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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Pure cultures of 10 × 104 prostate stromal cells seeded on twelve well plates were transduced using Cytotune 2.0 Sendai Virus reprogramming vectors (KOS, c-Myc and Klf4, Life Technologies) at a multiplicity of infection of 5-5-3 (KOS MOI=5 ...
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Genetics 2019Quote: ... human COT-1 DNA (Invitrogen) and biotin-labeled mouse COT-1 or minor satellite DNA (a gift from Dr ...
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bioRxiv - Genetics 2021Quote: ... human COT-1 DNA (ThermoFisher) to detect HSA21 and biotin-labeled I-EGFP-I-loxP-3’HPRT (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Human Cot-1 DNA (Invitrogen), 3M NaAc and 100% cold EtOH ...
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bioRxiv - Biochemistry 2020Quote: ... We measured packaging of the RNA from an HIV-1-derived GFP vector constructed from pLenti6/V5-DEST (ThermoFisher, Inc.). A Psi-version of this vector was constructed ...
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bioRxiv - Microbiology 2021Quote: ... HIV-1NL4-3ΔEnv-eGFPΔNefΔvpu or HIV-1 NL4-3ΔEnv-eGFPΔNef viral supernatants or infection media control (DMEM with glutamax (Gibco Cat# 10566-016) with 10% FBS ...
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bioRxiv - Immunology 2019Quote: ... The gag-protease region was amplified by reverse transcription (RT)-PCR from plasma HIV-1 RNA using Superscript III One-Step Reverse Transcriptase kit (Invitrogen) and the following gag-protease -specific primers ...
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bioRxiv - Molecular Biology 2021Quote: ... then used for qPCR analysis using primers that amplify U5-R on HIV-1 in a SYBR green master mix (ThermoFisher). ΔΔCt was calculated relative to total histone H3 levels and expressed as a fold change relative to CEM-SS cells not expressing Tax and infected with WT HIV-1.
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bioRxiv - Immunology 2022Quote: HEK 293T were co-transfected with pmCherry/ pmCherry-SLFN11/ pmCherry-SLFN12 (12 μg) and HIV-1-pNL-E (12 μg) using Lipofectamine 2000 (48 μl) in a T-150 culture dish (ThermoFisher). In order to freeze elongation ribosomes ...
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bioRxiv - Cell Biology 2023Quote: ... The following Tat coding sequences (based on the molecular HIV clone NL4.3) were introduced into the DNA expression vector pVax-1 (Life Technologies): tat86 (86 referring to length in amino acids ...
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bioRxiv - Microbiology 2023Quote: ... Viral RNA producing cells were detected by labelling HIV-1 unspliced mRNA with a set of 40 probe pairs against the GagPol region (Affymetrix eBioscience catalogue number GagPol HIV-1 VF10-10884 ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 37°C to OD600 0.6 and protein expression induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; ThermoFisher Scientific) for 4 hr at 30°C (3CL-pro and RBD) ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
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bioRxiv - Neuroscience 2023Quote: ... The total protein concentration was determined by BCA assay and 500 μg of total proteins was incubated for 1 hour (4°C, rotary agitation) with 25 μl of Pierce™ Protein A Agarose beads (ThermoFisher, cat. no. 20333), centrifuged at 2500 g for 3 minutes at 4°C and the supernatant recovered as pre-cleared lysate ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Neuroscience 2020Quote: Levels of total APOE protein were analysed by Human ELISA Kit from Invitrogen (Apolipoprotein E Human ELISA Kit). Levels of Aβ were analysed by ELISA (Human β Amyloid 42 ELISA Kit Wako high sensitive #298-64401 and Human β Amyloid 40 ELISA Kit Wako #298-64601 ...
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bioRxiv - Developmental Biology 2020Quote: Islets from 3 control and 3 Dpy30ΔN male mice were isolated at P24 and 100-200 islets per mouse were handpicked into 1 mL of TRIzol reagent (Thermo Fisher Scientific). Extracted RNA was treated with TURBO DNase I (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... was reverse transcribed at 37°C for 50 min in a 20 µl reaction mix containing 200U Moloney murine leukemia virus (MMLV) reverse transcriptase (Life Technologies), 100 ng random primers ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 2.5 μg / 500 mL EGF recombinant human protein (Gibco) and 1% penicillin and streptomycin (Wisent ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng/mL human EGF recombinant protein (Life Technologies, Cat: PHG0311), and 100 IU/mL P/S ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/uL human IL-6 recombinant protein (ThermoFisher PHC0066).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... VEGF ligand (recombinant human protein) was ordered from ThermoFisher (CAT: #PHC9391).
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bioRxiv - Bioengineering 2024Quote: ... 5250 ng/mL human recombinant IL-6 protein (Invitrogen; Waltham, MA) was added to the experimental group and an equal volume of 1X PBS was added to the control group ...
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bioRxiv - Cell Biology 2024Quote: ... Immunofluorescence (IF) staining was performed with antibodies for prosurfactant protein-C (proSP-C) (Invitrogen, PA5-71680), NKX2.1 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed using TaqMan Fast virus 1 Step PCR Master Mix (Life Technologies), standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... amplified using the TaqMan Fast Virus 1-Step Master Mix qRT-PCR kit (Invitrogen) on a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... probes and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA). We used pre-combined probe and primers (500 nM primers and 250 nM probe ...
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bioRxiv - Microbiology 2021Quote: ... while 4x Taqman Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA) was used for RNA enteric viruses ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNA was quantified using qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Immunology 2022Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows:
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix (Cat. n° 4444434, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... were quantified by RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and specific primers and probes (Supplementary Table 3) ...
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bioRxiv - Immunology 2019Quote: ... and inoculated with virus at the optimal MOI of 100:1 in DMEM (Gibco). After 1.5 h ...
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bioRxiv - Zoology 2020Quote: ... 6.25μL of 4X RT-Buffer (TaqMan Fast Virus 1-Step Master Mix, ThermoFisher Scientific), 600nM of the forward primer (RdRp_SARSr-F) ...
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bioRxiv - Microbiology 2021Quote: ... Eluted RNA was coupled with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and nCOV_N1 primers/probe (IDT ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A Virus M1 (1:1,000; clone: GA2B; Cat#: MA1-80736; Invitrogen), mouse anti-Influenza A virus M2 (1:1,000 ...
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bioRxiv - Bioengineering 2022Quote: ... consisting of 5 μL TaqMan Fast Virus 1-Step Master Mix (cat# 4444432, Thermofisher), 1.2 μL forward primer (0.6 μm) ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A virus M2 (1:1,000; clone: 14C2; Cat#: MA1-082; Invitrogen), mouse anti-GAPDH (1:1,000 ...