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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Microbiology 2022Quote: ... 1:5,000 dilution) (rabbit anti-Calreticulin, Cell Signaling, Cat #: 12238, 1:2,000 dilution) (mouse anti-Spike S2 or SARS-CoV Spike, Thermo Fisher, Cat #: MA5-35946, 1:2,000 dilution) (rabbit anti-anti-Spike S2 ...
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bioRxiv - Microbiology 2021Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS-CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described [41] using the following primers and probes ...
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bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
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bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
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bioRxiv - Cell Biology 2020Quote: ... the N-terminal His6-tag was removed by the addition of His6-rTEV protease (Life Technologies) and dialyzed in H Buffer for 36 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and inserted into a pET-SUMO expression vector containing an N-terminal hexahistidine-SUMO tag (Invitrogen). PARP1 truncations (PARP1-ZnF ...
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bioRxiv - Molecular Biology 2022Quote: ... fused to a C-terminal trimeric GCN4 (tGNC4) zipper tag via a 5XGGS linker region was secreted from suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg of plasmid encoding SARS-CoV2 spike (D614) with C-terminal 18 amino acid deletion using Lipofectamine LTX Plus reagent (Invitrogen, USA) as per manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... and SARS Spike protein (Thermo Fisher, 1:250), and with Phalloïdin-647 (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 viruses were propagated in VeroE6 cells in Opti-MEM I (Invitrogen) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S RBD-serum mixtures were loaded onto MaxiSorp 96F plates (Nunc) coated with 200 ng/well ACE2 [produced in-house as described in Bosnjak et al.(25)] and blocked with 2% bovine serum albumin/2% mouse serum (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S protein was conjugated to streptavidin-AF488 (Life Technologies #S11223), the HCoV-HKU1 S protein to streptavidin-BV421 (BD Pharmingen #563259 ...
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bioRxiv - Immunology 2022Quote: The SARS-CoV-2 VFLIP ectodomain (81) was produced in Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The pBAC-SARS-CoV-2-mCherry-2A was digested with BstBI (FastDigest, Thermo Fisher) and BamHI (FastDigest ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 S2 variants were transiently transfected in ExpiCHO-S cells (Thermo Fisher). CHO cells were maintained and transfected according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Cell Biology 2023Quote: A cDNA encoding p38α was subcloned into pcDNA6 with an N-terminal myc tag (Invitrogen, Carlsbad, CA), and a kinase-dead (D168A ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from the SARS-CoV-2 infected Caco-2 cells using Trizol (Thermo Fisher). The extracted RNA was then reverse transcribed with the reverse primers specific to Nsp12 ...
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bioRxiv - Biophysics 2022Quote: The SARS-CoV-2 Omicron RBD and the N-terminal peptidase domain of ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously stated ...
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bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
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bioRxiv - Biophysics 2021Quote: ... norvegicus with a HA-tag added to the C-terminal end representing the N-terminal domain (Met 1 to Val 469) of sAC (57) was cloned into pcDNA5/FRT (Invitrogen).
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bioRxiv - Biophysics 2020Quote: The 2019-nCoV receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen). The 2019-nCoV RBD (residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2022Quote: ... pylori NAP proteins (SARS-CoV-2S6p3 and SARS-CoV-2S6p312, respectively) were produced upon transient expression in Expi293F cells (ThermoFisher). Clarified supernatants were purified by affinity chromatography using an anti-HA affinity matrix (Cat# 11 815 016 001 ...
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bioRxiv - Immunology 2020Quote: ... spike protein was expressed by transiently transfecting plasmid encoding the HexaPro spike variant2 containing substitutions S383C and D985C3 with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Microbiology 2022Quote: ... human Anti-SARS COV-2 Nucleocapsid monoclonal (E16C) (Thermo Fisher, MA1-7403, 0.1 mg/mL), was incubated as a 1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... to 1 µg/ml and mouse SARS-CoV-2 Nucleoprotein monoclonal antibody (MA1-7404, Thermofisher) to 10 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Microbiology 2020Quote: ... the purified SARS-CoV-2 RBD protein was coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at around 5μg/mL in PBS overnight ...
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bioRxiv - Microbiology 2020Quote: ... codon-optimized (for human cells) SARS-CoV-2 DNA (GeneArt Gene Synthesis, Thermo Fisher Scientific) based on the publicly available protein sequence in the National Center for Biotechnology Information database (NCBI Reference Sequence ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Validation and SARS-CoV-2 genome detection was performed with TaqMan detection systems (Applied Biosystems) based qPCR using SARS-CoV-2 specific primers and probes with the following sequences ...
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bioRxiv - Microbiology 2021Quote: ... the purified SARS-CoV-2 RBD proteins were coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at 5μg/mL in PBS overnight ...
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bioRxiv - Immunology 2021Quote: ... RBD binding inhibition was measured using a SARS-CoV-2 Neutralizing Ab ELISA Kit (Invitrogen). For anti-Spike IgG experiments ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 RBD 6xHisTag constructs were expressed by transient transfection of Expi293F cells (Gibco) and purified using HisTrap FF and HisTrap HP columns (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 pseudoparticles were produced by co-transfecting HEK293T cells using Lipofectamine 2000 (Invitrogen) with plasmids encoding HIV-1 gag/pol ...
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bioRxiv - Pathology 2022Quote: ... and either pMD2.G or pTwist-EF1alpha-SARS-CoV-2-S-2xStrep using LTX (Invitrogen) transfection reagents according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 infected cells were washed with PBS and lysed with TRIzol reagent (Invitrogen). Total RNA was extracted using RNeasy mini kit (QIAGEN ...
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bioRxiv - Immunology 2024Quote: ... indicated SARS-CoV-2 Z-RNAs) was incubated with Lipofectamine 2000 transfection reagent (Invitrogen, 11668019), for 15 mins at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of N501Y-SARS-CoV-2 BAC were transfected into Vero E6 (ATCC) with Lipofectamine 3000 (Invitrogen) in a 6-well plate according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... with an N-terminal 6xHis tag followed by SUMO fusion protein using Gibson assembly protocol (Thermo Fisher Scientific). Protein was expressed in E ...
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bioRxiv - Microbiology 2021Quote: ... Binding studies in HEK 293T cells were performed using rVSV-SARS-CoV-2 Spike virions (rVSV/Spike) and binding was performed at room temperature in IMMULON 2HB flat bottom plates (Thermo Scientific, Waltham, MA).
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Biochemistry 2024Quote: ... with a replacement in the N-terminal domain loop 8 (139CQKRDGPH146 to 139AEAG142, designated as rA3GR8) was constructed in the pET-sumo vector (Thermo Fisher Scientific). This construct generated a SUMO fusion with an N-terminal 6xHis tag and a PreScission protease cleavage site ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the short transcriptional variant of human DA D2 receptor with an N-terminal FLAG tag (SF-D2R-S) was inserted into mammalian expression vector pcDNA 3.1(+) (Invitrogen)62 ...
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bioRxiv - Developmental Biology 2019Quote: ... GenBank accession number BC057894.1) with an N-terminal hemagglutinin (HA) tag were reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs ...