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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and a C-terminal hexa-histidine tag (GeneArt Gene Synthesis, Thermo Fisher Scientific). The HexaPro expression plasmid was a gift from Jason McLellan (Addgene plasmid #154754 ...
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bioRxiv - Cancer Biology 2021Quote: ... An N-terminal HMMR fragment was amplified using AmpliTaq (Thermofisher, N8080153) or Q5 High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Immunology 2022Quote: ... AD-2 domain site 1 (Life Technologies Corporation), Domain I ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was inserted into the pFastBac-Dual vector (Invitrogen) and was expressed using Bac-to-Bac baculovirus system (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was expressed in FreeStyle 293-F cells (Invitrogen). The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (SouthernBiotech ...
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bioRxiv - Biophysics 2021Quote: ... were coated either with 200 ng of SARS-CoV-2 SΔTM proteins or bovine serum albumin (BSA, Thermo Scientific™, Waltham, MA, USA) through incubation overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... 9 x 105 cells of each indicated SARS-CoV-2-non-infectable cell type were stained with CellTrace™ Violet (Thermo Fisher # C34557), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Sequence corresponding to the 5’-300 bases of SARS-CoV-2 was PCR amplified from pUC57 vector and cloned into the pJet1.2 vector (Thermo Fisher Scientific, Massachusetts, USA) under the control of T7 promoter ...
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bioRxiv - Microbiology 2020Quote: ... total cellular RNA containing SARS-CoV-2 RNA was extracted from the Vero E6 cells used for viral stock production using TRIzol™ Reagent (Invitrogen™, ThemoFisher) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: Libraries for whole genome sequencing were generated using the Ion AmpliSeq Kit for Chef DL8 and Ion AmpliSeq SARS-CoV-2 Research Panel (Thermo Scientific, Waltham, MA). Libraries were sequenced using an Ion 520 chip on the Ion S5 system using the Ion 510™ & Ion 520™ & Ion 530™ Kit ...
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bioRxiv - Microbiology 2021Quote: Libraries for the whole genome sequencing were generated using the Ion AmpliSeq Kit for Chef DL8 and Ion AmpliSeq SARS-CoV-2 Research Panel (Thermo Scientific, Waltham, MA). Libraries were sequenced using an Ion 520 chip on the Ion S5 system using the Ion 510™ & Ion 520™ & Ion 530™ Kit ...
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bioRxiv - Microbiology 2021Quote: ... and assembled into full length SARS-CoV-2 cDNA clones in the YAC vector pYESL1 using a GeneArt™ High-Order Genetic Assembly System (A13285, Invitrogen™, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... rVSV-ΔG/SARS-2-S*-luciferase pseudovirus was generated by transfecting BHK21/WI-2 cells with pcDNA expressing codon-optimized full-length SARS-CoV-2 S protein (GenBank accession number: MN908947) using Lipofectamine™ 2000 transfection reagent (Invitrogen, Carlsbad, CA). Transfected cells were then infected with rVSV-ΔG/G*-luciferase 24 h later ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of test peptide (at a final concentration of 1 μg/ml) PepMixTM SARS-CoV-2 S (JPT, PM-WCPV-S-1) or murine anti-CD3 antibody (positive control) (Invitrogen, 16-0031-82) or CTL-TestTM medium (negative control ...
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bioRxiv - Immunology 2020Quote: Seventy-six consecutive patients with SARS-CoV-2 infection confirmed through reverse transcriptase-polymerase chain reaction (RT-PCR, Life Technologies, Carlsbad, CA, USA) from March 16 through March 27 ...
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bioRxiv - Immunology 2022Quote: ... a TMPRSS2 plasmid and S plasmids from SARS-CoV-2 variants into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) 39,40 ...
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bioRxiv - Microbiology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd- SARS-CoV-2-BA.5-S ...
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bioRxiv - Neuroscience 2023Quote: ... To authenticate SARS-CoV-2 isolates used in the study viral RNA was extracted from stocks using TRIzol LS reagent (Thermo Fisher Scientific, USA) and cDNA was prepared with Protoscript II first-strand cDNA synthesis kit as per manufacturer’s protocol (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from both SARS-CoV-2 infected (48h post-infection) and non-infected (mock) Vero cells using TRIzol reagent (Thermo Fisher Scientific, 15596026) and Direct-zol RNA Miniprep (ZYMO research ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd-SARS-CoV-2-BA.5-S ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting cDNA was used to quantify the SARS-CoV-2 RNA levels by real-time quantitative PCR using Power SYBR green PCR master mix (Applied Biosystems, Waltham, MA). Average values from duplicates of each sample were used to calculate the viral RNA level relative to the GAPDH gene and presented as 2−ΔCT ...
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bioRxiv - Immunology 2023Quote: Virus samples were serially diluted (10-fold starting at 1:10 initial dilution) in serum-free DMEM (recRSV-mKate and MA-SARS-CoV-2) and supplemented with 0.5% Trypsin (Gibco, recA/California/07/2009). Serial dilutions were added to cells seeded in 96-well plate at 1×104 cells per well ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with SmaI and ligated in frame with SmaI a N-terminal 3xFLAG-tag and a C-terminal 6xhistidine tag into a modified and SmaI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH2.
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Cancer Biology 2020Quote: ... RELA with C-terminal HA tag into Gateway donor vector (pCR8/GW/TOPO, Invitrogen). Then LR clonase II reactions were used to shuttle ORFs into pCW-DEST (lentiviral Dox-inducible expression ...
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bioRxiv - Cell Biology 2023Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... and SARS-CoV S protein were confirmed by transfection with the Turbofect transfection reagent (ThermoFisher) followed by radiolabeling and immunoprecipitation analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were incubated with rabbit anti-SARS coronavirus spike protein polyclonal antibody (Invitrogen, PA1-41513) diluted 1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... N-2 (Gibco), 1.25 mM N-Acetyl-L-cysteine (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... N-2 (Gibco), B-27 (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from SARS-CoV-2-infected Vero-E6 cells and purified with an Oligo (dT) kit (Thermo Scientific, Wilmington, DE, USA). The polyA purified RNA was fragmented and subjected to IP using an m6A-specific antibody ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 RdRp IP2-targeted RdRp Institute Pasteur RT-PCR was performed on a QuantStudio™ 5 System (Applied Biosystems, Thermo Fisher Scientific). Log10 reduction values (LRV ...
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bioRxiv - Microbiology 2023Quote: ... mock and SARS-CoV-2 infected cultures were washed with calcium/magnesium-free PBS and disassociated using TrypLE (Gibco, 5 minutes at 37° C), after which samples were centrifuged (200 x g ...
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bioRxiv - Microbiology 2022Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (Thermo Fisher), and 1X non-essential amino acids (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 1% N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco, #15630080) and 1% MEM nonessential amino acids (MEM-NEAA ...
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bioRxiv - Plant Biology 2022Quote: The full-length coding sequence of the genes were cloned into pSPYNE-35SGW (N-terminal YFP) and/or pSPYCE-35SGW (C-terminal YFP) through gateway cloning (Invitrogen™). BiFC was carried out as described (Gou et al. ...