Labshake search
Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
-
Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
-
bioRxiv - Cell Biology 2022Quote: ... The surviving clones were screened for expression of the desired proteins in the presence of 1 μg ml−1 doxycycline (Invitrogen). Expression of Myc-Cdk11 was also induced with 1 μg ml−1 doxycycline in the subsequent experiments.
-
bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
-
bioRxiv - Bioengineering 2021Quote: ... Immunostaining of BBB constructs for tight junction protein ZO-1 was conducted using ZO-1 mouse monoclonal antibody conjugated with Alexa 488 (ThermoFisher). Hydrogels were removed from the transwell insert using a biopsy punch ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 mM TCEP and SYPRO orange protein gel stain at a 1:500 dilution of the manufacturer’s stock (Invitrogen, S6651). The samples also contained 5 μM NBD protein ...
-
bioRxiv - Pathology 2020Quote: ... 1 mM EDTA and 1% SDS) and the protein concentration was quantified by the BCA assay (Thermo Fisher Scientific, #23225). Equal amount of whole cell extracts were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore ...
-
bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
-
bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... chromatin-antibodies complexes were recovered by incubation with 30 μl of Dynabeads® Protein A/G 1:1 mix (ThermoFisher) for 2 h at 4°C under rotation ...
-
bioRxiv - Genomics 2023Quote: ... Sonicated samples were pre-cleared to remove background signal through incubation with a 1:1 mix of Protein A/G Dynabeads (InVitrogen). Antibody was added at a concentration of 1 µg/µL per sample and immunoprecipitated at 4°C overnight ...
-
bioRxiv - Immunology 2024Quote: ... Any bound proteins were eluted by adding a 1:1 mix of 10 mM TBS and LDS buffer (Life Technologies) to the first well and then transferring from well to well ...
-
bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
-
bioRxiv - Immunology 2021Quote: ... a 1 mg/mL solution of these proteins was mixed with the Protein Thermal Shift™ Dye kit (Thermo Fisher, Waltham, MA, USA; Cat # 4461146) in triplicate as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...
-
bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified by BCA Protein Assay (Thermo Scientific) and 50 µg of total protein in 100 mM TEAB and 10% acetonitrile was incubated at 99°C for 1 h.
-
bioRxiv - Genetics 2020Quote: ... protein quantification using BCA Protein Assay Kit (Thermo Fisher) was done ...
-
bioRxiv - Microbiology 2020Quote: ... and proteins were immunoprecipitated with Protein A Dynabeads (ThermoFisher) conjugated to the relevant antibody (anti-MqsRA ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...
-
bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured using BCA Protein Assay (ThermoFisher). For anti-NP immunoprecipitation ...
-
bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with Bradford protein assay (Thermofisher), following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... cas9 protein (Fisher Scientific A36498 Truecut Cas9 Protein v2) and gRNA were co-electroporated into G1 ES cells derived from an F1 hybrid blastocyst of 129S6 x C57BL/6J ...
-
Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... alongside protein standards (SeeBlue Pre-stained Protein Standard, Invitrogen). Gels were run at room temperature for 150 min at 80 V in running buffer ...
-
bioRxiv - Plant Biology 2022Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for 2 hours ...
-
bioRxiv - Plant Biology 2023Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and mixture was rotated at 4°C for 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...
-
bioRxiv - Biochemistry 2023Quote: ... protein quantified by BCA protein assay ( Thermo scientific #23250), and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined by Qubit protein assay (Invitrogen) in a Qubit 4 fluorometer (Invitrogen).
-
bioRxiv - Cell Biology 2023Quote: ... 200 μL of Protein A (Dynabeads Protein A, Invitrogen) beads were washed twice in coIP buffer containing PIs ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
-
bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies included rabbit polyclonal anti-β-COP protein (1:200; Thermo Fisher, PA1-061) and mouse monoclonal anti-flag (1:500 ...
-
bioRxiv - Pathology 2019Quote: ... Proteins were stained with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) until blue colour was fully generated and the reaction stopped by adding 1 M H2SO4 ...
-
bioRxiv - Microbiology 2019Quote: ... Labeled proteins were visualized with Alexa Fluor-conjugated secondary goat antibodies (1:2000, Life Technologies). Nuclei were visualized with the DNA stain (DAPI ...
-
bioRxiv - Cell Biology 2019Quote: ... 100 μM of the protein was incubated with 1 mM tris(2-carboxyethyl)phosphine (Invitrogen) for 30 min at RT protected from light ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 µL of 1M DTT and 2.5 µL of 4x LDS protein loading buffer (Invitrogen) were added to the bead mixture ...
-
bioRxiv - Bioengineering 2021Quote: ... STEMdiff APEL2 medium (1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific) and 1% (v/v ...
-
bioRxiv - Biochemistry 2021Quote: ... The substrate proteins were modified with a 1:0.8 ratio Alexa Fluor 680 (Thermo Fisher) for 10 min and quenched with 5 mM DTT ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were PON1 (18155-1-AP, Protein tech) and PON3 (OTI1A5, Thermo Scientific).
-
bioRxiv - Bioengineering 2020Quote: ... rabbit anti-S100 Protein Ab-2 (S100, 1:500, Thermo Scientific Cat# RB-044-A) was used to identify Schwann cells ...
-
bioRxiv - Microbiology 2021Quote: ... MeV-M proteins were probed using rabbit anti-EGFP polyclonal antibody (1:1000) (Invitrogen #A6455). The Venus fluorescent protein used in BiFC experiments is a genetic variant of EGFP ...
-
bioRxiv - Cell Biology 2021Quote: ... The proteins were probed with the following antibodies: Monoclonal anti-GFP (1:2000, Thermo Scientific), anti-β-actin (1:2000 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was incubated with 1/14 volume of free Protein G Dynabeads (ThermoFisher Scientific) rolling for 1 hour at 5°C to remove sticky proteins and RNAs ...
-
bioRxiv - Genetics 2022Quote: ... PDE6B protein was detected by Rabbit anti-PDE6B antibody (1:500; Thermo Scientific, PA1-722). Mouse anti-GAPDH antibody (1:10000 ...