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Citations for Electron Microscopy Sciences :
151 - 200 of 458 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Grids (1.2-µm/1.3-µm 400 mesh C-flat grids, Electron Microscopy Sciences #CF413-50) were glow discharged (PELCO easiGlow ...
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bioRxiv - Cell Biology 2021Quote: ... and then fixed for 20 minutes in 3% (v/v) paraformaldehyde (PFA, 15710 Electron Microscopy Sciences) in CB (protocol follows (Li et al. ...
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bioRxiv - Biophysics 2020Quote: ... and 3 μL were applied to Quantifoil copper grids covered with 300-mesh holey carbon (EMS), which were previously plasma-cleaned for 12 s with an argon-oxygen mixture (25% oxygen ...
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bioRxiv - Bioengineering 2022Quote: ... Both channels (n=3 per donor) were fixed using 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences). The PFA solution was pipetted into both channels followed by a 15-minute incubation at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples (3 µL) were dried on carbon-coated 400 mesh copper grids (Electron Microscopy Sciences, USA) overnight at room temperature in a desiccator ...
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bioRxiv - Microbiology 2020Quote: Samples (3 μL) were onto the carbon side of carbon-coated copper grids (Electron Microscopy Sciences) for 90 s ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were then harvested and 3×106 cells were fixed in 4% paraformaldehyde (EMS, 15710) at RT for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... 3 mL of LBv2 media without substance or test conditions (EMS [1:1,000](Sigma-Aldrich, #M0880) or MMS [1:10,000](Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl were applied on carbon-coated 200-mesh Cu grids (Electron Microscopy Sciences, Hatfield, PA). After 60 s of incubation ...
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bioRxiv - Cell Biology 2023Quote: ... sections were passed through 3 separate blocks of optimal cutting temperature (OCT) compound (Electron Microscopy Sciences) to wash off the sucrose solution ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 3 times with CyTOF buffer and fixed in 1.6% PFA (Electron Microscopy Sciences) at 1×106 cells/ml for 15 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were manually unroofed by spraying 1 mL of 3% paraformaldehyde (Electron Microscopy Sciences #15710) and 1% glutaraldehyde (Electron Microscopy Sciences #16216 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a 3 µl mixture was spotted on glow-discharged grids (Electron Microscopy Sciences, CF400-Cu), then stained with 2% uranyl acetate ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3 times with PBS and chemically fixed with 4% PFA (Electron Microscopy Science) in PBS for 30 min at RT.
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bioRxiv - Cancer Biology 2024Quote: ... They were fixed at room temperature for 20 min with 3% paraformaldehyde (PFA; Electron Microscopy Sciences) and stained with Crystal Violet ...
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bioRxiv - Microbiology 2020Quote: ... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.2 at 4° C and post-fixed with 1% osmium tetroxide (# 19150, Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer (#12300 ...
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bioRxiv - Plant Biology 2021Quote: ... then for G/C to A/T transitions that were likely to be caused by EMS, and thirdly for non-synonymous amino-acid changes.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were fixed for 20 min at 4°C in 4% paraformaldehyde solution (Electron Microscopy Sciences) diluted in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 30 mL of cold (4°C) 4% paraformaldehyde (PFA; Cat#19210, Electron Microscopy Sciences) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Cell Biology 2023Quote: ... sponges were fixed for 2 hours at 4 °C with 2.5% glutaraldehyde (Electron Microscopy Science, 16020), post-fixed for 1 hour at room temperature with 1% OsO4 (Electron Microscopy Science ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 30 ml of cold (4°C) 4% paraformaldehyde (PFA; Cat# 19210, Electron Microscopy Sciences) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM DTT) and fixed by the addition of 3 ml PBS 1.33% paraformaldehyde (Electron Microscopy Sciences) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2021Quote: ... 2% and 3% OsO4 (prepared from 2 mL 4% osmium tetroxide solution, Electron Microscopy Sciences, Hatfield, PA)
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bioRxiv - Immunology 2021Quote: ... Sections were washed in PBS 3 times and then mounted with Fluoromount-G (Electron Microscopy Sciences, 1798425). Controls included ...
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bioRxiv - Neuroscience 2022Quote: ... The assay was ended after 3 hrs by fixation with final 4% paraformaldehyde (Electron Microscopy Sciences, #15713S).
