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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Biophysics 2022Quote: ... The SARS-CoV-2 spike RBD (ThermoFisher) was immobilised on a CM5 sensorchip under the same conditions and a final response of ∼1970 RU was obtained ...
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bioRxiv - Microbiology 2022Quote: The monoclonal antibodies SARS-CoV-1/SARS-CoV-2 Spike Protein S2 (1A9) and SARS-CoV-1/SARS-CoV-2 Nucleocapsid (6H3) were purchased from ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... and probed with SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher # MA5-35946) and donkey anti-mouse secondary antibodies tagged with an infrared fluorophore (Rockland Immunochemicals) ...
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bioRxiv - Molecular Biology 2023Quote: ... SARS-CoV-2 N protein (MA1-7404, Invitrogen), TLR3 (ab62566 ...
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bioRxiv - Microbiology 2021Quote: ... antibodies targeting the SARS-CoV-2 nucleoprotein (N) (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike ORF was synthesised (GeneArt; Thermo Fisher Scientific) with a poxvirus early transcriptional stop sequence (T5NT ...
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-2 Spike plasmids using Lipofectamine 3000 (Thermo Fisher, L3000015). 48 to 72 hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... 600ng CSFLW (lentivirus backbone expressing a firefly luciferase reporter gene) and either 25ng of SARS-CoV-2 Spike or 500ng SARS-CoV in OptiMEM (Gibco) (Sup.Table.3 ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA (pVAX1-SARS-CoV-2 Spike) was transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...
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bioRxiv - Microbiology 2021Quote: ... B.1.617.2 Spikes were generated by overlapping PCR using a codon-optimized wild-type SARS-CoV-2 Spike gene (GeneArt, ThermoFisher) that was synthesized (Biobasic ...
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bioRxiv - Immunology 2021Quote: ... and anti-Spike IgG was quantified using a Human SARS-CoV-2 Spike (Trimer) IgG ELISA Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with C-terminal HRV3C-cleavable 8xHis tag) or SARS-CoV-2 RBD (residue 319-537) were transfected into Expi293F GnTI-Cells (Thermo Fisher Scientific) using PEI ...
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bioRxiv - Biochemistry 2021Quote: The expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI-(Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... the expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI- (Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Immunology 2022Quote: ... including rabbit anti-SARS-CoV-2 Spike (S1/S2) polyclonal antibody (1:2,000; Invitrogen) and mouse anti-β-actin monoclonal antibody (1:5,000 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 spike was expressed from a synthetic cDNA template (GeneArt, Life Technologies) encoding the S protein ectodomain residues 14−1208 from the Wuhan-Hu-1 strain (NCBI Reference Sequence ...
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2021Quote: ... with SARS-Cov-2 spike plasmid or a control plasmid using Lipofectamine 2000 (Life technologies) are kind gifts from Olivier Schwartz ...
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA (pVAX1 or pVAX1-SARS-CoV-2 Spike) was transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Biochemistry 2023Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Microbiology 2020Quote: ... RBD from SARS-CoV and SARS-CoV-2 were biotinylated (Thermo Fisher) and immobilized on Streptavidin (SA ...
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bioRxiv - Microbiology 2022Quote: ... or N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher) as previously described [18] ...
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bioRxiv - Immunology 2020Quote: ... and N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher), i.e. ...
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bioRxiv - Neuroscience 2020Quote: ... Bound SARS-CoV-2 Spike was detected by antiLJHis probe HRP (Cat#15165; Thermo Fisher Scientific). Some wells received no antibody (no antibody control) ...
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bioRxiv - Microbiology 2022Quote: All SARS-CoV-2 Spike constructs for pseudotype generation were expressed from plasmid pCDNA3.1 (ThermoFisher #V79020). Codon optimized SARS-CoV-2 Wuhan Spike carrying the D614G amino acid change (Sino Biological #VG40589-UT(D614G) ...
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bioRxiv - Microbiology 2020Quote: ... anti-NeuN and anti-SARS-CoV-2 spike protein antibodies (Thermo Fisher Scientific, Norcross, GA, USA) overnight at 4°C followed by incubation with Alexa Fluor 546- or Alexa Fluor 488-conjugated secondary antibody for 1 hr at room temperature [31,34] ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2023Quote: ... quantification of ADCP was performed by covalently binding SARS-CoV-2 NTD and NTD_ADEm3 to NeutrAvidin fluorescent beads (ThermoFisher, Waltham, MA). Immune complexes were formed by incubation with serially diluted (2 fold ...
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bioRxiv - Microbiology 2024Quote: ... To visualize spike in Western Blots we used anti SARS-CoV-2 Spike S2 (1A9) mouse monoclonal antibody (ThermoFisher, MA5-35946) at 1:1000 diluted in 3%BSA in TBS-T (0.01% Tween) ...
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse SARS-CoV-2-spike (GTX, 1:100) and anti-rabbit hACE2 (Thermo Fisher,1:100) were applied overnight at 4 °C followed by incubation of appropriate secondary antibodies conjugated with fluorophores ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2021Quote: ... transfected with 5 μg of an expression plasmid coding either VSV-G (pMD.G) or SARS-CoV-2 Spike del19 (pcDNA3.1_spike_del19) using Lipofectamine 2000 (Thermo Scientific). The culture medium was replaced after 6h ...
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bioRxiv - Immunology 2022Quote: ... The trimeric spike SARS-CoV-2 and its variants were biotinylated (EZ-Link Sulfo-NHS-Biotin, ThermoFisher), desalted (Zeba Spike Desalting ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (Thermo Fisher Scientific; catalog # PA5-114451).
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Bioengineering 2022Quote: ... or SARS-CoV-1-d28 (Genbank AAP13567) or SARS-CoV-2 VOC spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...