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Citations for Electron Microscopy Sciences :
351 - 400 of 458 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The testes were extracted and fixed for two days at 4 °C in 4 % PFA (Electron Microscopy Sciences, Hatfield, PA ,USA). Samples were then washed with PBS for at least two days ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were submerged in 1.5% uranyl acetate in absolute methanol at -90 °C for 2 days and infiltrated with HM20 Lowicryl resin (Electron Microscopy Sciences) over 2 days at -45 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Cell Biology 2022Quote: ... falciparum 3D7 culture was pelleted by centrifugation (1500g, 3 min, RT) and resuspended in freshly prepared PBS containing 4% EM-grade para-formaldehyde (PFA, Electron Microscopy Sciences) and 0.1% EM-grade GA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The buffer was removed and samples stained with post-fixative for 3 hours (Osmium tetroxide, Ted Pella; Tannic Acid, Electron Microscopy Sciences). After washing with distilled water ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Immunology 2024Quote: ... At -60 °C samples were infiltrated with increasing concentrations (30, 60, and 100% for 1 h each) of Lowicryl (3:1 K11M/HM20 mixture; Electron Microscopy Sciences). After 5 h of 100% Lowicryl infiltration ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of this sample at 2 µg Chl mL−1 was applied to a glow discharged (60 s, 25 mA) 400 mesh Cu grid with 5-6 nm formvar with 3-4 nm carbon (Electron Microscopy Sciences). The sample was negatively stained with 2% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... amylovora strain Ea110 were collected at 3 dpi and fixed in paraformaldehyde/ glutaraldehyde (2.5% of each compound in 0.1 M sodium cacodylate buffer) (Electron Microscopy Sciences, Hatfield, PA) at 25 C overnight ...
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bioRxiv - Biochemistry 2024Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: 4 μL of the GUV COPII BR was applied to negatively glow-discharged C-flat holey carbon coated gold grids (CF-4/1-4AU, Electron Microscopy Sciences), blotted from both sides (60 seconds pre-blot wait ...
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bioRxiv - Neuroscience 2020Quote: ... rats were deeply anesthetized with sodium pentobarbital (180 mg/kg; Fatal-Plus C IIN, Vortech Pharmaceuticals, Dearborn, MI) and perfused with paraformaldehyde (#15710, Electron Microscopy Sciences (EMS), Hatfield ...
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bioRxiv - Microbiology 2022Quote: Cells were seeded onto coverslips for immunofluorescence experiments and fixed for 10 mins at 37 °C in 4% (v/v) paraformaldehyde (Electron Microscopy Sciences). Samples were permeabilized with 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... The 40 μm cryosections were cut at -18°C and placed over glass slides treated with tissue capture pen (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Neuroscience 2021Quote: ... samples were placed on a Durcupan block with a flat surface and covered with ACLAR® 33 C Film (Electron Microscopy Science). Samples were let polymerize for 72 h at 60 °C.
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bioRxiv - Cell Biology 2022Quote: ... 3.5 µl of each sample was applied to the plasma-cleaned C-flat grids (Electron Microscopy Sciences, CF-1.2/1.3-4Au-50), pre-blotted and blotted with filter paper (Whatman 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Biophysics 2022Quote: ... Samples of cores undergoing ERT for 4 h at 37 °C were mixed with equal volumes of 10-nm BSA Gold Tracer (Electron Microscopy Sciences), and 3.5 uL aliquots were applied onto glow-discharged Quantifoil grids (Electron Microscopy Sciences) ...
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bioRxiv - Plant Biology 2021Quote: ... LPI-1 from growth chamber plants or young leaves of tissue culture plants were processed for primary fixation at 25°C in 2 % glutaraldehyde (Electron Microscopy Sciences, EMS) prepared with Sorensen’s Phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Neuroscience 2023Quote: ... At −50 °C samples were washed four times in ethanol for 30 minutes each before gradual infiltration with HM20 resin (Electron Microscopy Sciences). Polymerization of the resin was done with a UV light source and was carried out at −50 °C for 48 hours followed by post-polymerization at room temperature for 2 days.
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bioRxiv - Molecular Biology 2022Quote: ... 1 ug of RNA was denatured at 95°C for 1 minute and subsequently refolded by incubating in 340mM sodium cacodylate buffer (Electron Microscopy Sciences) and 5mM MgCl2 for 20 minutes at 37°C in a thermomixer ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... The fungal samples were fixed for 12 h at 4 °C using 2.5% glutaraldehyde and 2 % paraformaldehyde (Electron Microscopy Sciences, Hartfield, PA) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were washed with pre-warmed (37°C) PBS and permeabilized for 2 minutes using 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37 °C ...
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bioRxiv - Biophysics 2022Quote: ... 3.5 μL of purified virions at 1 × 1010 PFU mL-1 was applied to a 400-mesh C-flat holey carbon-coated grid (Electron Microscopy Sciences) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.4 buffer for P116 without the N-terminal domain (246–818)) was applied to a 45 s glow- discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2024Quote: Cells grown on 22x22 mm glass coverslips were fixed for 20 min at room temperature (25°C) with 4% PFA (Electron Microscopy Sciences) diluted in PBS and stored at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Three to four microliters of rsVLP or rsVLP-compound-GSH mixture were applied to either glow-discharged C-flatTM holey carbon copper grids (product No. CF312, Electron Microscopy Sciences) for the PV1-SC6b yeast sample or Lacey carbon copper grids with an ultra-thin carbon support film (product No ...
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bioRxiv - Biophysics 2024Quote: ... 200 mM NaCl and 0 – 0.01% LMNG or 1 mM CHAPSO) was applied to a 45 s glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA) or R1.2/1.3 300 copper mesh quantifoil grid (Quantifoil Micro Tools ...