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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and transfected with 24 µg of plasmid encoding either the wildtype or E406W SARS-CoV-2 spike protein using Lipofectamine 2000 (Life Technologies). Four hours at transfection ...
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bioRxiv - Microbiology 2021Quote: A codon optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2022Quote: A codon-optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2021Quote: ... The positive control was addition of 10 μg/mL of SARS-CoV-2 spike protein antibody (pAb, T01KHuRb) (ThermoFisher, MA, USA) at the time of infection.
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2021Quote: Point mutations to generate the mutants were introduced by site-directed mutagenesis of phCMV-SARS-CoV-2-Spike using Q5 polymerase (Thermo Scientific) and validated by sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Immunology 2022Quote: ... Sections were then incubated with cocktails of primary antibodies: rabbit anti-SARS-CoV-2 Spike Protein (1:100, Invitrogen, #MA5-36087) + rat anti-ERα H222 (1:100 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 5% FBS and 0.5% Triton X-100) for 30 minutes and washed before incubation with an anti-SARS-CoV-2 spike protein antibody (Life Technologies). Nuclei and F-actin were stained using DAPI and Phalloidin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 virus was labelled using first an anti-SARS-CoV-2 antibody (Spike protein, Thermo Fischer) and then a secondary anti-rabbit Alexa 647 (Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... and the SARS-CoV-2 spike gene was reverse-transcribed and amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Immunology 2020Quote: ... The cells were blocked with 5 % skim milk-PBS for 1 hour at RT and incubated with an antibody against SARS-CoV-2 spike (BLSN-005P, Beta Lifescience) or control IgG (Thermo Fisher) at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Genomics 2023Quote: ... The trimeric RhGB07 spike protein was expressed as previously reported as for the SARS-CoV-2 spike transiently expressed in suspension-adapted ExpiCHO cells (Thermo Fisher) in ProCHO5 medium (Lonza ...
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcribed RNA of SARS-CoV-2 N-gene was synthesized with MEGAscript T7 (Thermo Fisher Scientific) using PCR product generated from SARS-CoV-2 WA1 cDNA and primers N0.5_F and N3-5_R (Eurofins ...
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bioRxiv - Microbiology 2022Quote: ... and stained using SARS/SARS-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody (Invitrogen MA5-29981) as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies of anti-SARS/SARS-CoV-2 Nucleocapsid Monoclonal Antibody (Invitrogen, MA1-7404) is diluted in section staining buffer at 1:400 and sections are stained at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The following custom TaqMan gene expression assay IDs were used to assess the expression of RSV N and SARS-CoV-2 N genes: ARU66XH and APNKYWD (Applied Biosystems, CA, USA). A no template control was included in each run ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal antibody generated against the C-terminal domain of N-cadherin (Invitrogen; 1:500); anti-CRB1 (a kind gift of Dr ...
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bioRxiv - Microbiology 2022Quote: ... Cleavage of the spike protein was detected using anti-SARS-CoV Spike Protein (SDelta3) monoclonal antibody (7G12, Thermo Fisher Scientific, CA, USA)) raised against immunogen aa168-461 of the S1 domain.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 S2 (Thermo Fisher # PA5-112048). The inability of the rescued virus to infect BHK-21 cells in the absence of hACE2 was assessed by fluorescence microscopy and flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 Nucleocapsid (Thermo Fisher; MA5-29982). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were purchased from Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 S1 protein primary antibody (Thermo Fisher Scientific) at 1µg/ml were added for 1 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... HIV-based lentiviral pseudotyped particles harboring the SARS-CoV-2 Spike protein variants were produced using the ViraPower Lentiviral Expression system (Thermo Fisher Scientific) according to manufacturer’s protocol with minor changes ...
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bioRxiv - Immunology 2023Quote: ... The cells were then transfected with ancestral or variant SARS-CoV-2 spike protein encoding plasmid using Lipofectamine 3000 Transfection Reagent (L3000001; Thermo Fisher Scientific). After 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid coding for SARS-CoV-2 RBD or ACE2-Fc and were purified on Ni-NTA resin (Invitrogen) or Protein A resin (Cytiva) ...
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bioRxiv - Microbiology 2021Quote: ... Taqman gene expression assays for N2 and N3 regions of the SARS-CoV-2 N gene were made (ThermoFisher) following CDC specifications (Division of Viral Diseases ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Immunology 2021Quote: ... Molecular detection of SARS-CoV-2 was performed with TaqMan™ Gene Expression Assay (Cat. N. 4331182, ThermoFisher®) with specific primers/probes previously described40 ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... containing an N-terminal HA epitope tag with an LR reaction (Life Technologies) per manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: 2 × 20 μg of each of the three different SARS-CoV-2 spike proteins were mixed with 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and up to 10 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000, Thermo Fisher Scientific PA5-112048), and an anti-p24 monoclonal antibody (1:1,000) ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
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bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...