Labshake search
Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Biotin-Maackia Amurensis lectin was labeled with streptavidin-PE conjugate (Thermo Fisher SA10041) and after washing lectin staining was imaged in a Keyence BZ-X700.
-
bioRxiv - Developmental Biology 2022Quote: ... The Alexa Fluor 488 conjugate of lectin PNA (1:400, L21409, Thermo Fisher), the Mito-Tracker Deep Red 633 (1:1000 ...
-
bioRxiv - Neuroscience 2020Quote: ... or streptavidin with Alexa 594 for WFA lectin staining (S11227, Thermo Fisher Scientific), counterstained with 4’,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Synthetic Biology 2021Quote: ... primary microglia were labeled with tomato lectin conjugated to DyLight 649 (ThermoFisher; L32472), which was first subjected to ultrafiltration to reduce the concentration of cytotoxic NaN3 ...
-
bioRxiv - Microbiology 2023Quote: ... FITC-labelled sambucus nigra lectin (10 μg/mL, Thermo Fisher Scientific, 60 min), or mouse IgG1 specific to the ISAV hemagglutinin esterase (clone 3H6F8 [52] ...
-
bioRxiv - Immunology 2023Quote: ... cells were incubated with fluorophore-conjugated lectins diluted in HBSS buffer (ThermoFisher Scientific) for 30 min at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... Pierce™ white streptavidin-coated high binding capacity 96-well binding plates (ThermoFisher Scientific; 15502) were washed twice with ELISA wash buffer and coated with 5μg/ml of biotiylated peptides at room temp ...
-
bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
-
bioRxiv - Biophysics 2020Quote: ... the binding reactions was centrifuged at 1000 RCF for 3 minutes and the pellet was washed with Ag/Ab binding buffer (Thermo Scientific) three times ...
-
bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... a soluble dye that cannot cross membranes (Thermo Fisher Scientific). Subsequently ...
-
bioRxiv - Physiology 2023Quote: ... Soluble molecules were quantified using ProcartaPlex multiplex immunoassay kits (Invitrogen) according to manufacturer instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Soluble fractions were combined with Protein G-Dynabeads (Thermo Fisher) that had been pre-incubated with mouse anti-V5 antibody (2 µg ...
-
bioRxiv - Bioengineering 2023Quote: ... was mixed with 77 mg of water-soluble probenecid (Invitrogen) to obtain a 250 mM solution of probenecid and the mixture was agitated in a vortex until dissolved ...
-
bioRxiv - Cell Biology 2023Quote: ... The soluble fraction was run on a NuPageTM (Thermo Fisher) Tris-Acetate gel and transferred onto a nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
-
bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
-
bioRxiv - Cell Biology 2021Quote: ... or si-hsa-CHST15_0003 (n□=□3) or si-hsa-TNFRSF21_0001 (n□=□3) for 24□hours was carried out by using human Clariom™ Sassay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA ...
-
bioRxiv - Cell Biology 2023Quote: ... or Stealth siRNAs targeting coding sequences of human EZH2mRNA (5′-GACCACAGUGUUACCAGCAUUUGGA-3′: EZH2 #1, and 5′-GAGCAAAGCUUACACUCCUUUCAUA-3′: EZH2 #2) were obtained from Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and re-suspended at a density of 104 cells/mL in pre-warmed complete minimum essential media (MEM) that contains 5 mg/mL human recombinant insulin (Gibco), 1X MEM non-essential amino acids (NEAA ...
-
bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
-
bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
-
bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
-
Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
-
bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
-
bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
-
bioRxiv - Biochemistry 2022Quote: ... and recombinant human Interleukin-2 (rhIL-2, 100 units/mL)) in the presence of anti-CD3/CD28 Dynabeads (Fisher Scientific) at a bead:cell ratio of 1:1 ...
-
bioRxiv - Bioengineering 2022Quote: The human embryonic stem cell line H9 (WiCell, authenticated at source) was grown on recombinant human vitronectin (Thermo) coated cell culture dishes in StemFlex Medium (Gibco). Subculturing was performed by incubating plates with 0.5 mM EDTA for 5 minutes and then resuspended in culture medium to be transferred to new coated plates.
-
bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
-
bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
-
bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
-
bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
-
bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
-
bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
-
bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
-
bioRxiv - Systems Biology 2022Quote: ... and recombinant synthetic genes fused to the GFP-coding sequence at the 3-termini were ordered from GeneArt (ThermoFisher). Genes were further cloned into pDUAL-PuroR lentivectors (Escors et al. ...
-
bioRxiv - Immunology 2023Quote: ... Ganglioside M1 (GM1) staining was performed using recombinant Choleratoxin B labelled with AlexaFluor647 (3 µg/ml, Thermo Fisher, #C34778). Both GFP-D4 and AlexaFluor647-labelled CTB were added to FACS buffer-washed cells and incubated for 20 minutes at 4°C in the dark ...
-
bioRxiv - Microbiology 2020Quote: ... human lung adenocarcinoma Calu-3 cells (ATCC; HTB-55) in MEM (Gibco; #11095-080) containing 10% FBS ...
-
bioRxiv - Immunology 2023Quote: ... primary human foreskin fibroblasts (HFFs) in 3 mL of DMEM (Thermo Fisher Scientific, 11965118) +10% FBS ...
-
bioRxiv - Immunology 2021Quote: ... High-binding 96-well plates (Nunc) were coated with the appropriate antigen (S1 protein 1.2 μg/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... in Annexin V binding buffer (Invitrogen) at a concentration of 5 × 106 cells/mL for 15 min at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... High-binding ELISA plates (Thermo Scientific) were coated with capture antibody and incubated overnight at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... Low-binding tubes (Thermo Fisher Scientific) were used during the whole process ...
-
bioRxiv - Cell Biology 2019Quote: ... in low binding tubes (Life Technologies) and rotated overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: High binding Immunlon HBX plates (ThermoFisher) were coated with 2.5μg/ml of recombinant P ...