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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/ml bFGF recombinant human protein (Thermo Fisher Scientific, USA). From day 6 until day 12 the medium was changed once in two days with the addition of 1 mM valproic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Human recombinant epidermal growth factor (hEGF) protein was purchased from Life Technologies and dissolved in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Immunology 2021Quote: ... The phage/serum mixtures were rotated overnight at 4°C after which 40 μL of a 1:1 Protein A/G coated magnetic bead mixture (Invitrogen, #10002D and #10004D) was added to each well and rotated for an additional 4 hours at 4°C to capture all IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubes were incubated for 1 hour at 4°C and then protein A/G agarose magnetic beads (Thermo Scientific) were added for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were precleared by incubation for 1 hour at 4°C on a rotator with 10μl Protein G magnetic beads (ThermoFisher). The indicated antibody was incubated in 600μl IP buffer containing 1μl 10mg/ml acetylated BSA and 5μl Protein G magnetic beads for 4 hours with rotation at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with either horseradish peroxidase (HRP)-conjugated anti-c-myc antibody (1:5,000) (Thermo Fisher Scientific) or HRP-conjugated anti-GFP antibody (1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed at 4 °C for Scribble and CHIKV proteins using anti-Scrib (1:500, Invitrogen PA5-54821), anti-E2 (1:500 ...
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bioRxiv - Immunology 2020Quote: ... luciferase-expressing HIV vector (pNL4–3.luc.RE) using Lipofectamine 2000 reagents (Life Technologies, USA). After 48 h ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Pelleted virus was resolved in PBS (Gibco) and stored at −80°C until further usage ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and EmGFP reporter Sendai Virus (A16519, Invitrogen) were part of the Cytotune -iPS 2.0 Sendai Reprogramming kit and EmGFP Sendai Fluorescence Reporter kit ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Cell Biology 2019Quote: All human cells (listed in table 1) were cultured at 37°C in an atmosphere of 5% CO2 in DMEM (Thermo Fisher Scientific) supplemented with penicillin/streptomycin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Cell Biology 2021Quote: ... S1 C (Pierce Unstained Protein MW Marker from Thermo Scientific, 26610) and Fig ...
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bioRxiv - Biochemistry 2021Quote: ... immobilized on Dynabeads Protein A magnetic beads (Thermofisher, c/n 10001D) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were immobilized overnight at 4°C on Maxisorb™ (Nunc) 96 wells microplates at 1µg/well in 100 µl NaHCO3 0.1 M pH 8.9 ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... isolated with Protein A Dynabeads (Invitrogen; 4 h at 4°C), washed thrice with RSB+T ...
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bioRxiv - Genetics 2019Quote: ... and 5 µl of protein A: protein G 1:1 Dynabeads (Thermofisher). Antibody-bond chromatin was washed four times in low salt buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... A 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was prepared and 40 μL of the mixture was added to each well ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Genomics 2020Quote: ... and subsequently subjected into nested polymerase chain reactions (PCR) according to manufacturer’s instructions prescribed in HIV-1 genotyping kit: Amplification module (Applied Biosystems, Life Technologies, Warrington, UK). The amplified cDNA was purified using ExoSAP-IT™ PCR product clean-up reagent (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Fragments hybridizing to biotin-labelled HIV-1 DNA were pulled down by incubating the hybridization reaction with streptavidin-conjugated magnetic beads (ThermoFisher Scientific, Cat#88816) at room temperature for 30 min ...
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bioRxiv - Pathology 2023Quote: Total DNA was then extracted from HIV-1 infected cells at 6 h post-infection using the PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific). Quantitative PCR for R-U5 and LTR-Gag transcripts was performed using the following primers:
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1% human keratinocyte growth supplement (HKGS, Gibco) and 1% pen-strep ...
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bioRxiv - Immunology 2022Quote: ... Sheep anti-human FH (1:200×, ThermoFisher) and then goat anti-sheep HRP (1:2000× ...
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bioRxiv - Cell Biology 2020Quote: ... Human Cot-1 (DNA) (Thermo Fisher Scientific) (10 μg per 2 μg of nick translated BAC vector ...
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bioRxiv - Neuroscience 2023Quote: ... anti-human IgG (Invitrogen, A21445, 1:400).
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bioRxiv - Cell Biology 2023Quote: ... or human (1:2000; Thermo Fisher Scientific). DAPI was used to stain DNA (500 ng/mL ...
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bioRxiv - Microbiology 2023Quote: ... 1:2500 goat anti-human Ab (Invitrogen), 1:5000 goat anti-rabbit Ab (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... 1:1000 anti-human-AF594 (Molecular Probes) and 1:2000 L/D eFluor506 (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Catalog Numbers 4444432). Briefly ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... Extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen) with oligo(dT ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Taqman Fast Virus 1-step Master Mix (Thermo Fisher Scientific, 4444434) in StepOne Plus real-time PCR thermocycler (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... vRNA was then reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Vero cells were infected with passage 9 virus and overlaid with 1% low melting agarose (Invitrogen) in MEM ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was conducted using TaqMan Fast Virus 1-Step Master Mix from Applied Biosystems in 384-well plates ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM PMSF and pre-cleared for 2h at 4°C by adding magnetic Protein G Dynabeads (Life Technologies). Beads were discarded and 1 µg of anti-CTCF antibody (Diagenode #C15410210 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...