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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) plus 2% penicillin-streptomycin (Gibco) at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Microbiology 2020Quote: ... the midguts were further incubated with Alex 647 lectin HPA (Invitrogen, L32454) or Dylight 647 phalloidin (Yeasen ...
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bioRxiv - Biophysics 2020Quote: ... The lectin incubated PS beads were diluted with 100 μl DMEM (GIBCO) buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated for 2h in WGA-Alexa 488 lectin (Invitrogen, Carlsbad, CA, USA), and washed and mounted in anti-fade reagent ...
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bioRxiv - Physiology 2019Quote: ... and IB4 Lectin conjugated to Alexa Flour 488 (1:200, ThermoFisher 121411). Samples were washed with 0.2% saponin in PBS (Sigma-Aldrich S7900 ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotinylated lectins were incubated with streptavidin-coupled magnetic beads (Thermo Fisher Scientific) for 45 min on the overhead shaker ...
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bioRxiv - Neuroscience 2024Quote: ... DyLight 649 conjugated Lectin from Lycopersicon Esculentum (1:400; ThermoFisher Scientific: L32472); Donkey anti mouse IgG (1:600 ...
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bioRxiv - Neuroscience 2022Quote: Total protein content in the PBS-soluble (clarified 10% brain homogenate) and detergent-soluble fractions was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: ... Soluble RBD protein was coated on Maxisorp plates (Nunc) with 2μg/ml of purified protein and incubated with three-fold serially diluted sera (with 1:5 as starting dilution ...
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (CD28.2, 1 μg/ml, Thermofisher), followed by cell staining at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... and Aniline Blue soluble sodium salt (10656822; ThermoFisher, France), were used at concentrations of 25 µg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... Soluble protein was quantified by Pierce BCA assay (ThermoFisher) and stored in 50% glycerol at -80 °C.
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Physiology 2021Quote: ... and non-immunological binding of the specific immune sera were blocked by 3% bovine serum albumin (Thermo Fisher Scientific Inc.). The indirect method was introduced to immunohistological staining ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Cancer Biology 2019Quote: ... and labeled on ice with 1:250 dilution of secondary antibodies for binding (anti-mouse 488, ThermoFisher A11059 or anti-human 647, ThermoFisher A21445) and expression (anti-chicken 647 ...
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bioRxiv - Immunology 2023Quote: ... and processed for quantitative PCR analysis of SMAD3 and SP1 binding to the human MCEMP1 promoter using Power SYBR Green PCR Master Mix (ThermoFisher Scientific). ChIP-qPCR results were calculated using the ΔΔCt method and are shown as percentage of input DNA for each ChIP experiment.
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bioRxiv - Genomics 2021Quote: ... using Lysis/Binding Buffer (Invitrogen), and frozen at −80◦ ...
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bioRxiv - Immunology 2023Quote: ... protein-binding plates (Nunc Maxisorp) were coated with rMOG (housemade ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Cell Biology 2023Quote: ... custom micropatterned glass coverslips presenting 500 µm groWTh areas (CYTOOCHIPS Arena 500A) were covered with 800µL of 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were grown in glass surfaces previously coated with 10μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and seeded onto new 6-well plates at 2,500 cells/cm2 with 1.5 μg/cm2 of truncated recombinant human vitronectin (Vtn-N; ThermoFisher #A14700). After allowing cells to adhere overnight ...
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bioRxiv - Genomics 2023Quote: ... and then at time 0 media was exchanged for complete HaCat media + 20ng/mL human recombinant EGF (Gibco PHG0311L) for all wells except the 0hr timepoint ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Biochemistry 2024Quote: ... The human Dicer/TRBP enzyme complex was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM HEPES pH = 7.5 ...
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bioRxiv - Cell Biology 2024Quote: IMR90 fibroblasts were differentiated to myofibroblasts with 5ng/mL Recombinant Human Transforming Growth Factor -β1 (TGF-β1) (Invitrogen, #PHG9204) for 48h in serum free media ...
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bioRxiv - Cell Biology 2023Quote: ... MiniBAR-GFP sta-ble cell line was transfected with 25 nM of siRNAs targeting luciferase (5’-CGUACGCGGAAUACUUCGA-3’) or human Rab35 (5’-GCUCACGAAGAACAGUAAA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...