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 30 min with 3% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized for 10 min with 0.1 % Triton X-100 and blocked for 30 min with 10% goat serum before incubation with specific primary antibodies for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The EV sample (3 µl) was applied to Lacey carbon grids (LC-200 Cu, Electron Microscopy Sciences) covered with a perforated carbon film and discharged for 40 s with a 5 mA current ...
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bioRxiv - Cell Biology 2023Quote: For autophagic flux assays: mRFP-GFP-LC3B HeLa cells were fixed in 3% paraformaldehyde (Electron Microscopy Sciences) in PBS-CM (1X PBS with 0.1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were rinsed 3 times with PBS++ and fixed with 2% paraformaldehyde (PFA; 15710, Electron Microscopy Sciences) in PBS++ for 15 min at R.T ...
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bioRxiv - Immunology 2024Quote: ... the 3 μl mixture was applied to a UltrAuFoil R1.2/1.3 300 mesh grid (Electron Microscopy Sciences) that had been glow-discharged with a PELCO easiGlow glow discharge cleaning system for 1 min ...
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bioRxiv - Neuroscience 2024Quote: ... and counterstained with UranyLess for 5 minutes followed by 3% lead citrate (22409, 22410, Electron Microscopy Sciences) for 5 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... Samples in resin were polymerized at 65°C for 36 hours in BEEM capsules (Electron Microscopy Sciences). Silver (~80 nm thick ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed for 10 min in either -20°C methanol or 4% paraformaldehyde (Electron Microscopy Sciences). Cells were blocked and permeabilized in a solution containing 1:1 Odyssey blocking buffer (LiCor)/PBS (Invitrogen ...
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A gut-secreted peptide controls arousability through modulation of dopaminergic neurons in the brainbioRxiv - Neuroscience 2020Quote: ... Guts were fixed overnight at 4 °C in PBS with 4% paraformaldehyde (PFA, Electron Microscopy Sciences #15710). Dissected brains and ventral nervous systems were fixed for 30 minutes in 4% PFA at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% NP-40) to glow-discharged C-Flat holey carbon grids (CF-2/1-3C-T, EMS). The samples were plunge-frozen using a Vitrobot (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and fixed at 4°C for 10 min in 4% paraformaldehyde (Electron Microscopy Sciences). The fixed cells were washed and resuspended in PBS containing 5% FBS ...
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bioRxiv - Zoology 2021Quote: ... sponge tissues were fixed for 2 hours at 4 °C with 2.5% glutaraldehyde (Electron Microscopy Science, 16020) and post-fixed for 1 hour at room temperature (RT ...
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bioRxiv - Neuroscience 2022Quote: ... A thin layer of clear dental cement (C&B super-bond) and nail polish (Electron Microscopy Sciences) were applied for strengthening and to reduce light glare during imaging ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of freshly purified CORVET samples at a protein concentration of approximately 0.8 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and immediately plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and then post-fixed for 1 hr at 4°C in 1% osmium tetroxide (Electron Microscopy Sciences) in 0.1 M Sodium Cacodylate ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then fixed overnight at 4°C in 1% paraformaldehyde (20% PFA, Electron Microscopy Sciences, #15713). Immunohistochemistry was performed as described previously (McFarland et al. ...
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bioRxiv - Synthetic Biology 2024Quote: Whole kidneys were fixed at 4°C for 4 hours in 4%PFA (Electron Microscopy Sciences, 15710), thoroughly washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Frozen mouse brains were placed on a stainless steel adult mouse brain slicer matrix (EMS #69090-C) kept on dry ice ...
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bioRxiv - Biochemistry 2024Quote: ... The assembled complex (4 µL) was applied to C-flat R2/1 holey carbon copper grids (EMS) after glow discharging the grids at 11 mA for 30 sec (Pelco) ...
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bioRxiv - Immunology 2024Quote: ... we washed and fixed cells for 15 minutes at 37°C in 4% paraformaldehyde (Electron Microscopy Sciences) diluted in DMEM ...
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bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3× with PBS and fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, PA, USA) in PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were fixed with 3% (v/v) formaldehyde in PBS (methanol-free, Electron Microscopy Science, Hatfield, PA, USA) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 3 times in 50% resin (24g Embed 812, 9g DDSA, 15g NMA, 1.2g BDMA; Electron Microscopy Sciences 14121) dissolved in propylene oxide ...