Labshake search
Citations for Zymo Research :
5551 - 5600 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: RNA isolation of the FACS-purified GFP+ Treg cells was performed by the Quick-RNA Microprep kit following the manufacturer’s protocol (Zymo Research). RNA quality control was performed with the Agilent 4200 Tape Station system and only RNAs having a RIN >8 were used for library preparation ...
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bioRxiv - Microbiology 2022Quote: ... using a modified version of the Quick DNA/RNA pathogen magbead 96 extraction kit (Zymo Research Corp., Irvine, CA, USA) to automate total nucleic acid extraction and DNase treatment ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from the pelleted cells using a Quick RNA Fungal/Bacterial Microprep kit (Zymo Research, Irvine, CA, USA). The cells were mechanically lysed with Mini-Beadbeater-96 (Biospec Products ...
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bioRxiv - Microbiology 2022Quote: 74μL of ultrapure water was added to the remaining sample (to make up to 100μL total volume) and each reaction was then purified using the Zymo Genomic Clean and Concentrate kit (Zymo D4011). The DNA was eluted with 10mM Tris buffer heated to 63°C and incubated for a few minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR reactions from the same time point were then pooled together and purified using a spin column DNA clean and concentrate kit (Zymo). To prevent oversaturation of the spin column ...
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bioRxiv - Pathology 2022Quote: RNA was extracted and cDNA was synthesized according to the manufactures’ manuals of Quick-RNA Miniprep kit (Zymo Research, R1054) and High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from young leaves and floral buds using the Zyppy DNeasy Extraction kit (Zymo Research, CA, USA) according to the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Relative quantification of 5-methylcytosine (5-mC) over time was determined using the 5-mC DNA ELISA Kit (Zymo Research), using 100 ng of genomic DNA per sample as input ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... we used a modified protocol of the Direct-zol RNA extraction kit (Catalog No. R2501, Zymo Scientific, Irvine, CA, USA) along with TriSure reagent (Catalog No ...
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bioRxiv - Microbiology 2023Quote: ... and total RNA was extracted from individual samples using a Zymo Quick-RNA Tissue/Insect Microprep Kit (Zymo Research, #R2030). RNA was eluted into 50 μL of RNase-free water and stored at −80 °C prior to reverse transcription ...
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bioRxiv - Microbiology 2022Quote: ... The 190 samples were extracted in the summer of 2021 using ZymoBionics DNA miniprep Kit (Zymo Research, Irvine, California, USA). We used the standard extraction protocol with modifications ...
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bioRxiv - Microbiology 2022Quote: ... and gDNA was extracted from cell pellets by the CTAB-lysozyme method (80) and cleaned using the Genomic DNA clean and concentrator kit (Zymo) before submitting to UMGC for Tn-seq library preparation and Illumina sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... Ligated RNA samples with unique barcodes were pooled and cleaned up using the Oligo Clean & Concentrator kit (Zymo Research, #D4060). All samples were next reverse transcribed by using Superscript III (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Linearized plasmid DNAs and RNA bait DNAs were kit purified prior to IVTT (DNA Clean and Concentrator; Zymo Research, D4004). All DNAs were eluted into Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2022Quote: RNA from resting B cells isolated from three wild-type animals was isolated using Direct-zol RNA Miniprep Kit (Zymo). Libraries were constructed using the Illumina TruSeq Stranded Total RNA Library and sequenced on NextSeq2000 with PE100 sequencing ...
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bioRxiv - Microbiology 2022Quote: ... erythrocytes were lysed in 0.15% saponin before RNA was extracted from parasite pellets using a Direct-zol RNA Miniprep Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and total RNA was isolated using a Direct-zol RNA Miniprep Plus Kit following the manufacturer’s instructions (ZYMO RESEARCH; USA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted by phenol/chloroform and purified through the RNA Clean and Concentrator-25 kit (Zymo Research, R1018), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed and corrected recombinant plasmids were digested with Not I for linearization and then transformed into Po1fΔKu70 using the Frozen-EZ yeast transformation II Kit (Zymo Research). Transformants were screened on YNB plates supplemented with auxotrophic supplements containing appropriate amino acids and placed in an incubator at 30 °C for 2-3 day ...
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bioRxiv - Synthetic Biology 2024Quote: DNA templates for expression in PUREfrex were prepared either in plasmid form using ZymoPURE II Plasmid Midiprep Kit (Zymo Research) or as linear expression templates (LETs) ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA samples were prepared for targeted sequencing with the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Primers were custom designed by Zymo Research to ensure best coverage of the 16S gene and high sensitivity - Quick-16S™ Primer Set V1-V3 (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from various developmental stages and adult tissues using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and further purified with the RNA clean and concentrator-5 kit (Zymo), following the provided instructions ...
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bioRxiv - Microbiology 2023Quote: ... The negative controls had no or extremely low DNA concentrations to proceed to library preparation as determined by RT-PCR using the Femto Bacterial DNA Quantification Kit from Zymo and were therefore excluded from further analysis (n =10 ...
