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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Microbiology 2019Quote: ... Eight μL of Cellfectin II reagent and 3 ng of each recombinant plasmid were diluted in 100 μL Grace’s medium (Thermofisher Scientific), respectively ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3E6 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Developmental Biology 2021Quote: ... for 3 hr at 37 °C with 10 μg/mL recombinant laminin-521 (BioLamina, LN521-05) diluted in PBS+/+ (Gibco). Single-cell suspensions were then incubated for 3 hr at 37 °C in HUESM medium supplemented with 20 ng/mL bFGF and 10 μM Rock inhibitor Y27632 ...
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bioRxiv - Systems Biology 2023Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Molecular Biology 2023Quote: ... High titre (>3 × 108 viral particles per ml) recombinant baculovirus was obtained using the Bac-to-Bac expression system (Invitrogen) in Sf9 (Spodoptera frugiperda ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant RNase H (Invitrogen) was later added to the reverse transcribed samples and incubated for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Recombinant B18R (Life Technologies) was used at 1ug/ml ...
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bioRxiv - Bioengineering 2020Quote: ... Human cell lines PC-3 (ATCC® CRL-1435™) were grown in RPMI1640 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... PerCP-eFluor 710 mouse anti-human CD223 (LAG-3) (1:100; 46-2239-41, Invitrogen), and BV421 mouse anti-human CD366 (TIM-3 ...
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bioRxiv - Microbiology 2022Quote: Primary human neutrophils (3×106) were suspended in Dulbecco’s PBS without calcium or magnesium (Gibco) with 0.1% glucose (DPBSG-) ...
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bioRxiv - Immunology 2023Quote: Cells were stained for viability (Supplementary table 1 and Supplementary table 4) and blocked for CD16/32 with anti-human FC Receptor binding inhibitor (ThermoFisher Scientific, 14-9161-73) in PBS for 15 minutes at room temperature (Spn ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Sections were washed and subsequently incubated with AF647-conjugated lectin peanut agglutinin (PNA; Thermo Fisher) in the presence of sodium azide and bovine serum albumin overnight at 4°C.
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bioRxiv - Microbiology 2021Quote: UPP was visualized using the WGA lectin conjugated to Alexa fluor 594 (af-WGA, Invitrogen) and FITC (fl-DBA) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 mg/ml of Alexa 594 conjugated GSII lectin (L21416, Thermo Fisher Sci, Waltham, MA) in PBS was perfused at the desired pressure.
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated with Alexa Fluor 488-conjugated wheat germ agglutinin (WGA) lectin (Invitrogen, cat# W11261 ...
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bioRxiv - Cell Biology 2023Quote: EPCR/ICAM1-binding iRBCs were obtained via magnetic enrichment of dual-binding iRBCs with ProteinA-coupled Dynabeads (Invitrogen), which were associated to anti-EPCR/ICAM1-binding PfEMP1 antibodies (a kind gift from Anja Jensen ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... IPS cells were thawed and cultured at all times cultured on recombinant human laminin LN521 (Biolamina, 2021-21) kept in E8 basal medium (Thermo Fisher Scientific) supplemented with primocin (0.1 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
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bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...
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bioRxiv - Cell Biology 2020Quote: ... 0.055 mM β-mercaptoethanol (#21985-023) and recombinant human full-length bFGF (#PHG0261) at 10 ng/ml (all reagents from Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Cell Biology 2023Quote: ... DD5A) and three controls (Ctl1B, Ctl7C, Ctl8F) were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... low-binding culture plate (Thermo Fisher Scientific) for 2 days in GMEM supplemented with 15% Knockout serum replacement (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... High-binding 96-well ELISA plates (Nunc) were coated with anti-mouse IL-10 (eBioscience ...
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bioRxiv - Microbiology 2020Quote: High-binding 96-well ELISA plates (Nunc) were coated with 0.5 µg/well of purified SARS-CoV-2 N protein in carbonate/bicarbonate buffer 0.05 M pH 9.6 and allowed to bind over night at 4°C ...
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bioRxiv - Microbiology 2021Quote: High binding capacity 96-well plates (Nunc) were washed and coated with 50 μl per well of 2μg/ml of recombinant spike protein (Sino Biological ...
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bioRxiv - Immunology 2020Quote: ... high-binding 96-well microtiter plates (Nunc) were coated overnight at 4°C with Neutravidin (2 μg/ml in PBS).
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bioRxiv - Microbiology 2020Quote: ... High-binding 96-well plates (Nunc Maxisorp) were coated with 50 µl per well of 2 µg/ml Spike trimer ...
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bioRxiv - Immunology 2020Quote: ... High-binding 96-well microtiter plates (Nunc) were coated overnight at 4°C with Neutravidin ...
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bioRxiv - Immunology 2019Quote: MaxiSorp high-binding 96-well plates (Nunc) were coated overnight at 4°C with 100 µL/well of 10 µg/mL goat anti-mouse IgA antibody (Bethyl Laboratories ...
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bioRxiv - Immunology 2020Quote: ... 96-well high binding plates (Thermo Fisher) were coated with either SARS-CoV or SARS-CoV-2 spike RBD protein (0.1 μg per well ...
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bioRxiv - Cell Biology 2023Quote: ... High protein-binding MaxiSorp plates (ThermoFisher Scientific) were coated with 200 ng/well soluble LCMV sGP resuspended in 1x TBS and incubated overnight at room temperature ...
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bioRxiv - Immunology 2023Quote: ... nickel high binding plates (Thermo Scientific, 15442) were coated with recombinant SARS-CoV2 spike protein (AcroBiosystems ...
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bioRxiv - Microbiology 2023Quote: ... washed with Annexin V binding buffer (ThermoFisher) and spun down at 470g for 5min ...
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bioRxiv - Plant Biology 2024Quote: ... Anti-GFP (Invitrogen, A11122, for LHP1 binding), anti-EIN3 ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Immunology 2021Quote: ... Soluble material was treated with DNase-Free RNaseA (20µg) (Thermo Fisher, ENO531) at 37°C for 1hr ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was incubated with Dynabeads Protein A (Thermo Fisher Scientific), previously coupled with anti-TcSOD-A or anti-TcSir2rp3 antibodies ...