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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... was added to the soluble fraction and incubated with 50 μl Protein A Dynabeads (Invitrogen) bound to 5 μg of BRD4 antibody (Bethyl A301-985A50 ...
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bioRxiv - Biochemistry 2023Quote: ... Total soluble protein concentration in supernatant was determined with BCA assay (Thermo Scientific, Pierce 23225). Denatured gel was run using a Thermofisher Scientific Mini Gel Tank system with Bolt Bis-Tris 8% or 4-12% gels and Bolt MES or MOPS running buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the soluble protein fraction was collected and quantified using the BCA assay kit (Fisher Scientific). For each sample ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in the soluble extract was quantified by using the BCA method (Thermo Scientific). For post-mortem material from FTD patients ...
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bioRxiv - Cell Biology 2020Quote: ... Plasma membrane and Golgi apparatus of transfected cells were stained with 0.5μg/ml lectin-HPA conjugated to Alexa Fluor 647 in PBS supplemented with 1% BSA (Life Technologies) and washed twice with PBS prior imaging ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were incubated with peanut lectin (PNA) conjugated to Alexa Fluor 647 (L32460, Thermo Fisher Scientific, 1:2,000) and DAPI (340-07971 ...
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bioRxiv - Molecular Biology 2020Quote: ... tomato lectin was visualized with 2 μg/ml streptavidin conjugated to either Texas Red or Alexa Fluor 488 (ThermoFisher). Dye-conjugated secondary antibodies (Southern Biotechnology ...
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bioRxiv - Developmental Biology 2022Quote: ... Acrosomes were visualized using 0.5 µg ml-1 lectin peanut agglutinin (PNA) Alexa Fluor 488 conjugate (L21409, Life Technologies). Sections were mounted under Dako fluorescence mounting medium and glass coverslips (GM304 ...
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bioRxiv - Cell Biology 2023Quote: ... and lectin microarrays) were carried out in the same mediums with 5% dialyzed Fetal Bovine Serum (FBS) (Gibco, A3382001) replacing the standard HS used for MCF10A culture ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/mL recombinant murine EGF (Gibco, PMG8043), 100ng/mL recombinant human Noggin (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/ml recombinant EGF (Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant ORF8 (ThermoFisher, Cat. RP-87666) was conjugated with FITC using the FITC conjugation kit (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) was added to lysate (1ul per 100ul lysate) ...
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bioRxiv - Genomics 2019Quote: ... 0.25 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Cas9 2.0 (Thermo Fisher Scientific, A36498) was mixed with gRNA and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant LRRK2 protein was from ThermoFisher (#A15197). For pT73 Rab10 assay ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). The transfection of primary NHKs to generate HPV18+ keratinocytes was performed as described previously [86] ...
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bioRxiv - Molecular Biology 2022Quote: ... RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) and Protoscript II reverse transcriptase (New England BioLabs ...
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bioRxiv - Physiology 2022Quote: ... Recombinant PIK3CG was purchased from Thermo Fisher.
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant TrueCut Cas9 V2 (Thermo Fisher Scientific) was diluted in resuspension buffer R ...
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bioRxiv - Immunology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (10777019, Invitrogen); Deoxynucleotide (dNTP ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). HPV18+ NHKs were generated as described previously [86] ...
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bioRxiv - Cell Biology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher), Random Hexamer Primers (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Neuroscience 2021Quote: ... streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... and binding revealed using SuperSignal West Pico Plus reagent (ThermoFisher) was used as chemiluminescent substrate ...
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bioRxiv - Cell Biology 2022Quote: ... in 100 μl of 1X Annexin Binding Buffer (Thermo Fisher) for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... High-binding 96-well plates (Nunc MaxisorpTM, Thermo Fisher Scientific) were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck) ...
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bioRxiv - Immunology 2021Quote: ... High-binding 96-well plates (Nunc MaxisorpTM, Thermo Fisher Scientific) were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck) ...
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bioRxiv - Molecular Biology 2019Quote: ... Following binding of AlexaFluor 488-conjugated secondary antibody (Thermo Fisher) and nuclear staining with Hoechst (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: Binding assays were performed in 96-well plates (Nunc MaxiSorp) coated overnight at 4°C with 100 µL of 5 µM AsaA_NRR ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific binding was blocked with Mouse IgG Isotype Control (Invitrogen) for 25 minutes at 4°C ...
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Complement-associated loss of CA2 inhibitory synapses in the demyelinated hippocampus impairs memorybioRxiv - Neuroscience 2021Quote: ... Streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 96-well Corning Costar high binding plates (Thermo Fisher Scientific) were coated with SARS-CoV-2 Sike protein in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... and stored in lysis/binding buffer (Thermo Fisher Scientific, USA). The messenger RNA (mRNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Nunc Maxisorp high-binding plates were purchased from Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: High-protein binding 96-well microplates (3855, ThermoFisher, MA, US) were coated overnight with 10 µg/ml of either porcine collagen II (20031 ...
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bioRxiv - Molecular Biology 2022Quote: ... Ligand binding SARM1 was incubated with DSBU crosslinker (Thermo Fisher) to a final concentration of 200 µM for 30 min at 25℃ ...
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bioRxiv - Physiology 2022Quote: ... and ionized calcium binding adaptor molecule 1 (IBA1; Invitrogen MA527726) were added at 1:1000 and incubated at 4°C overnight in a humidity chamber ...
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bioRxiv - Immunology 2023Quote: ... for murine and Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen) for human samples and subsequently stained with live/dead cell exclusion dye (Zombie Dyes ...
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bioRxiv - Molecular Biology 2021Quote: ... Then recombinant dsDNA fragment (add to > 400 ng/μl final concentration) or recombinant single-strand DNA (ordered from Invitrogen or IDT ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells or MiniBAR-GFP stable cell lines were transfected with 25 nM of siRNAs targeting either luciferase or human Mini-BAR (5’-CUGCAAAUUUUACGGAUCA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Cell Biology 2023Quote: Monkey kidney epithelial cells Vero E6 (clone CCL81) and human lung epithelial cells Calu-3 were respectively cultured in DMEM medium and EMEM medium (ThermoFisher) supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Microbiology 2021Quote: ... the slides were rinsed with dH2O to remove unbound material and holdfast were visualized with AF conjugated lectins (Molecular Probes). Imaging was performed using an inverted Nikon Ti-E microscope with a Plan Apo 60X objective ...
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bioRxiv - Microbiology 2021Quote: ... ECs were labelled for 10 min at 37 °C with 100 μl of a 1:10,000 dilution of Alexa 647-wheat germ agglutinin (lectin) (Life Technologies) to label the glycocalyx (GX ...
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bioRxiv - Microbiology 2019Quote: ... mixed with 50 μl of a 0.1 mg/ml Wheat Germ Agglutinin (WGA)-Alexa Fluor® 594 conjugate lectin solution (Invitrogen) and incubated for 5 min at room temperature ...