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Citations for Electron Microscopy Sciences :
251 - 300 of 458 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The supernatant was discarded and the pellet was kept at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science) before analysis.
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently incubated with rotation at 4 °C first in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Cat: 15710-S) for 24 hours and then in 30% (w/v ...
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bioRxiv - Genetics 2023Quote: ... were imbibed overnight in water at 4 °C and then incubated for 12 h in 50 mL of 0.25% ethyl methane sulfonate (EMS). The EMS was subsequently washed several times with water ...
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bioRxiv - Cell Biology 2024Quote: ... The tissue blocks were rinsed three times with water and en bloc stained overnight at 4°C with an aqueous solution of 0.5% w/v uranyl acetate (EMS). The tissue blocks were dehydrated in ethanol ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated eyes were fixed for 2 hours at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1× PBS ...
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bioRxiv - Microbiology 2021Quote: ... was performed by placing 5 μL drops of the bacteria suspension for 3 minutes directly on glow discharged carbon coated grids (EMS). The grids were then washed with two drops of 2% aqueous uranyl acetate ...
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bioRxiv - Bioengineering 2021Quote: ... the 3D printed constructs were kept in culture for 3 weeks before fixing using paraformaldehyde (4%, Electron Microscopy Sciences, USA) for 45 minutes at RT ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Cell Biology 2021Quote: Organoids were fixed in 3% glutaraldehyde and 1% paraformaldehyde in 0.08 M sodium acodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 3 µL aliquots of purified ex vivo Enas were deposited on Formvr/Carbon grids (400 Mesh, Cu; Electron Microscopy Sciences), washed with 20 μL 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed three times in sterile PBS and fixed in 3% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 min at room temperature (RT) ...
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bioRxiv - Physiology 2022Quote: ... they were rinsed 3 times 5 minutes in PB buffer and then postfixed by a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were rinsed 3 × 5 min in PB and post-fixed in a fresh mixture of osmium tetroxide 1% (EMS) with 1.5% potassium ferrocyanide in PB buffer for 2 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 μl was applied to a glow discharged (PELCO easiGlow) carbon-coated 300 mesh copper grid (Electron Microscopy Sciences). Grids were stained with 2% uranyl acetate and allowed to dry ...
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bioRxiv - Cell Biology 2024Quote: ... were washed with Phosphate Buffer (PB) 0.1 M pH 7.2 (NaH2PO4·H2O and Na2HPO4) and fixed with 3% glutaraldehyde (Electron Microscopy Sciences) for 10 min at 37°C and 2 h at RT ...
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bioRxiv - Physiology 2024Quote: Isolated TALs and DCTs were embedded into 1% agarose and fixed in 3% formaldehyde/glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) for 24 hours ...
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bioRxiv - Genomics 2024Quote: Liver tissue sections (3-5 mm thickness) were fixed by submerging in fresh 4% v/v paraformaldehyde (PFA; EMS 15710) in 1X Phosphate Buffered Saline (PBS ...
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Immunology 2023Quote: ... in HBS buffer were mixed and incubated for 30 minutes on ice prior to application of 3 uL to Quantifoil R 2/2 plunge freezing grids (EMS) that were glow discharged (negative charge ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) and fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) then fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... 2.5 μl of ∼3 mg/ml BmrCD was placed onto the Quantifoil UltrAuFoil grids (R1.2/1.3, 300 mesh, Electron Microscopy Sciences) which were glow discharged for 20 sec at 25 mA ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Biophysics 2024Quote: ... the flame sealed end of the tubing was immediately dipped into a parlodion solution (∼3 cm2 parlodion, Electron Microscopy Sciences [Hatfield ...
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2024Quote: ... This cell suspension (3 µl) was applied to Cflat-2/1 200 mesh copper or gold grids (Electron Microscopy Sciences) that were glow discharged for 30 seconds at 15 mA ...
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bioRxiv - Biophysics 2024Quote: ... were diluted to[9 mg/ml and aliquots (3[μl) were applied to GF-0.6/1.0 300 Au-flat (Electron Microscopy Sciences). The cryo-EM grids were prepared using Thermo Fisher Vitrobot Mark IV at 8[°C and 100% humidity (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of F-actin diluted to 0.6 μM in KMEI + NP40 was applied to a glow discharged C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica GP plunge freezer operating at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... 3.5 µl of the complex solution were deposited onto a C-flat 1.2/1.3-3C holey carbon copper grid (Electron Microscopy Sciences) that had been freshly glow-discharged for 1 min at 20 mA using a PELCO easiGLOW (Ted Pella) ...
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bioRxiv - Cell Biology 2021Quote: ... then rinsed in 100% acetone and infiltrated over 24 h at −600°C with Lowicryl HM20 resin (Electron Microscopy Science). Samples were placed in gelatin capsules and UV hardened at −450°C for 48 h ...
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bioRxiv - Biochemistry 2022Quote: A Gatan Solarus Model 950 advanced plasma system was used to clean C-Flat holey carbon grids (hole size: 1.2 µm; spacing: 1.3 µm; mesh: 400; Electron Microscopy Sciences). Settings used for the plasma cleaning were ‘Cleaning time’ ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of 10 μM GMPCPP-MT (polymerised as described above) were applied to a glow-discharged C-flat 2/2-4C grid (EMS) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... 3 μl of solution was applied to a plasma-cleaned C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica EM GP plunge freezer operating at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μL of NT-NTR1-Gαi1β1γ1-cND at a concentration between 1.5 mg/mL to 1.7 mg/mL was applied onto glow discharged C-flat holy carbon grids (R1.2/1.3, 400 mesh copper, Electron Microscopy Sciences) or Quantifoil holy carbon grids (R1.2/1.3 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain and pituitary were taken and fixated overnight at 4 °C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, Pennsylvania) diluted with phosphate buffered saline with Tween (PBST ...
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bioRxiv - Immunology 2020Quote: ... were loaded onto a glow-discharged C-flat grid (CF-1.2/1.3 400-mesh copper-supported holey carbon, Electron Microscopy Sciences), blotted for 4–5 s under 100% humidity at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... we immersed slides from −80 °C instantly into freshly made 4 % PFA (10mL vials of 20 % PFA; Electron Microscopy Sciences) in PBS for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... was subsequently applied on a glow-discharged C-flat CF-1.2/1.3 400 mesh holey carbon grids (Electron Microscopy Sciences), blotted with Vitrobot Mark IV (FEI) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were fixed overnight at 4°C in freshly prepared phosphate buffer (0.1 M, pH 7.4) containing 4% paraformaldehyde (15700, Electron Microscopy Sciences) and 0.2% glutaraldehyde (16200 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Biochemistry 2023Quote: Three microliters of the freshly purified complex were applied to a plasma-cleaned C-flat holy carbon grid (1.2/1.3, 400 mesh, Electron Microscopy Sciences) with 20 s glow discharge using air and flash-frozen in liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Three washes with cold acetone were carried out at 0°C and samples were embedded in Embed-812 (EMS, USA). After polymerization of the resin at 60 °C for 36 h ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then fixed during confinement for 60 min at 37°C with 4% paraformaldehyde (PFA; Electron Microscopy Science,15710) in CB to allow fixative to penetrate the PDMS cell confiner ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were removed from the-80°C freezer and placed immediately into ice cold 4% PFA (Electron Microscopy Sciences, #15714)/PBS (MP Biomedicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...