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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitations were performed overnight at 4°C with Dynabeads Protein G beads (Invitrogen) conjugated to mouse anti-FLAG M2 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Systems Biology 2023Quote: ... EBs were transferred in 24 well Ultra Low Attachment plates (Thermo Fisher Scientific P24; ref. 144530 “ untreated Nunc”) with EB Formation Medium (same as above except that ROCK Inhibitor is used at 20 µM) ...
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bioRxiv - Systems Biology 2022Quote: ... cytochrome C (1:200, Invitrogen, Carlsbad, CA), pRb (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... c-myc (Invitrogen 13-2500, 1: 1,000), CCT2 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... or 1 µg Lys-C (Thermo Scientific) were added to the columns and incubated at 37 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were then clarified by spinning at 14,000 rpm at 4°C and protein concentration quantified by BCA Protein Assay (23227, Thermo Scientific). Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093 ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Immuno-precipitation was done overnight at 4°C using Protein A-coupled or Protein G-coupled Dynabeads previously incubated with anti-GFP A11122 antibody (Invitrogen), anti-Flag antibody (M2 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble fraction was removed via centrifugation (13,000xg, 10 min, 4 °C) and protein abundance was quantified by BCA Protein Assay (Thermo Scientific). 50 μg of protein was utilized per well in a total volume of 60 μL ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Ago2 and IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A or G (ThermoFisher Scientific). After incubation ...
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bioRxiv - Genetics 2019Quote: Coverslips were incubated for one hour at 37°C in 1mL of of 1:30 solution of Collagen I Rat Protein (Thermo Fisher Scientific) to 1X PBS ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was incubated at 4°C for 1 h with anti-BmAgo3 antibody23 conjugated to Dynabeads protein G (Thermo Fisher Scientific). The superparamagnetic beads were washed five times with wash buffer (30 mM HEPES-KOH ...
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bioRxiv - Immunology 2022Quote: Plasma was pooled and heat inactivated at 56°C for 1 hour then diluted in protein G binding buffer (Pierce ThermoFisher, catalog #54200) and passed over a column containing 1mL of protein A/G resin (Pierce ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were denatured for 1 minute at 70°C and RNA-protein complexes were resolved on NuPAGE 4-12% Bis-Tris Gels (Thermo Fisher, #WG1402A) at 180V for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary (4°C overnight) and secondary antibodies (1 h, room temperature) dilutions and proteins were visualized with enhanced chemiluminescence (ECL) (Pierce; Thermo Fisher Scientific). For loading control (Supplementary figures 3b and 3k) ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained with primary antibodies overnight at 4°C targeting the following proteins: MAP2 (PA1-10005, ThermoFisher Scientific, 1:5000), AT8 (MN1020 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at 4°C before the addition of 25 μL of Pierce Protein A/G Magnetic Beads (Thermo Fisher Scientific) for an additional 2 h under constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
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bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...
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bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Genomics 2022Quote: ... 15 μl of a 1:1 mix of Protein A and Protein G Dynabead (Invitrogen) was added followed by a 90 minute incubation at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 80,000 M-1·cm-1) against the protein concentration determined using Bradford protein assay (ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified via qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) on a QuantStudio 3 (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...