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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Each extracellular protein fraction (#1-15) collected by UF-SEC was collected and protein concentration determined by Micro BCA assay (Thermo Fisher Scientific) as according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Physiology 2023Quote: At day 45 (± 2 days) protein was extracted from beating iPSC-CMs in protein lysis buffer (1 X Pierce™ RIPA buffer (Thermo Fisher); 1 X PhosSTOP cocktail (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... the membrane preparations were solubilized with 1% DDM and the total protein content was measured using Pierce™ 660 nm Protein Assay Reagent (Thermo Scientific) against the BSA standard curve (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Immunology 2019Quote: ... containing LCMV peptides (1:1000, Peptide 2.0 Inc.) and Protein Transport Inhibitor Cocktail (1:500, Thermo Fisher Scientific). Cells were incubated for 4 hours at 37°C and then surface antigens were stained as described above ...
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bioRxiv - Bioengineering 2019Quote: ... The protein concentration of Matrigel was adjusted by dilution with 1:1 DMEM:F12 medium (Life Technologies, Carlsbad, CA). The molecular crowders ...
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bioRxiv - Developmental Biology 2019Quote: ... Chromatin was pre-cleared with 5 or 10 µl of 1:1 protein A:G Dynabeads (Life Technologies #10015D) and immunoprecipitated with H3K4me1 (Abcam #ab8895) ...
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bioRxiv - Cell Biology 2022Quote: Protein was added to 1× NuPAGE LDS Sample Buffer and 1× NuPAGE Reducing Agent (both Thermo Fisher Scientific) in water and incubated at 75°C for 10 minutes for protein denaturation ...
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bioRxiv - Biophysics 2021Quote: ... 10 μl protein (0.15 μg μl−1) with 10x SYPRO Orange dye (Invitrogen) end concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were detected with 1:2,000 diluted anti-GFP (A6455; Thermo Scientific) and 1:1,000 diluted anti-RFP (AB233 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM PMSF) followed by an incubation with antibody/protein A Dynabeads (Invitrogen) complex at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Bioengineering 2021Quote: ... Recombinant protein expression was triggered using 0.5mM isopropyl β-D-1-thiogalactopyranoside (ThermoFisher) and incubated for 18 h in an orbital shaker at 18 °C ...
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bioRxiv - Neuroscience 2021Quote: ... along with a 1:1000 dilution of Sypro Orange Protein Gel Stain (Invitrogen) and a 1:10 dilution of 200 μM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific). On day 4 of differentiation ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 unit of chromatin was immunoprecipitated by protein A magnetic beads (Dynabeads, Invitrogen) after incubation with antibodies specific for HDAC1 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... green fluorescent protein (GFP, conjugated with Alexa 488; rabbit, 1:1000, A21311, Invitrogen; or unconjugated ...
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bioRxiv - Biochemistry 2022Quote: ... Protein cleavage was carried out by addition of 1 μL TEV protease (Invitrogen) to cleave the SMC compartment and 1 μL HRV 3C protease (Pierce ...
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bioRxiv - Biochemistry 2022Quote: ... Protein cleavage was carried out by addition of 1 μl TEV protease (Invitrogen) to cleave the SMC compartment and 1 μl HRV 3C protease (Pierce ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-green fluorescent protein (GFP) (1:500, #A11122, Life Technologies, Waltham, Massachusetts) and rat anti-N-Cadherin (1:50 ...
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bioRxiv - Cell Biology 2019Quote: Mouse TGF-β 1 recombinant protein was obtained from Affymetrix (#14-8342-62) and used at the concentration of 2 ng/ml unless stated otherwise ...
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bioRxiv - Molecular Biology 2019Quote: 30 μg of aggregated proteins in 1 x LDS loading buffer (Life Technologies) supplemented with 100 mM DTT were separated on 4-12% Bis-Tris gels in MOPS running buffer ...
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bioRxiv - Bioengineering 2020Quote: ... and 1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific). On day 4 of differentiation ...
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bioRxiv - Genomics 2021Quote: ... Fifteen µl of Pre-blocked (with 1% BSA) Protein G Dynabeads (10004D, Invitrogen) was added to the tubes and rotated for 4h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... protein was detected using monoclonal mouse Anti-myc (1:7,500; R950-25, Thermofisher), polyclonal rabbit anti-GFP (1:7,500 ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Associated proteins were removed by digesting with 1 mg/ml proteinase K (Invitrogen) for 20 minutes at 37°C in PK buffer (100 mM Tris-HCl pH7.4 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... rabbit anti-phospho-S6 ribosomal protein (Serine244/247) (1:150, Invitrogen, #44-923G), rabbit anti-MS4A1/CD20 (1:250 for immunostaining ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were separated on 1-mm-thick 16% Tricine gels (Life Technologies) for 2 h at 120 V ...
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bioRxiv - Cell Biology 2023Quote: ... Streptavidin-HRP (peroxidase-conjugated streptavidin biotin-binding protein, Thermo Scientific, 21130, 1:10000).
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bioRxiv - Biochemistry 2023Quote: ... and digested with sequencing grade trypsin (∼1:100 enzyme/protein ratio; Thermo Fisher) at 37
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of native marker (Native Marker unstained protein standard, LC0725, Life Technologies) diluted 400 x in the GF buffer was used for mass/contrast calibration that was monitored during 60 s using the AcquireMP software (Refeyn Ltd ...
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bioRxiv - Biophysics 2023Quote: ... protein digests were mixed with 1 mL HisPur Ni-NTA resin (ThermoFisher Scientific) and placed on a rotating mixer maintained at 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were reduced by adding 1 mM DTT (Thermo Fisher Scientific, A39255) and incubating for 20 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... NS1 proteins were detected using DENV2 NS1 polyclonal antibody (1:1,000 ThermoFisher Scientific) with overnight incubation at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... ADH enzyme activity (units: ΔAbs340nm min−1 mL−1) and total protein (units: mg mL−1; Pierce BCA Assay, Thermo Fisher Scientific) of each sample were measured 3 times in technical replicates ...
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Plant Biology 2021Quote: ... The activity of the mitochondrial proteins (1-25 μg protein) were assayed spectrophotometrically using a Multiskan™ GO Microplate Spectrophotometer (Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...