Labshake search
Citations for Thermo Fisher :
301 - 350 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... Gene-specific primers to SARS-CoV-2 (Wuhan v1, NSP14) and SYBR green master mix (Applied Biosystems) were used to amplify viral RNA ...
-
bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 RBD (BEI Resources NR-52309) was biotinylated with Sulfo-NHS-LC Biotin (Thermo Fisher), then incubated with 1μm fluorescent neutravidin beads (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... SARS-CoV-2 quantification was performed employing the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Inc.) with indicated primers (Table 1) ...
-
bioRxiv - Immunology 2021Quote: ... The proteins were transferred to nitrocellulose membrane and probed with polyclonal rabbit serum against SARS-CoV S1 domain (Thermofisher, Cat No. PA5-81798 and PA5-81795), monoclonal antibodies I1 (8G5F11 ...
-
bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
-
bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 variants were identified by the targeted sequencing approach with the Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
-
bioRxiv - Cell Biology 2021Quote: ... The coding sequence of human PLIN2 with an N-terminal EGFP tag was provided by John McLauchlan (Targett-Adams et al., 2003) and re-cloned into pCR8 (ThermoFisher Scientific). The human PLIN3 (TIP47 ...
-
bioRxiv - Cell Biology 2021Quote: ... The pcDNA5/FRT-PRPF4 plasmid was constructed by cloning the PRPF4 gene fused to an N-terminal FLAG/HA tag into the pcDNA5/FRT plasmid (Thermo Fisher).
-
bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
-
bioRxiv - Cell Biology 2023Quote: ... All the IHO1 truncations were expressed with the 3C HRV cleavable N-terminal MBP tag in suspension culture of High Five™ Cells (Invitrogen) in Sf-900™ III SFM medium ...
-
bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
-
bioRxiv - Cell Biology 2023Quote: From Expi293 cells: Constructs in pCMV10 vector with N terminal 3xFLAG tag were transfected into Expi293F cells (A14527; Thermo Fisher Scientific) with PEI (Polyscience ...
-
bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 were mixed in a ratio of 0.7:0.7:0.4 with Opti-MEM® media (Gibco) to give 275 μL ...
-
bioRxiv - Microbiology 2022Quote: ... and spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT were co-transfected into HEK293T cells (ATCC CRL_3216) with lipofectamine 2000 (ThermoFisher Scientific). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
-
bioRxiv - Cell Biology 2021Quote: ... SARS-CoV-2 titration was obtained using a TaqMan 2019nCoV assay kit v1 RealTime-PCR (Thermo Fisher, Italy).
-
bioRxiv - Biochemistry 2021Quote: The gene encoding the PLpro (also known as nsp3) protein from SARS-CoV-2 (YP_009725299.1) was also synthesized commercially (Invitrogen) and cloned into the pSUMO1 vector with N-terminal 8xHis conjugated with yeast SUMO ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: SARS-CoV-2 total viral RNA was extracted from Vero cells (ATCC CCL-81) cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Microbiology 2020Quote: Total RNA was extracted from Vero CCL81 cells infected with VSV-SARS-CoV-2-SΔ21 using Trizol (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
-
bioRxiv - Immunology 2021Quote: ... and spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
-
bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
-
bioRxiv - Microbiology 2021Quote: ... and spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using Wuhan/WIV04/2019strain (GISAID accession ID ...
-
bioRxiv - Immunology 2021Quote: ... and Spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 coding sequences were inserted using the Gateway LR Clonase II Enzyme Mix kit (Invitrogen, CA), yielding plasmids pLCPD-S ...
-
bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... TH-tag 1:500 (chicken, Ab76442 or sheep, Invitrogen PA1-4679), diluted into blocking solution ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were probed with rabbit-anti-SARS spike (Invitrogen, PA1-411-1165), mouse-anti-SARS-CoV-2 spike (GeneTex 1A9) ...
-
bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2021Quote: ... was cloned into a modified pEZT-BM vector with an N-terminal Flag tag (Morales-Perez et al., 2016) and heterologously expressed in HEK293F cells using the BacMam system (Thermo Fisher Scientific). Bacmids were synthesized using E ...
-
bioRxiv - Biophysics 2022Quote: ... and apoA1 were purified using established protocols for MSP44 with the following modifications: A synthetic construct of apoA1 bearing a 3C protease cleavable N-terminal deca-histidine tag (GeneArt/Thermo Fisher Scientific) was cloned into a pET28a vector (Addgene ...
-
bioRxiv - Biophysics 2022Quote: ... a pET21a vector including hTS with an N-terminal 6x histidine tag was transformed into BL21 (DE3) cells lacking the rne131 gene (Invitrogen, Carlsbad, CA). After an adaptation period (Bonin & Lee ...
-
bioRxiv - Cell Biology 2022Quote: ... transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC) destination vectors expressing the N terminal tag fused target proteins under Actin5C promoter (Invitrogen, Carlsbad, CA). In addition ...
-
bioRxiv - Molecular Biology 2021Quote: ... Genes were cloned into modified pRSF1-Duet expression vector with a TEV-cleavable N-terminal 14-histidine tag (39) using New England Biolabs and Anza (Thermo Fisher Scientific) enzymes ...
-
bioRxiv - Biochemistry 2023Quote: ... Full-length genes of human RNGTT and CMTR1 were cloned into pMlink vector containing a 4×ProteinA tag at the N-terminal and were transfected to Expi293F cells (Thermo Fisher Scientific) when cell density is 2.5× 106 /ml ...
-
bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
-
bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
-
bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
-
bioRxiv - Biochemistry 2020Quote: ... different domains or subunits of the Spike protein and the extracellular domain of ACE2 were produced in Expi293F cells (Thermo Fisher Scientific). Expi293F cells were cultured at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2021Quote: RNA extraction of SARS-CoV-2 infected A549-ACE2 cells was performed by TRIzol (Thermo Fisher Scientific, 15596-026) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
-
bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S protein Omicron genes were synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific). Pseudotyped retroviral particles were produced in HEK293T cells as described previously (32) ...
-
bioRxiv - Microbiology 2021Quote: ... and 0.06 μg/ml or vehicle and 2 h later infected with SARS-CoV-2 (multiplicity of infection (MOI) = 0.01 and 0.1) in serum-free DMEM (Thermo Fisher) for 1 h at 37°C ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...