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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... burgdorferi B31 genomic DNA with Taq polymerase and TA cloned into pCR2.1 (Thermo Fisher). Constructs were sequenced to guarantee sequence fidelity.
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bioRxiv - Microbiology 2020Quote: ... Borrelia burgdorferi genomic equivalents were calculated using an Applied Biosystems 7500 Real-Time PCR system (ThermoFisher Scientific) in conjunction with PowerUp SYBR Green Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... burgdorferi antibody (Invitrogen, USA ...
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bioRxiv - Immunology 2020Quote: ... burgdorferi polyclonal IgG (Invitrogen; CAT# PA1-73007 ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi monoclonal antibody (ThermoFisher, F21l) diluted in 1% normal goat serum (NGS ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi cells with TRIzol Reagent (Invitrogen, 15596026) according to the manufacturer’s instructions after a 30-minute 3,000 x g swinging-bucket centrifugation at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... then stained with a Borrelia polyclonal antibody diluted to 1:50 in 1x PBS (ThermoFisher, Waltham, MA, USA; FITC-PA1-73005) according to a previously established protocol [31] ...
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bioRxiv - Immunology 2020Quote: ... burgdorferi polyclonal IgG (Invitrogen; CAT# PA1-73007; Thermo-Fisher Scientific, Waltham, MA) at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi was purified using a GeneJET PCR Purification Kit (Thermo Scientific, #K0701), then sent for Eurofins sequencing and analyzed using the NCBI BLAST program (https://blast.ncbi.nlm.nih.gov/) ...
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bioRxiv - Microbiology 2023Quote: ... Larger fluorescently tagged fragments >60 bp were generated by PCR amplification of the target sequences from B31 genomic DNA and subsequent cloning into the pCR 2.1 TA vector (Invitrogen) following TOPO TA protocols ...
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bioRxiv - Microbiology 2022Quote: ... Individual samples were then screened using a duplex quantitative PCR for Borrelia burgdorferi and Borrelia miyamotoi in duplicate on an ABI 7500 Fast Real-Time PCR System using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and the resulting solution was used to perform multiplex PCR using primer pairs specific for each endogenous plasmid of strain B31 [61] and the DreamTaq Green DNA Polymerase (Thermo Scientific). For genomic DNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... burgdorferi cells harvested and washed as described above were resuspended in PBS+Mg without or with proteinase K (Invitrogen, 200 µg/ml final concentration). Proteinase K-containing and control samples were incubated for 1 h at room temperature ...
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bioRxiv - Genetics 2019Quote: ... and 5 µl of protein A: protein G 1:1 Dynabeads (Thermofisher). Antibody-bond chromatin was washed four times in low salt buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... A 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was prepared and 40 μL of the mixture was added to each well ...
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bioRxiv - Genomics 2020Quote: ... a protein A and protein G Dynabead mixture (1:1) (both from Invitrogen) was added to sonicated lysate along with 0.48 μg of c-Jun antibody (cat ...
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bioRxiv - Immunology 2021Quote: ... 40uL of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) were added to each well and then incubated at 4ºC for 4 hours on a rotator ...
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bioRxiv - Genomics 2019Quote: ... 110μL of a 1:1 Protein A and Protein G Dynabead slurry (ThermoFisher Scientific) was prepared by combining equal volumes of the Protein A and G beads and washing twice in low salt RIPA buffer with SDS (140mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... 15 μl of a 1:1 mix of Protein A and Protein G Dynabead (Invitrogen) was added followed by a 90 minute incubation at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 80,000 M-1·cm-1) against the protein concentration determined using Bradford protein assay (ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... 40 uL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) was added to each well and rocked at 4°C for 4 hours ...
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bioRxiv - Immunology 2021Quote: ... α-zona occludens-1 (ZO-1) protein (1:200, Invitrogen, 61-7300) and the lectins Ulex europaeus agglutinin - Atto488 conjugated (UEA ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Immunology 2019Quote: ... Protein A and Protein G coupled magnetic beads (mixture 1:1, Dynabeads, Thermo Fisher, 10002D and 10004D) were pretreated with BSA (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen) for 3 h at 4°C ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... were crosslinked to a 1:1 mixture of Dynabeads protein A and protein G (Thermo Fisher Scientific) with DMP and incubated with the supernatant at 4°C for 2 hr ...
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bioRxiv - Bioengineering 2022Quote: ... A 40 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for an additional 4 h at 4°C on a rotator (400 rpm) ...
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bioRxiv - Immunology 2023Quote: ... A 20 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for 4 h at 4°C with rotation (50 rpm) ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL pre-washed magnetic-conjugated protein G beads (Dynabeads protein G, Invitrogen) were added to the reactions followed by the incubation for 1 hour at 4°C in the thermomixer (750 rpm) ...
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bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
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bioRxiv - Biochemistry 2019Quote: The protein sample (∼1 μg) was mixed with Protein Thermal Shift Dye (Applied Biosystems) and 10∼10,000 μM concentration of each amino acid in 20 μL of assay buffer and loaded to a MicroAmpR Fast Optical 48-Well Reaction Plate (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... and rabbit polyclonal cementum protein 1 (1:100, Thermo Fisher Scientific) prepared in a solution of 0.3% Triton X-100 ...
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bioRxiv - Plant Biology 2024Quote: ... Mixture of Dynabeads with proteins A and G (1:1) (Invitrogen) was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-chromatin complexes were isolated using a 1:1 mixture of magnetic Protein A- and Protein G-dynabeads (ThermoFisher Scientific) and washed three times with a solution of 50 mM HEPES-KOH ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... Soluble chromatin supernatant equivalent to 6 μg per IP was pre-cleared using 20 μl of a 1:1 mix of protein A and protein G Dynabeads (Invitrogen). Overnight IP was performed using 1 μg of anti-H3K4me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... 1% of input chromatin was saved and 70 μg of chromatin pre-clearing was done by mixing with pre-blocked Sepharose beads (1:1 vol. mix of Protein A and protein G beads; Invitrogen) for 1h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... the phage:antibody complexes were immunoprecipitated using 40 μL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) (4°C for 4 hours ...
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bioRxiv - Pathology 2020Quote: ... and SARS Spike protein (Thermo Fisher, 1:250), and with Phalloïdin-647 (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and 1× Protein Thermal Shift dye (Applied Biosystems). In all experiments ...
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bioRxiv - Microbiology 2022Quote: ... and 1× Protein Thermal Shift dye (Applied Biosystems). The assay buffer (20 mM Tris pH 8.5 ...
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bioRxiv - Systems Biology 2023Quote: ... 1 mL of Protein G Dynabeads (ThermoFisher, #10003D) were washed once with 1X PBST (1X PBS + 0.1% Tween-20 ...
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bioRxiv - Genomics 2023Quote: 1 mL of Protein G Dynabeads (ThermoFisher, #10003D) were washed once with 1X PBSt (1X PBS + 0.1% Tween-20) ...
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bioRxiv - Molecular Biology 2024Quote: ... glial fibrillary acidic protein (GFAP; 1:50, Invitrogen), and EDC4 (1:40 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were then incubated for 1 hour with protein A-conjugated Dynabeads (Life Technologies), after which the beads were washed with lysis buffer at incremental KOAc concentrations (100 mM ...
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bioRxiv - Microbiology 2021Quote: ... 1 µl was used for protein quantification with the Pierce BCA Protein Assay Kit (Thermofisher). The remaining 9 µl were mixed with SDS (0.2% final) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mg protein for each condition was incubated with antibody-coated Dynabeads Protein G (Invitrogen, 1003D). Antibody dilutions are provided in table S3 ...
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bioRxiv - Biophysics 2022Quote: ... 1 μg of the dialyzed protein sample was mixed with Protein Thermal Shift Dye (Applied Biosystems) and 0.003 − 10 mM NaCl in 20 μL of buffer A ...