Labshake search
Citations for Zymo Research :
5301 - 5350 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... RNA was purified using an RNA Concentration and Clean-Up kit as per manufacturer’s instruction (Zymo, Cambridge Biosciences, R1013). The integrity and quantity of total RNA were determined using Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genomics 2024Quote: ... RNA extraction for sequencing was carried out using the Zymo Quick-RNA Viral Kit (Zymo Research, Orange, CA, USA). cDNA synthesis and sequencing library preparation were performed following the ARTIC v4 protocol ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted and bisulphite converted using the EZ-96 DNA Methylation Kit (Zymo Research, Orange, CA, USA). Subsequent methylation analysis was performed on an Illumina (San Diego ...
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bioRxiv - Neuroscience 2024Quote: Snap-frozen brain tissues were processed for RNA isolation using the RNA Miniprep Plus Kit (Zymo Research, Catalog #R1055) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... recovery of laccase-containing plasmids from these cultures was performed using Zymoprep Yeast Plasmid Miniprep Kit (Zymo research, #D2001) regarding the manufactureŕs specifications ...
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bioRxiv - Cancer Biology 2024Quote: ... Microbial DNA from cell pellets and tissue samples was extracted with the ZymoBIOMICS DNA miniprep kit (Zymo Research, D4300), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The final purification of amplified DNA was performed with the DNA clean and Concentrator-5 kit (Zymo Research, D4013) and quantified using NanoDrop one (260nm) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from isolated mitochondria using the Zymo Direct-Zol Micro Prep kit (R2060, Zymo, CA, USA) with on-column DNase-I digest (E1010 ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 µL of the aqueous layer was carefully retrieved and input into the RNA Clean and Concentrator Kit (Zymo) for further RNA purification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products for all primer combinations were gel extracted and purified using the Zymoclean Gel DNA Recovery Kit (Zymo) for verification by Sanger sequencing.
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products of approximately the correct size were gel extracted and purified using Zymoclean Gel DNA Recovery Kit (Zymo) for verification by Sanger sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction was performed on the aqueous phase using Direct-zol RNA Miniprep kit (Zymo Research Corporation, California, USA). 1 µg of total RNA was used for cDNA synthesis using High-Capacity cDNA Reverse Transcription Kit while following manufacturer’s protocol (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... for 5 minutes and then RNA was purified using Direct-zol RNA Miniprep kit according to the manufacturer’s instructions (Zymo). RNA was reverse transcribed using UltraScript cDNA synthesis kit (PCR Biosystems) ...
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bioRxiv - Evolutionary Biology 2024Quote: Gut and skin microbiota samples were extracted using the ZymoBIOMICS®-96 MagBead DNA Kit (Zymo Research, Irvine, CA) using an automated platform.
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA from the aqueous phase-ethanol mix was subsequently extracted using RNA Clean & Concentrator-25 kit (Zymo Research) following the manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: RNA was extracted from a total of 100 mg of ground frozen shoot tissue with Direct-zol kit (Zymo Research) according to manufacturer’s protocol with in-column DNAse treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... Eluates were then concentrated in a SpeedVac to ∼100 µL and purified with the DNA Clean and Concentrator Kit (Zymo). Libraries were sequenced on a HiSeq 2000 at the BioMicroCenter Core Facility at MIT with 50 nt read length.
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was extracted from no treatment and SHX-treated flash frozen cells using the Quick RNA extraction kit (Zymo) following the standard protocol ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted using Direct-zol RNA MicroPrep kit according to manufacturer’s instructions (cat no. R2062, Zymo Research, Irvine CA). Purified RNA samples underwent fragment analysis at the MIT BioMicro Center ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from cells lysed with Trizol using the Direct-zol RNA Miniprep Plus Kit (Zymo Research, R2072). 1 µg of total RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... 500 ng of genomic DNA were bisulfite converted using the EZ-96 DNA methylation Gold Kit (Zymo research, Irvine, USA) according to the kit’s manual ...
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bioRxiv - Genetics 2021Quote: IL2 and CD20 constructs were created through PCR amplification from cDNA and genomic DNA respectively.PCR products were purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research) and cloned into the pEntryD-Topo vector ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA from strains that had at least an IC50 shift of 1.5-fold was extracted using the YeaStar Genomic DNA kit (cat. No D2002, ZYMO Research).
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted from embryos and three-week old plants using Quick-DNA™ Micro prep Kit (Zymo D3020) according to the recommendations of the manufacturer.
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bioRxiv - Evolutionary Biology 2021Quote: ... bacterial colonies were selected and grown in overnight cultures for plasmid purification using the Zyppy Plasmid Miniprep kit (Zymo Research). Sanger sequencing of clones was performed using M13F and M13R primers ...
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was extracted and genomic DNA was removed using the 96-well Direct-zol MagBead RNA Kit (Zymo Research). RNA concentrations and contamination were determined spectrophotometrically using a Nanodrop ...
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bioRxiv - Developmental Biology 2020Quote: Combinations of the AD or BD vectors were co-transformed into yeast using the Frozen-EZ II kit from Zymo Research and using empty vectors as negative controls ...
