose gel and purified using a ZR-96 Genomic DNA Clean & Concentrator-5 Kit (Zymo Research, D4067). Library concentrations were quantified using the Quant-iT™ dsDNA Broad Range Assay Kit (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM CaCl2 or both chemicals for 16 h (see Plant materials, growth and stress assays) using the ZR Plant RNA MiniPrep Kit (Zymo Research). RNA sequencing was performed at The Mantoux Institute for Bioinformatics of the Nancy and Stephen Grand Israel National Center for Personalized Medicine ...
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bioRxiv - Plant Biology 2023Quote: ... microdissected material of around 80 gynoecia (sections of around 24 paraffin blocks with 3 or 4 embedded inflorescences) was pooled in a single tube and RNA extraction was performed with Direct-zol RNA MicroPrep kit (Zymo Research). The integrity and concentration of extracted RNA of each sample was measured using a NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DpnI-treated inverse PCR vector and PCR product inserts were column purified using a DNA Clean and Concentrate Kit (Zymo Research). Inserts were added with 2-fold molar excess to 75 ng of the vector ...
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bioRxiv - Immunology 2023Quote: ... incubated for 15 min at room temperature and either frozen at -80°C or directly followed by RNA isolation using a Direct-zol RNA microprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was prepared in a volume of 20 µL consisting in 2 µL of the template DNA and 18 µL of Femto Bacterial qPCR Premix (Femto Bacterial DNA Quantification Kit, Zymo Research). The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Metagenomic DNA and RNA were extracted starting from 350 µL of DNA/RNA shield swab sample (previously vortexed) and following a modified protocol of ZymoBIOMICS DNA/RNA Miniprep kit (Zymo Research) in which the lysis was performed only chemically using 700 µL of ZymoBIOMICS DNA/RNA lysis buffer (2 volumes of lysis buffer per 1 volume of sample) ...
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bioRxiv - Genomics 2023Quote: Two hundred and fifty nanograms of linker-ligated genomic DNA were subjected to sodium bisulfite conversion for 210 min at 64°C using the EZ DNA Methylation Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... using an in-house Tn5 transposase (IMBA-IMP-GMI Molecular Biology Services) and an EZ-96 DNA Methylation-Gold Mag Prep kit (Zymo Research) for bisulfite conversion ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... All colonies from each reaction were scraped from the plate and the CROP-seq-opti-Puro-T2A-GFP-sgRNA plasmid library was extracted using ZymoPURE II Plasmid Midiprep Kit (Zymo, D4200). The lentiviral library was generated as stated above with extended virus production time ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA template for probe synthesis was generated by PCR amplification using T7 primers and purified using a DNA clean and concentrator kit (Zymo research). Antisense RNA probes were synthesized by in vitro transcription with either DIG-11-UTP (Sigma ...
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bioRxiv - Neuroscience 2022Quote: RNAlater-preserved samples were homogenized in PBS and total RNA was extracted from samples using the Quick-RNA Miniprep Kit (Zymo Research). RNA extracts were evaluated for concentration and purity using a Nanodrop 8000 instrument (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... At the indicated time points we harvested cells and extracted RNA using the Direct-zol-96 RNA kit (Zymo #11-331H). Samples were then sent to Novogene for bulk RNA-seq.
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bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from neocortical regions of slice cultures using Trizol reagent followed by RNA Clean & Concentrator kit (Zymo research R1014). cDNA was synthesized with 0.5 μg of RNA using the cDNA Synthesis Kit (Bio-Rad 170-8891 ...
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bioRxiv - Systems Biology 2023Quote: ... to exclude small fragments (less than about 200bp) and the DNA was eluted from the gel slices using the Zymoclean Gel DNA Recovery Kit (Zymo, #D4001), followed by quantification (Qubit ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... before being spun down (10k rpm, 2 minutes) and resuspended in 80 μL of digestion buffer (included in the YeaStar RNA Kit, Zymo Research). Cells were then stored on ice until ready for RNA extraction ...
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bioRxiv - Immunology 2023Quote: ... DNA obtained from anti-OVA hits were then cut out from agarose gels and amplicons from heavy and light chains were purified separately by gel extraction (Zymoclean Gel DNA Recovery Kit, Zymo Research).
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bioRxiv - Genomics 2023Quote: ... Tissue was then homogenized with steel beads and total RNA was extracted using the Direct-zol™ RNA MicroPrep kit from Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... The genomic DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit following the protocol of the manufacturer (Zymo Research). DNA was amplified by 30 cycles of a three-step PCR (98℃ for 10 sec ...
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bioRxiv - Evolutionary Biology 2022Quote: RNA was extracted from 30 μL of each virus inoculum using a Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s protocol ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... was added to 200 μl of homogenized tissue (see DNA extraction) and processed using the Direct-zol RNA Miniprep Kit (Zymo Research) with on-column DnaseI digestion ...
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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bioRxiv - Molecular Biology 2023Quote: RNA for RNA-seq was obtained by processing cerebellar tissue or CGN cultures with Trizol reagent followed by clean up on the Zymo Direct-zol RNA miniprep kit (Zymo #R2052). RNA purity was measured to ensure samples had A260/280 and A260/230 values > 1.9 ...