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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Primers were amplified using 2X SYBR Green Reagent (Life Technologies, 4364346) using a BioRad CFX384 Touch Real Time PCR Detection System ...
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bioRxiv - Cancer Biology 2024Quote: ... along with gene specific primers using a QuantStudio 3 (Applied Biosystems). Primers used are listed in Supplemental Table 1.
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bioRxiv - Neuroscience 2024Quote: ... All primers and probes were custom made and purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2024Quote: ... Oligonucleotide primers and MGB-labelled probes were synthesized by Invitrogen (USA). Amplifications were performed using TaqMan Universal Master Mix II (catalogue no ...
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bioRxiv - Microbiology 2024Quote: ... Oligonucleotide primers and MGB-labelled probes were synthesized by Invitrogen (USA). Extensions were performed according to a standard protocol ...
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bioRxiv - Physiology 2024Quote: ... The following pre-made Mus musculus primers (Applied Biosystems, catalog #4331182) were used in the qRT-PCR panel ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-mouse Alexa-Fluor 488, 1:500, Thermofisher Scientific, 4 hrs at RT; Th: goat anti-rabbit Alexa-Flour 488, 1:500, Thermofisher Scientific, 4 hrs at RT). Sections were mounted on gelatin subbed slides and coverslipped with VectaShield anti-fade mounting medium (Vector Labs).
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bioRxiv - Neuroscience 2024Quote: ... The primers in Table 1 were used for RT-qPCR reactions and RT-qPCR reactions were loaded into MicroAmpTM Fast Optical 96-Well Reaction plates (Thermo Fisher Scientific 43-469-07) sealed with optical adhesive film (Thermo Fisher Scientific AB-1170) ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR was performed using the PCR Power SYBR Green PCR Master Mix (Applied Biosystems) in a B7900-FAST-384 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were cloned into pCR-Blunt vector (ThermoFisher,), transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned into pCR 4.1 TOPO (Invitrogen), transformed into Chemically Competent E ...
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bioRxiv - Neuroscience 2024Quote: ... 10 ng of miRNA underwent reverse transcription to cDNA using the Taqman™□ MicroRNA Reverse Transcription Kit and the Taqman™□ MicroRNA Assay (miR-200a-3p: 000502, and U6 snRNA: 001973; Thermo Fisher Scientific Inc., USA). Subsequently ...
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bioRxiv - Bioengineering 2020Quote: ... then integrated into single-control and single-experimental sets before homogenization in TRIzol reagent (Invitrogen, ThermoFisher). Following homogenization ...
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bioRxiv - Bioengineering 2020Quote: ... then integrated into single-control and single-experimental sets before homogenization in TRIzol reagent (Invitrogen, ThermoFisher). Following homogenization ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were mounted in SlowFade™ Glass Soft-set Antifade Mountant (Thermo Fisher Scientific Cat# S36917) and sealed with CoverGrip™ Coverslip Sealant (Biotium Cat# 23005) ...
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bioRxiv - Immunology 2020Quote: Total serum IgE was quantified using a Mouse IgE ELISA Ready-SET-Go! kit (eBio/Affymetrix). HES-specific IgG1 was quantified using a Mouse IgG1 ELISA Ready-SET-Go! kit (eBio/Affymetrix) ...
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bioRxiv - Immunology 2020Quote: ... HES-specific IgG1 was quantified using a Mouse IgG1 ELISA Ready-SET-Go! kit (eBio/Affymetrix). HES was prepared as previously described9 and a 96-well flat-bottom plate was coated with 1µg/mL and incubated at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... one set of 14 µl aliquots was resolved on 15% TBE-urea polyacrylamide gel (Life Technologies) at 150 V for 1 hrs and 20 min at room temperature in 1x TBE buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then fixed and permeabilized with either eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen) or Fixation/Permeabilization Solution Kit (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... with one half fixed and permeabilized with either eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen), and the other half kept alive in PBS with 2% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Analysis of intracellular antigens was achieved using the Foxp3 / Transcription Factor Staining Buffer Set (ThermoFisher Scientific). Following staining ...
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bioRxiv - Immunology 2019Quote: ... Fixation and permeabilization were performed using the Foxp3 Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) according to the manufacturer’s suggestions followed by intracellular cytokine and CD154/CD40-L staining ...
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bioRxiv - Cancer Biology 2019Quote: ... Intracellular staining for FOXP3 was performed using the eBioscience FOXP3/Transcription Factor Buffer Set from Invitrogen and stained with the FOXP3 (MF23 ...
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bioRxiv - Bioengineering 2021Quote: ... for 30 min then fixed and permeabilized with eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen). Samples were next Fc-blocked for 10 min using TruStain fcX (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: Neurons were manually reconstructed for all three SBEM data sets using both Amira (Thermo Fisher Scientific) and CATMAID software (Saalfeld et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Each read was set up in triplicate on a white polystyrene 96-well plate (ThermoFisher 236105) and to each well was added 80 µL Stabilizyme ...
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bioRxiv - Neuroscience 2020Quote: ... Gates were set based on FS vs SS and viability dye exclusion (LIVE/DEAD Aqua, Invitrogen). Microglia were identified as CD11b/c+/CD45lo/CD90.1- cells and monocytes identified as CD11b/chi/CD45hi/CD90.1- cells ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed and permeabilized with the eBioscience FOXP3/Transcription Factor Fixation/Permeabilization buffer set (ThermoFisher) and then subjected to intracellular staining with anti- C1Q (RmC7H8) ...
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bioRxiv - Developmental Biology 2022Quote: ... (probe sets are presented in Supplementary Table T1) and dissolved in Nuclease-Free Distilled Water (Invitrogen). HCR amplifiers with B1-Alexa Fluor-546 ...
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bioRxiv - Immunology 2020Quote: Whole blood for all sample sets were collected into Tempus Blood RNA Tubes (Thermo Fisher Scientific). Total RNA was isolated from whole blood lysate using a MagMAX for Stabilized Blood Tubes RNA Isolation Kit for Tempus Blood RNA Tubes (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... All reaction conditions were set up in triplicate with ABsolute Blue QPCR Mix (Thermo Fisher Scientific) and then run on an ABI StepOne Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining for transcription factors was performed using the Foxp3 Staining Buffer Set (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... cells were fixed and permeabilized using eBioscienceTM FoxP3/transcription factor staining buffer set (Thermo Fisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Epidemiology 2019Quote: ... The heme metabolism gene set was excluded due to the globin interference artefact (communication with ThermoFisher).
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bioRxiv - Neuroscience 2020Quote: ... Mouse TNF-α and IL-6 ELISA Ready-SET-Go! kits (Affymetrix, San Diego, CA, USA) were used for the detection of tumor necrosis factor α (TNF-α ...
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bioRxiv - Cancer Biology 2020Quote: ... Tryptic peptides were labeled using a tandem mass tag 10-plex isobaric label reagent set (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Each read was set up in triplicate on a white polystyrene 96-well plate (ThermoFisher 236105) and to each well was added 80 μL Stabilizyme ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PACE was set up in a 37 ⍛C environmental chamber (Forma 3960 environmental chamber, ThermoFisher Scientific). For each replicate ...
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bioRxiv - Neuroscience 2021Quote: ... On-column DNaseI digestion was performed using the PureLink™ DNase Set (Thermo Fisher Scientific, #12185010). cDNA was synthesized using the iScript™ Reverse Transcription Supermix kit (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The first electrode (6020RS) was set to +600 mV and placed before the column (Thermo Scientific, Betasil C18 ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining for transcription factors was performed using the Foxp3 staining buffer set (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience / Thermo Fisher Scientific), according the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... & gene expression assessed using TaqMan Fast Master Mix & and TaqMan FAM-MGB probe sets (Applied Biosystems): Gapdh ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Thermo Fisher, 88-8824). A first intracellular staining with anti-Fc AF647 was performed prior to washing twice with 1X permeabilization buffer ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the eBioscience FOXP3/Transcription factor staining buffer set from Invitrogen.
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed and permeated with a transcription factor staining buffer set (Invitrogen 00-5523-00) following staining for extracellular proteins ...
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bioRxiv - Microbiology 2022Quote: ... Reporter reactions were set up in a 384-well black plate (Thermo fisher, Waltham, MA, USA) and incubated in an Enspire fluorescence plate reader (Perkin Elmer ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8.5 and labelled with 0.5 mg TMTpro 16plex Isobaric Label Reagent Set (Thermo Scientific A44522). Individually labelled samples were combined and desalted using a Pierce Peptide Desalting Column (Thermo Scientific 89851) ...
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bioRxiv - Molecular Biology 2022Quote: ... We set up qPCR reactions using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, #A25741). For cell lines that required the touchdown qPCR protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were then fixed and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (Thermo Fisher), and subsequently stained for intracellular and intranuclear proteins ...