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bioRxiv - Physiology 2024Quote: ... The VAT was weighed prior to use and 50 ug of tissue and DNA was extracted using the Quick-DNATM Miniprep Plus Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The size of the PCR products was verified by agarose gel electrophoresis and purified by using the Zyppy extraction kit (Zymo). The amplified PCR products were digested with BamHI and XhoI and ligated with similarly digested pCTX-1-lux ...
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bioRxiv - Genetics 2024Quote: ... The library was transformed into yWS2542 (ram11 ydj11) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA) per the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 ng DNA were extracted after MTAC induction and bisulfite-converted using EZ DNA Methylation-Gold Kit (Zymo Research, D5005). PCR amplification was performed to convert ssDNA to dsDNA using primers designed for a region spanning the MATa and the backbone of LacO plasmid used for integration (Table S2) ...
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bioRxiv - Microbiology 2024Quote: ... The tissues were homogenized by bead beating and viral RNA was extracted using the RxnDirect-zol RNA Miniprep Plus kit (Zymo). For all samples the viral RNA was quantified by qRT-PCR as described above.
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bioRxiv - Genomics 2024Quote: Genomic DNA (gDNA) of S1 and S2 was extracted using the Quick-DNA fungal/Bacterial Miniprep Kit (Zymo Research, USA) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... These spike-in samples then undergone bisulfite conversion and purification by EZ DNA Methylation-Gold Kit (Zymo Research, D5006, USA). DNA library was prepared from bisulfite-converted DNA samples using xGen Methyl-Seq DNA Library Prep Kit (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2023Quote: DNA extraction from whole input cells and purified nucleoli was performed using a Quick-DNA Universal Kit (Zymo Research, CA). DNA concentration was measured using a Qubit dsDNA HS Assay kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and 10 conventional + Abx individuals was extracted with the Quick-DNA Tissue/Insect Microprep Kit (Zymo Research, Irvine, CA, USA), according to the manufacturer instructions and eluted in 30-40 µL elution buffer ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were cleaned up with RNA Clean and Concentrator 100 columns (Zymo, referred to as “kit-purified” in text). Purity was assessed using a 6% Tris-Borate-Urea gel (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... The cells were scraped into solution and total RNA was purified using Zymo Quick-RNA Miniprep kit (Zymo Research, R1054). Of the isolated total RNA ...
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bioRxiv - Plant Biology 2024Quote: ... we sorted into 20 μl of RNA-Later then used the magnetic bead based Direct-zol-96 MagBead RNA-Isolation kit (Zymo) to isolate RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from MV4;11 Cas9 + GeCKOv2 cells collected before and following sustained C6-treatment using the Quick-gDNA MidiPrep Kit (Zymo Research) per manufacturer’s directions ...
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bioRxiv - Cell Biology 2024Quote: ... when RNA was further purified using the RNA Clean & Concentrator kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: ... A single colony was picked and grown in terrific broth (TB) to an optical density (OD) of 0.5.RNA isolation was performed using Direct-zol RNA Miniprep Kit (Zymo Research). RNA was prepared for small RNA sequencing according to the following protocol ...
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The supernatant was carefully transferred to a fresh tube and further processed using the Direct-Zol RNA Microprep Kit (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: RNA from the same sample material was extracted using the Quick-RNA Fungal/Bacterial miniprep kit (Zymo Research, CA, USA) using the manufacturer’s protocol and eluted in 25 µl of DNase/RNase free water ...
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bioRxiv - Genomics 2024Quote: ... The recovered rRNA depleted total RNA was further cleaned up and concentrated with the RNA Clean & Concentrator kit (Zymo, R1016).
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bioRxiv - Genomics 2024Quote: ... Eluted DNA was treated with Proteinase K at 55ºC for 30 min and then purified with a ChIP DNA Clean and Concentrator kit (Zymo Research) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: CP-KO RNA was extracted from 15 3h PBF guts with the Direct-zol RNA Mini-prep kit (Zymo Research) including on-column DNase treatment and transcribed into cDNA with the qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was extracted using hot acid phenol method followed by purification using Direct-zol RNA Miniprep Kit (Zymo Research). The RNA was treated with DNaseI ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from 3 biological replicates of 100mg of frozen tissue of each accession using the Quick-DNA/RNA Miniprep kit (Zymo) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
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bioRxiv - Microbiology 2023Quote: Cell pellets were re-suspended in TRIzol reagent and subjected to three cycles of 1min bead-beating (0.5mm silica/zirconia beads) followed by RNA extraction using the Direct-zol RNA Kit (Zymo research) according to the manufactures’ protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and RNA was isolated by miRNeasy Mini Kit solutions (Qiagen217004) and Zymo-Spin IIICG Columns (Zymo Research C1006-50-G) following the manufacturer’s instructions.