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bioRxiv - Developmental Biology 2020Quote: ... and stored in −80°C until extraction of total RNA extraction using the Direct-zol™ RNA kit (Zymo Research). The optical density value of total RNA was measured using the NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... 1ng of each library constructed was used in a polymerase chain reaction with primers flanking the allele and the barcode to generate fragments which were subsequently gel verified and extracted using Zymo gel extraction kit (Zymo). 25ng of the purified product was subjected to self-ligation at 16 °C overnight in a total volume of 50uls and column purified using Qiagen (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was isolated by phenol-chloroform extraction followed by purification using the Genomic DNA Cleanup and Concentration kit from Zymo Research (catalogue number D4065) ...
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bioRxiv - Genomics 2020Quote: ... the total RNA was extracted from active and tun samples (3 replicates) using Direct-Zol RNA MicroPrep Kits (Zymo Research), and the sequencing library was constructed using SMARTer v4 Ultra Low Input RNA Kit for Sequencing (Clonetech ...
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bioRxiv - Molecular Biology 2022Quote: ... We then digested the template DNA using TurboDNase at a concentration of 0.1 U/μl in 200 μl and cleaned up the reaction using the RNA Clean & Concentrator-25 kit (Zymo Research). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... 740727.250) was from BIOKE (Leiden, The Netherlands). DNA purification was achieved using DNA Clean & Concentrator kit (Cat. D4029) from Zymo Research (California ...
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bioRxiv - Genomics 2022Quote: ... FCG hippocampal DNA (200 ng) was bisulfite converted with the EZ DNA Methylation-Lightning Kit (#D5030T; Zymo Research, Irvine, CA), according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids with the A/B standards were then extracted and purified using the ZymoPURE Plasmid Miniprep Kit (Zymo Research), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The pooled PCR products for each sample were cleaned up using a column-based kit (Zymo DNA Clean and concentrator), eluted in 17 μl of nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... high molecular weight genomic DNA (1µg) was subjected to bisulfite conversion using the EZ DNA Methylation-Direct kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... a further round of purification can be performed with the Zymo Oligo Clean and Concentrate spin-column kit (Zymo D4060). Labeled probe concentration was measured via NanoDrop OneC UV-Vis Spectrophotometer (Thermo Scientific 840-274200).
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bioRxiv - Molecular Biology 2020Quote: ... The enriched RNA and input total RNA from 5% of lysate were purified using Direct-zol RNA Purification Kit (Zymo). Target genes in input total RNA and enriched RNA were quantified by qRT-PCR using SuperScript III Platinum One-Step qRT-PCR Kit (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: Total RNAs were extracted from BMDMs (male mice: 4 Cre+ and 4 Cre−) using the Quick-RNA miniprep plus kit (Zymo). With a minimum of 300 ng input RNA ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from ∼25 dissected neonate midguts using Direct-zol RNA Mini Prep kit (Zymo Research, Irving, CA) with DNase treatment (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed in Tri-reagent and total RNA was isolated using the Direct-zol RNA mini-prep kit (Zymo). For spike-in ...
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bioRxiv - Genomics 2020Quote: ... DNA was sheared to an average size of 200 bp and bisulfite converted with the EZ DNA methylation kit (Zymo). Paired end reads (100 ...
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bioRxiv - Cancer Biology 2020Quote: ... cfDNA was isolated from 0.5 - 10 ml of plasma using the Quick-cfDNA Serum & Plasma Kit centrifugation protocol according to the manufacturer’s protocol (Zymo Research). cfDNA was eluted twice in the provided elution buffer or in MilliQ and subsequently stored at −80°C ...
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bioRxiv - Genetics 2021Quote: ... 100µl of the gDNA solution was purified using the Zymo Genomic DNA Clean and Concentrator –10 kit (Zymo Research #ZD4010) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from tail snips above was cleaned using Genomic DNA Clean and Concentrator kit (Zymo Research, Irvine, CA). qPCR was performed with CD45 and RIP-DTR forward and reverse primers used above and SYBR green master mix (Bimake ...
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bioRxiv - Genetics 2020Quote: ... Digested plasmids were run in a 1.2% agarose gel and the ACE2 band was excised and purified using the Zymo Gel Extraction kit (Zymo Research). Purified DNA was submitted to the University of Wisconsin-Madison Biotechnology Center DNA Sequencing Facility for analysis.
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bioRxiv - Genomics 2020Quote: ... 200 ng of extracted genomic DNA was subsequently bisulfite-converted with the EZ DNA Methylation Kit (Zymo Research, Irvine, USA).
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bioRxiv - Molecular Biology 2020Quote: ... The ribosome pellet was resuspended in a solubilization buffer containing 0.5% SDS and 1mM EDTA and TRIzol before RNA was extracted using the Direct-zol kit (Zymo Research). RNA was separated on a 15% TBE-Urea gel before RNA between 17nt and 34nt were gel extracted ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We pooled up to three extractions per sample using the Genomic DNA clean & Concentrator kit (Zymo Research, Irvine, CA, USA), following the supplied protocol ...