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Citations for Electron Microscopy Sciences :
301 - 350 of 458 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Microbiology 2023Quote: ... then rinsed in 100% acetone and infiltrated over 24 h to -45°C with Lowicryl HM20 resin (Electron Microscopy Science). Samples were placed in gelatin capsules and UV hardened at -45°C for 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... heated in Volvic mineral water 5 min at 70°C to extend the tardigrade body and directly fixed with 4% formalin (15686, EMS) in 1X PBS-1% Triton-X100 (PBS-Tx ...
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bioRxiv - Microbiology 2024Quote: ... Carbon-coated copper grids (200 mesh) and C-flat cryo-EM grids were purchased from Electron Microscopy Sciences (Hatfield, PA). Lacey carbon cryo grids and 2% (w/w ...
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bioRxiv - Physiology 2024Quote: ... slides were heated for 30 minutes at 65°C followed by submersion in Histoclear II (Electron Microscopy Sciences 64111-04) twice for five minutes ...
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bioRxiv - Molecular Biology 2024Quote: Enucleated adult eyes were fixed for 1 hour at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Science, Hatfield, PA) prepared in phosphate-buffered saline (1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... animals were transcardially perfused with 20 mL of ∼37°C PBS 0.1M followed by 20 mL of ice-cold 4% paraformaldehyde (EMS, 15714S) in PBS 0.1M ...
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bioRxiv - Developmental Biology 2024Quote: Larvae were fixed for 2 hours at room temperature or overnight at 4° C in 4% paraformaldehyde (EMS catalog # 15710) and 4% sucrose (MP Biomedicals catalog # 802536 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl sample (5 x diluted) was placed to freshly glow-discharged carbon EM grid (400 mesh copper, Electron Microscopy Sciences). After incubating for 2-minute ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then rinsed 3 times on ice with ice-cold PBS and immediately fixed in 4% paraformaldehyde (EMS, 15710-S) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% sodium azide (1hr rinse, 3 rinses) and was mounted on glass slides in anti-fade media (Citifluor, Electron Microscopy Sciences) and coverslipped ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: Hippocampi from 6-month-old PS19 mouse brains with and without 3-month glutamine administration were cut into small specimens (one dimension < 1 mm) and fixed in Trump’s fixative (Electron Microscopy Sciences) for 2 hr at RT ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Microbiology 2023Quote: ... 3 µl of bacterial suspension were placed on a freshly glow-discharged carbon covered electron microscopic support grid (EMS, Hatfield, PA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed 3 × 5 minutes in PBS and mounted to slides using FluoroGel with DABCO (Electron Microscopy Sciences, #17985) and 1.5 thickness coverglass (Fisherbrand ...
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bioRxiv - Biochemistry 2023Quote: ... Purified proteins were diluted to 0.005-0.01 mg/mL and 3.5 µL was applied onto copper grids with a thin layer of continuous carbon (approximately 3-4 nm, Electron Microscopy Sciences) that had been plasma-cleaned (Gatan Solarus ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of dAK (1 mg/ml) was applied to QUANTIFOIL Cu R300 1.2/1.3 (Electron Microscopy Sciences, catalog no. Q3100CR1.3) that had been glow discharged using a PELCO easiGlow device (Ted Pella Inc. ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2024Quote: 3 µl of sheath preparation sample was incubated for 1 min on glow-discharged carbon-coated formvar grids (Electron Microscopy Sciences). To stain the sample ...
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bioRxiv - Microbiology 2024Quote: Clinical samples and biofilm samples fixed using the SEM fixation buffer were subsequently subjected to a series of washing steps (3 repetitions for 5 mins each) with 0.1M Cacodylate buffer pH 7.4(Electron Microscopy Sciences, USA) and distilled water (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 3 µL sample was applied to glow discharged Quantifoil® R1.2/1.3 Cu 300 mesh grid (Electron Microscopy Sciences, PA, USA). The samples were then plunged into liquid ethane using a Vitrobot™ Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Copper grids were post stained with a 1.2% UA solution and a 3% lead citrate solution (Electron Microscopy Sciences Cat#22410) for 4 min each ...
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bioRxiv - Cell Biology 2020Quote: Cells were cultured on cell tight C-2 cell disks (Sumitomo Bakelite) and fixed for 2 h in 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples were immersion-fixed for at least one overnight at 4°C in 2.5% glutaraldehyde + 4% paraformaldehyde (Electron Microscopy Science) in a 1mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Cell Biology 2021Quote: ... Post-fixation was performed for 1 hour at 4 °C using 1 % osmium tetroxide and 0.8 % potassium ferracyanide (Electron Microscopy Sciences) in 0.1 mol/l cacodylate buffer ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 3.2 μL of RNCs were incubated on plasma-cleaned 300-mesh holey carbon grids (C-flat R2/2, Electron Microscopy Science) for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μl of sample was loaded on freshly glow discharged 1.2-1.3 300 mesh C-flat™ grids without further support (Electron Microscopy Sciences). The Vitrobot chamber was set to 4 °C and 95% humidity and grids were prepared with a range of blotting time and force ...
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bioRxiv - Cancer Biology 2022Quote: ... Target retrieval was performed in citrate buffer at pH 6.0 for 15 minutes at 110°C under pressure and sections were transferred to Sequenza slide staining racks (Electron Microscopy Sciences). Sections were treated with Bloxall (Vector labs ...
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bioRxiv - Cell Biology 2020Quote: ... yeast cells with fixed for one hour at 30°C by addition of 4% (v/v) para-formaldehyde (Electron Microscopy Science) to the culture media ...
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bioRxiv - Immunology 2021Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Immunology 2020Quote: ... incubated with 15% (w/v) sucrose for 12 hours at 4°C and frozen in Tissue-Tek® (EMS, Hatfield, PA) embedding medium ...
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bioRxiv - Cancer Biology 2022Quote: ... at 37 °C for 20 minutes prior to fixation in periodate–lysine–paraformaldehyde (PLP) buffer (1% paraformaldehyde (PFA; Electron Microscopy Science), 0.01M sodium periodate ...
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bioRxiv - Microbiology 2022Quote: Bacteria were grown in Luria Broth liquid culture overnight at 37°C and immobilized on a Formvar grid (Electron Microscopy Sciences) for 1 min 30 sec and the samples were negatively stained with 0.5% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... pH 7.4 buffer) was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Microbiology 2022Quote: ... From this stock a 3.5 µl drop was applied to a (45 s) glow-discharged R1.2/1.3 C-flat grid (Electron Microscopy Science, Hatfield, USA), and plunge-frozen in liquid ethane (Vitrobot Mark IV ...
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bioRxiv - Developmental Biology 2023Quote: ... as well as P14 Hmgb1flx/flx and Hmgb1ΔAstro animals was extracted and fixed overnight at 4°C in 4% RNase-free PFA (Electron Microscopy Sciences) in sterile PBS ...
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bioRxiv - Biochemistry 2023Quote: ... was applied four times to glow-discharged Quantifoil R3.5/1 grids coated with a layer of 2 nm ultrathin carbon (Au 400 2nm C, Electron Microscopy Sciences) with 45 seconds incubation followed by a manual blot between each application ...
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bioRxiv - Plant Biology 2023Quote: ... The specimens were finally polymerised at 70°C for 72 h in flat embedding silicon molds (#70905-01, EMS, Hatfield, USA).
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with treatments for 20 min at 37°C and immediately fixed by addition of paraformaldehyde (Electron Microscopy Sciences) to 3.7% ...
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bioRxiv - Microbiology 2023Quote: ... and the transwell insert was washed with Dulbecco’s phosphate-buffered saline (DPBS) and fixed overnight at 4°C with cold 4% paraformaldehyde (PFA; Electron Microscopy Sciences) diluted in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... The cross was raised at 25°C and the adult progeny was fixed overnight in 4% paraformaldehyde (Electron Microscopy Sciences, 15710) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were washed with ddH2O 5 x 5 minutes at 4°C on ice and were placed in aqueous 2% uranyl acetate (EMS, #22400) overnight at 4°C ...