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Citations for Zymo Research :
6101 - 6150 of 6653 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using the manufacturer’s instructions using a YeaStar Genomic DNA kit (Catalog D2002, Zymo Research, Irvine, California, USA). Genomic DNA was subject to a PCR to check for the insertion of the corresponding tag or the CHC KO.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells expressing the iRS3 reporter 3h post-induction using the Direct-zol™ RNA MiniPrep kit (Zymo). After extraction ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from infected cells 72 h post infection using Quick-DNA Miniprep kit (Zymo Research, catalogue no. D3024).
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bioRxiv - Genomics 2024Quote: ... An aliquot of 250 ng DNA from each sample was bisulfite converted using the EZ DNA Methylation kit (Zymo Research, #D5001) according to manufacturer’s instructions for Illumina DNA methylation arrays ...
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bioRxiv - Plant Biology 2024Quote: Preparation of RNA samples from seedlings of the indicated genotypes and growth conditions was performed using a Quick-RNA MiniPrep Kit with on-column DNase I treatment (Zymo Research). cDNA synthesis was performed with 1.5 µg of total RNA using a Superscript IV First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... contains the Arabidopsis thaliana cytochrome P450 reductase I gene, ATR1) (Urban et al., 1997) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For plant transient expression ...
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bioRxiv - Microbiology 2024Quote: A whole-stranded transcriptome RNA library was generated using the Zymo-Seq RiboFree Total RNA kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Two micrograms of DNA were subjected to sodium bisulfite conversion using an EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA). Genomic regions were subsequently amplified using EpiTaq HS (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was collected over a time course of 24 hours and isolated using a Quick-DNA/RNA™ Miniprep kit (Zymo) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µl of RNase free water was added to the mixture and transcribed RNA was eluted using the RNA Clean & Concentrator kit (Zymo Research). Samples were assessed for their length and concentration by Bioanalyzer (Agilent ...
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bioRxiv - Zoology 2024Quote: ... and post-aestivating (55 days old) female beetles were extracted using the Quick-RNA Tissue/Insect Kit (Zymo Research, Freiburg, Germany). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of ethanol were added to each of the samples before eluting the RNA using a Direct-zol RNA kit (Zymo Research) using manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Following MASC PCR validation using the MASC PCR primer set described in Supplementary Data 1 and BAC extraction using the ZR BAC DNA Miniprep Kit (Zymo Research) based on the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of every amplicon from the same RT product was pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products for both pairs of primers were pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated from freshly-grown colonies using the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using the Nextera DNA Flex Library Prep as recommended by the manufacturer (Illumina ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from 1*106 WT or sorted SLAMF6-KO NK-92 cells using an RNA Micro Kit (Zymo Research). After PolyA selection libraries were prepared and lllumina sequencing of 20-30 million paired-end reads performed ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and RNA was isolated using Direct-zol RNA miniprep Kit (Zymo research, cat. no. R2050). RNA was quantified and processed for cDNA synthesis using Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria expressing the correct plasmid were grown in bulk and high-purity plasmid was extracted using ZymoPURE Plasmid Midiprep kit (Zymo research) and verified by sequencing of the coding region of the influenza virus segment.
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bioRxiv - Physiology 2024Quote: ∼250 ng of total RNA from each sample was used as input to prepare libraries for total RNA-seq with Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The nested PCR products were then cleaned using a Zymo 96 well Clean and Concentrator Kit (Zymo Corp., Irvine, CA, USA).
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bioRxiv - Synthetic Biology 2024Quote: ... at which point cells were harvested and total DNA was extracted using a Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research). qPCR was performed using a Quantstudio 3 (Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... We isolated the transposed and adaptor-ligated genomic DNA from the reaction with the Zymo DNA Clean and Concentrator--5 Kit (Zymo #D4014) and then amplified the libraries using the custom primers described in Supplemental Table 4 (originally designed by the William Greenleaf lab at Stanford University) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Genomic DNA was extracted from the hippocampus using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, Irvine, CA, United States) and checked for purity and quantity using a NanoDrop spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... DNA extraction was done using a Quick-DNATM fungal/bacterial miniprep kit following the manufacturer’s protocol (Zymo Research, Irvine, CA, USA). The extracted DNA quality was checked by loading 5 µl DNA + 2 µl 6× loading dye with gel red on a 1% agarose gel and separated at 100 V for 40 min ...
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bioRxiv - Genetics 2024Quote: ... followed by resuspension of ∼200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
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bioRxiv - Microbiology 2024Quote: Sterivex filters were extracted in-house at NOAA’s Atlantic Oceanographic and Meteorological Laboratory (AOML) using the ZymoBIOMICS 96 DNA/RNA MagBead kit (Zymo; CAT# D4308), with modifications for in-cartridge bead beating as described in (110) ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was prepared from 1×106 cells of HAP1 and HELLS KO using Direct-zol RNA Microprep kit (Zymo Research). The RNA was checked for quality using the Agilent BioAnalzyer 2100 ...
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bioRxiv - Plant Biology 2024Quote: ... Final GBKT7 plasmids was transformed into the yeast strain Y2HGold and transformants selected on SD medium lacking tryptophan (Trp) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). Quantitative transactivation tests were performed as previously described (Ref) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from TRAP and input samples with TriZol and the Direct-Zol RNA MicroPrep Kit (Zymo Research, Orange, CA).
