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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... infected cells were stained with primary antibody (Vaccinia Virus Polyclonal FITC Antibody ThermoFisher PA1-73191, 1:1000) and Hoechst 33342 (1:10,000) ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... Lysates were then incubated for one hour at 4°C with Dynabeads-Protein A or Dynabeads-Protein G (ThermoFisher) that had been bound to anti-GFP (clone 3E6 ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were denatured at 95°C for 10min and protein quantity was measured using Pierce 660nm Protein Assay (ThermoFisher). Protein extracts (10µg ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were clarified by centrifugation at 4°C and the protein concentration was determined by BCA protein assay (Thermofisher). Samples were resolved on a precast 4%–12% gradient gel (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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bioRxiv - Genetics 2021Quote: ... DSF was performed by heating samples containing 10X SYPRO Orange in 1°C increments from 25°C to 100°C using the StepOne real-time PCR machine and using the Protein Thermal Shift software (ThermoFisher) to fit the data.
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xCENP-C mutants in interphase CENP-C-depleted extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL Myc-xCENP-C truncations in interphase CENP-C-depleted extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-Myc antibody ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... 40uL of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) were added to each well and then incubated at 4ºC for 4 hours on a rotator ...
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bioRxiv - Genomics 2019Quote: ... 110μL of a 1:1 Protein A and Protein G Dynabead slurry (ThermoFisher Scientific) was prepared by combining equal volumes of the Protein A and G beads and washing twice in low salt RIPA buffer with SDS (140mM NaCl ...
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bioRxiv - Systems Biology 2020Quote: ... human buffy coat samples were diluted 1:1 in PBS (Gibco) and mononuclear cells were isolated with a Histopaque-1077 density gradient (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal rabbit anti-mouse/human ZO-1 (1:200; Life Technologies), monoclonal rat anti-mouse ICAM-1 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... HIV-1gag/pol and pWPI-bla-IRF1 into HEK293T cells was performed using Lipofectamine 2000 (Invitrogen). Supernatants containing lentiviral pseudoparticles for transgene delivery were harvested at 24 h and 48h ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1JR-FL-infected primary CD4+ T cells were stained with viability (AquaVivid; Thermo Fisher Scientific) and cellular (cell proliferation dye eFluor670 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured on 6-well plates coated with Vitronectin Recombinant Human Protein (Invitrogen) at a concentration of 0.5 µg/cm2 ...
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bioRxiv - Microbiology 2021Quote: ... For samples that were mixed with protein (BSA, human CAR, mouse CAR [Fisher Scientific]) prior to attachment ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... and the human angiopoietin-like 4 protein (ANGPTL4) ELISA Kit were purchased from Invitrogen-ThermoFisher Scientific (Carlsbad ...
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bioRxiv - Immunology 2022Quote: ... Proteins were then produced in human embryonic kidney (HEK)293F cells (Thermo Fisher Scientific) maintained in Freestyle medium (Life Technologies) ...
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bioRxiv - Neuroscience 2023Quote: Human fibroblasts were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with cOmplete protease and PhosSTOP phosphatase inhibitors (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% human keratinocyte growth supplement (Gibco), 0.2% GibcoAmphotericin B (250 µg/mL of Amphotericin B and 205 µg/mL sodium deoxycholate ...
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bioRxiv - Cell Biology 2022Quote: ... 1% P/S and human TLR agonists (InvitroGen) in 96-well round bottom plates. ...
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bioRxiv - Immunology 2019Quote: ... anti-human CD45RO (UCHL-1, Thermo Fisher Scientific), anti-mouse CD3 (17A2 ...
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bioRxiv - Neuroscience 2022Quote: ... 10μg ml−1 human recombinant insulin (GIBCO, 12585014).
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bioRxiv - Neuroscience 2023Quote: ... Human tau-specific (HT7, 1:2,000; Invitrogen MN1000) and TaupThr231/pSer235(AT180 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% Human Keratinocyte Growth Supplement (#S0015 Gibco), 1% Pen/Strep and 2.5 µg/mL amphotericin B ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human PSGL-1-PE (TC2, Life Technologies) at 1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-human FRα (1:1000 pH9, Thermofisher).
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Molecular Biology 2022Quote: ... cyt-c-p (1:100, incubated overnight at 4°C, Thermo Fisher Scientific). All biological replicates were tested by western blotting.
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bioRxiv - Physiology 2022Quote: ... C-IV-II and C-V-alpha (ThermoFisher Scientific, 45-8199; 1:2500). Secondary antibodies used were horseradish peroxidase–conjugated anti-mouse (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Molecular Biology 2021Quote: Human erythroleukemia K562 cells were grown at 37°C at 5% CO2 in RPMI 1640 (Gibco) with 10% FBS (Sigma ...
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bioRxiv - Biochemistry 2021Quote: Human HEK293S GnTI- cells were cultured as suspension at 37°C in FreeStyle293 expression medium (Invitrogen) supplemented with 2% (vol/vol ...
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bioRxiv - Biophysics 2023Quote: Human neuroblastoma cell line (SK-N-BE) stored at -80°C in freezing medium (RPMI Gibco® supplemented with 20% FBS Gibco and 10% DMSO) ...
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bioRxiv - Physiology 2023Quote: ... anti-human HLA-A,B,C to detect leukocyte derived EVs and Annexin V (ThermoFisher, UK), which binds externalized phosphatidylserine (PS ...
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bioRxiv - Microbiology 2020Quote: ... the primary substrates were 24.7 ± 0.5 mM fructose (148.2 ± 3.2 mmol C L−1) and 18.7 ± 1.0 mM Na-butyrate (112.2 ± 6.3 mmol C L−1) (ThermoFisher, Kandel, Germany). For the pH experiment ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C and total protein determined by the BCA method (Thermo Fisher Scientific). Proteins were separated on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... was carried out overnight at 4°C using Protein A Dynabeads (ThermoFisher Scientific). The mononucleosomal fraction (147 bp ...
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bioRxiv - Bioengineering 2023Quote: ... protein expression was induced overnight at 16°C with 0.2mM IPTG (Thermofisher, 15529019). The overnight cultures were lysed and sonicated in non-denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and protein concentrations were quantified using a CBQCA Quantification kit (C-6667, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted by incubation at 95°C in NuPAGE Loading Buffer (Invitrogen) for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were expressed overnight at 18°C in Terrific Broth (Thermo Scientific) and 0.4% v/v glycerol with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG ...