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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... glial fibrillary acidic protein (GFAP) (Invitrogen, California, USA, 1:2000 dilution) and with HRP (horse radish peroxidase)-conjugated goat anti-rabbit IgG (secondary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were alkylated by 1 mM Pierce N-ethylmaleimide (ThermoFisher Scientific) in alkylation buffer containing 8M urea ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5-1 mL of Protein A beads (Thermo Fisher Scientific, 89898) were added and gently rocked overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Proteins were digested with 1 µg MS-grade trypsin (Thermo Fisher) at 37°C overnight with gentle shaking (800 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... anti-glial fibrillary acidic protein (GFAP; 1:1500, MA5-12023, Invitrogen), anti-CD68 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected using GLUT4 (1:2000, PA5-23052, Invitrogen, USA), AKT(1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Protein was digested with 1 μg trypsin (Thermo Scientific, Waltham, MA) at 37 ºC overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Genetics 2020Quote: ... gRNA/Cas9 complexes were formed by incubating 250 ng of the gRNA with 1 µg of purified Cas9 protein (TrueCut Cas9 Protein v2, Invitrogen) in OptiMEM (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... per 1 mg of total protein were added along with 30 μL washed protein A/G UltraLink Resin (53133, ThermoFisher) and rotated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Biochemistry 2020Quote: ... The pellet was re-suspended in 5mL of 1% SDC + 50mM ABC pH 8.5 and protein concentration was measured with Pierce BCA protein assay kit (Thermo Scientific). Samples were then aliquoted into 1.5mL LoBind eppendorf tubes (Sorenson BioScience ...
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bioRxiv - Bioengineering 2022Quote: ... using a ratio of 1 µg trypsin to 50 µg sample protein as quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Samples were digested overnight at room temperature using a rotor for mixing and then placed in a speed vacuum to reduce sample volume ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... which were pre-conjugated to 1:1 mix of 63 µL protein A/G dynabeads (Invitrogen), and incubated (4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked using 5% w/v non-fat dry milk in TBST for one hour at room temperature and then incubated with primary antibody (anti-Alix, 1:5000; Protein Tech, 12422-1-AP or anA-FloAllin-1, 1:5000; Fisher Scientific, BDB610820) at 4°C overnight ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... frozen EVs were resuspended in RLT lysis buffer and RNAse-free water in a ratio of 1:3 and loaded based on protein concentration measured by Micro BCA Protein Assay Kit (Thermo Scientific). 2,8 μg of proteins were loaded.
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in 1% NP-40 lysis buffer (58) and protein levels were quantified using the Bicinchoninic acid protein assay (Thermo Scientific). Samples were run on SDS-PAGE gels before being transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates from 30 wt eye imaginal discs (1 mg of total protein) was precleared by incubating with protein A-sepharose beads (Thermo Scientific) for 1h at 4 °C followed by centrifugation ...
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bioRxiv - Biochemistry 2022Quote: ... and 150 µg of purified PPSA from SG and SEC fractions at 1 mg/mL total protein concentration (measured by a Pierce BCA protein assay, Thermo Scientific, according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% tri-tion-x-100) with 1% protein/phosphatase inhibitor (ThermoFisher; 78442). The RIPA lysate was sonicated and centrifuged for 15 min at 14,000 rpm at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and ∼1 µg primary antibody conjugated with protein-A dynabeads (Thermo Fisher) for EIF4A3-IP or CASC3-IP ...
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bioRxiv - Plant Biology 2019Quote: Machi3-1 protein was expressed with Pichia Expression Kit (Invitrogen K1710-01) using pPICz vector ...
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bioRxiv - Biochemistry 2022Quote: ... at 1:5000 dilution and MagicMark XP Western Protein Standard (Life Technologies).
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bioRxiv - Neuroscience 2021Quote: ... monoclonal mouse anti-S6 ribosomal protein (MA515123, Thermo Fisher Scientific; 1:100), polyclonal rabbit anti-RPL24 (PA530157 ...
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bioRxiv - Genetics 2020Quote: ... using a total of 1 μg of Cas9 protein (ThermoFisher Scientific, USA) per 0.5 ×105 cells.
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bioRxiv - Neuroscience 2020Quote: ... Rat anti-glial acidic fibrillary protein (GFAP; 1:1000, 13-0300, ThermoFisher), mouse anti-β-catenin (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... PAG1 (Csk-binding protein antibody PAG-C1) 1:1000 (ThermoFisher #MA1-19287); Phospho-Src family (Tyr416 ...
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bioRxiv - Cell Biology 2020Quote: ... and amyloid precursor protein (APP) at 1:500 (Invitrogen 14-9749-80). Imaging was performed with a BioRad ChemiDoc system and quantification was performed using ImageJ software.
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bioRxiv - Cell Biology 2020Quote: ... for 1 h with 40 μl of Dynabead-protein G (Life Technologies). Beads were washed with TSE-150 (0.1% SDS ...
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bioRxiv - Microbiology 2021Quote: ... Human alpha-1 PDX (alpha1-PDX) recombinant protein was purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... 5 μg of FGFb Recombinant Human Protein and 1% Gluta-MaxTM (Gibco) in flasks coated with Matrigel Matrix Basement Membrane (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 ng/mL Human TGF-β 1 recombinant protein (Cat# PHG9204, Gibco) and 1 μg/mL L-Ascorbic acid 2-phosphate (Cat# A8960 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg Invitrogen™ Protein G Dynabeads™ (Thermo Fisher Scientific, USA) was added to ten low protein binding microcentrifuge tubes ...
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bioRxiv - Genomics 2023Quote: ... 1 ug/mL Laminin mouse protein (Thermo Fisher Scientific; Cat. No. 23017015), 10 nM ROCK inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were then mixed with LDS loading buffer (Invitrogen NP0007; 1×) and reducing agent (Invitrogen NP0009 ...
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bioRxiv - Immunology 2022Quote: ... RNA-bound proteins were eluted by adding 1 μg RNaseA (Thermo Scientific) in 100 μl 100 mM Tris-HCl ...
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bioRxiv - Genomics 2022Quote: ... 1 µg/μL of TrueCut Cas9 protein V2 (ThermoFisher, Waltham, MA, USA), and 20% phenol red buffer (Sigma Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... The protein pellet was resuspended with Novex Nupage LDS buffer 1× (Invitrogen), and proteins were then denatured at 70 °C for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... WB 1:20 000) and Protein A HRP–conjugated were purchased from Invitrogen (WB 1: 20 000). The HALO-Trap Agarose beads and V5-conjugated beads were purchased from ChromoTek and Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... WT RBA1 (residues 1-363) and RBA1 mutant proteins were cloned into the pFastBac 1 vector (Invitrogen) with an N-terminal SUMO-6×His tag ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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bioRxiv - Molecular Biology 2022Quote: ... was preincubated with 1:1 mixture of washed Dynabeads Protein A and G (1002D and 1004D, Invitrogen) for more than 30 min at room temperature and washed twice with lysis buffer to remove unbound antibodies ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature for all target proteins or with clear milk blocking buffer for β-actin protein (Pierce, Thermo Scientific). We used the following LARP1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody-bound NLGNs were incubated for 1 hour with 20 µL of protein G beads (Dyna-beads Protein G, Thermo Fisher Scientific) precipitated and washed 4 times with lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... microsphere mixtures were successively incubated with serum samples (1:400) biotinylated protein A and biotinylated protein G (4 μg/ml each) (Thermo Fisher Scientific), and Streptavidin-R-Phycoerythrin (4 μg/ml ...