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bioRxiv - Microbiology 2024Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Molecular Biology 2024Quote: ... A band from 250-400bp was cut and DNA was extracted in 150 μl of ddH2O using the Gel DNA Recovery Kit (Zymo D4007).
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bioRxiv - Biophysics 2024Quote: ... DNase I digestion was stopped by adding EDTA to 15 mM (final) and RNA was extracted with RNA Clean and Concentrator Kit (Zymo Research). Eluted RNA was combined with Qubit HS RNA Reagent and Qubit HS RNA Buffer and measured using a Qubit fluorometer using the HS RNA protocol after RNA standardization (Life Technologies) ...
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bioRxiv - Genomics 2024Quote: Genomic DNA from placental tissues was isolated using a Quick-DNA mini prep plus kit according to the manufacturer’s instructions (Zymo Research D4068). LINE-1 methylation levels were quantified using an ELISA-based Global DNA Methylation Assay LINE-1 kit ...
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bioRxiv - Genomics 2024Quote: ... one additional library per sample was constructed using the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, Irvine, CA, USA). Each library type was pooled and 150 bp paired-end reads generated on an Illumina NextSeq 550 instrument at the University of Saskatchewan Next-Generation Sequencing Facility ...
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bioRxiv - Genetics 2024Quote: ... cerevisiae transformants were plated on YNB plates lacking uracil and plasmid DNA was recovered from yeast transformant cells using the Zymoprep Yeast Plasmid Miniprep II Kit (D2004, Zymo Research), then transformed into DH5α Escherichia coli cells by electroporation ...
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bioRxiv - Synthetic Biology 2024Quote: ... gDNA was extracted from spores collected from colonies on solid plate or mycelium from bioreactor experiments using the Quick-DNA Fungal/Bacterial Miniprep Kit (ZYMO RESEARCH). For Southern-Blot ...
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bioRxiv - Synthetic Biology 2024Quote: ... Multiple PCR reactions of each fragment were pooled and purified by gel purification using the ZymocleanTM Gel DNA Recovery Kit (ZYMO RESEARCH) and used for transformation.
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bioRxiv - Bioengineering 2024Quote: ... The uxuB leader and first 100 bases of coding sequence was transcribed using the same in vitro transcription methodology described in “In vitro RNA-protein binding assays’’ and purified using the Zymo RNA Clean and Concentrator spin-column extraction kit (Zymo Research). RNA concentration was then quantified using the NanoDrop® ND-1000 UV-Vis Spectrophotometer ...
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bioRxiv - Synthetic Biology 2024Quote: All plasmids were prepared from 25-50 mL overnight cultures grown at 37°C in LB with appropriate antibiotics and purified using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research). Plasmids with methylated HIP1 sites were miniprepped from E ...
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bioRxiv - Cancer Biology 2024Quote: ... was incubated for 30 minutes on a rotating orbital.DNA recovered from dynabeads was purified with a DNA clean and Concentrator-5 kit (Zymo Research, D4013). Amplification of the recovered DNA was carried out using a whole genome amplification kit (WGA2 ...
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bioRxiv - Bioengineering 2024Quote: ... complete medium was transformed with the yeast-display plasmid pCTCON2 (24) using the Frozen E-Z Yeast Transformation II kit (Zymo Research) according to manufacturer protocols ...
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA from one full-scale lentiviral infection replicate was extracted using Quick-DNA Midiprep Plus kit and protocol (Zymo, D4075). An Alt-R S.p ...
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bioRxiv - Cancer Biology 2024Quote: ... they were washed twice in ice-cold DPBS and then collected in Tri-reagent for storage at -80°C until the bulk RNA was extracted using Direct-Zol RNA Miniprep Kit (Cat#R2050, Zymo Research).
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted from HG-cultured β-cell lines and processed using the EZ-DNA Methylation Direct Kit (Zymo Research). The treated DNA was amplified by PCR and purified with a gel extraction kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... Oligonucleotides were 5ʹ-end labeled with T4 polynucleotide kinase and [γ32P]-ATP and then purified using the Oligo Clean and Concentrator Kit (Zymo Research). Recombinant protein and oligonucleotides were incubated in 20 µl of EMSA buffer (50 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA extraction from drug-resistant cells was performed using the Zymo QuickDNA™ Midiprep Plus Kit (Zymo Research, Cat. D4068), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA (250 to 500 ng) of the FD and FT cells was converted by sodium bisulfite using the EZ DNA Methylation-Lightning kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... E.coli stocks were expanded in LB with ampicillin overnight and plasmids were isolated with ZymoPURE II Plasmid kit (Zymo Research #D4203). Lentiviral particles were prepared with Lenti X 293T cell using VSV-G pseudotyped lentiviral packaging (TakaraBio ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of DNA from the validation cohort of 20 patients (20 adenoma and 20 nontumor tissue samples) was processed with the EZ DNA Methylation-Gold Kit following the standard protocol (Zymo Research). Bisulfite conversion was followed by amplification of the target CpGs ...