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Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The primer sequences were synthesized using Custom DNA Oligos Synthesis Services (Thermofisher). We used a Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... Fragmented mRNA was converted into cDNA using random primers and SuperScriptII (Invitrogen). This was followed by second strand synthesis ...
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bioRxiv - Biophysics 2022Quote: ... Excess primers were removed by adding 0.04 uL of ExoSAP-IT (Affymetrix) per uL of PCR1 reaction and incubated for 20 min at 37 ̊C followed by an inactivation for 15 min at 80 ̊C ...
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bioRxiv - Biochemistry 2023Quote: ... and cDNA synthesised using oligo(dT) primers and SuperScript IV (ThermoFisher Scientific). Target genes were then cloned into pCellFree_03 using the Gibson assembly protocol (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... for 60 min and reverse-transcribed using random primers (Thermo Fisher Scientific) and SuperScript III (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... reverse transcriptase using Oligo(dt)20 primers (Thermo Fisher, Cat.No.18418-020).
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bioRxiv - Cell Biology 2023Quote: ... The cDNA was synthesized by using random hexamer primers (Thermo Fisher Scientific). Primers were annealed for 10 min at 25 °C followed by cDNA synthesis using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and 250 ng oligo(dT)12-18 primers (ThermoFisher, Waltham, MA, USA). The resulting complementary DNA (cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... Cutsom-made primers with probes for TaqMan were purchased from Thermo Fisher: housekeeping gene ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed with random primers and Superscript IV (Life Technologies) and quantitative PCR with the Power SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: ... was combined with primers and gDNA in 48-well plates (Thermo Scientific) and samples were analyzed using a StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... 250 nM primers and Fast SYBR™ Green Master Mix (Applied Biosystems) using the StepOnePlus Real-time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... MgCl2 and forward and reverse primers using a QuantStudioTM 12KFlex (Applied Biosystems). Appropriate controls without reverse transcriptase or with H2O instead of cDNA were performed ...
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bioRxiv - Cancer Biology 2024Quote: ... with the following TaqMan primers: St3Gal1 (Thermo Fisher Scientific Assay ID: Mm00501493_m1); St6Gal1 (Thermo Fisher Scientific Assay ID ...
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bioRxiv - Genetics 2024Quote: ... The forward primer was fluorescently labeled with 6-FAM (Thermo Fisher Scientific) and products were resolved using the ABI 3730xl DNA analyzer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR was performed using quantitative real-time PCR (PikoReal real-time PCR, Thermo Fisher Scientific). PCR (three replicates ...
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bioRxiv - Molecular Biology 2020Quote: PCR reactions were performed on a Real-time PCR System (7500 Real-time PCR System, Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... in GeneAmp PCR 9700 thermocycler and qRT-PCR amplification with TaqMan Universal PCR Master Mix (Applied Biosystems). All qRT-PCR measurements were obtained in a 7900HT Fast Real Time PCR System with ExpressionSuite Software v1.0 (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... PCR products were cloned into the pCR-XL vector using the TOPO XL PCR cloning kit (Invitrogen). Chemically competent Top10 E ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR (Q-PCR) was performed using Power SybrGreen PCR master-mix (4367659, Applied Biosystems, Warrington, UK) in an Applied Biosystem 7500 Fast Real Time PCR instrument ...
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bioRxiv - Cancer Biology 2023Quote: Quantitative reverse transcription-PCR (qRT-PCR) analysis was done on a QuantStudio Real-Time PCR instrument (ThermoFisher) using PowerUp™ SYBR® Green Master Mix (ThermoFisher) ...
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bioRxiv - Genetics 2021Quote: ... PCR was performed on a ProFlex PCR System (Thermo Fisher) under the following cycling conditions ...
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bioRxiv - Cell Biology 2019Quote: ... PCR was performed with GeneAmp PCR System 9700 (Applied Biosystems) using FastStart Taq DNA polymerase (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR was performed with GeneAmp PCR System 9700 (Applied Biosystems) using FastStart Taq DNA polymerase (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... followed by PCR product purification (PureLink PCR Purification Kit, Invitrogen) and cloning into the XmnI/HindIII restriction sites of pMAL-c2 vector ...
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bioRxiv - Cell Biology 2021Quote: ... PCR product was cloned into pCR-Blunt II-TOPO (Invitrogen) by blunt-end ligation according to manufacturer’s instructions and transformed into DH5α cells ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were performed using Platinum SuperFi PCR Master Mix (Invitrogen) or repliQa HiFi ToughMix (Quantabio) ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed using PCR Master Mix (2x) (Thermo Scientific) in a 3PRIME Thermal Cycler (TECHNE ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting PCR product was cloned into pCR-TOPO (Thermofisher) and subsequently removed by digestion with MunI and BamHI ...
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bioRxiv - Cancer Biology 2023Quote: ... or 50nM miR-1207-3p oligonucleotide mimic (MISSION® microRNA Mimic, product# HMI0066) for 48-72 hrs in Opti-MEM (Thermo Fisher Scientific Inc; Wilmington, DE, U.S.A) combined with complete media.
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bioRxiv - Synthetic Biology 2020Quote: ... pCR-blunt (Invitrogen). The ‘Cell-free to Clostridium’ vectors were derived from pJL1 plasmid (Addgene #69496) ...
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bioRxiv - Developmental Biology 2021Quote: ... 20-μl reactions were set up containing 10 μl of TaqMan 2× Universal Master Mix (Applied Biosystems), 1 μl 20× 6-carboxyfluorescein (FAM)-labeled Assay Mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were fixed by using the Foxp3/transcription factor staining buffer set (Thermo Fisher Scientific, Waltham, MA) followed by intracellular staining ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a final set of washes and mounting with Fluoromount-G (Thermo Fisher Scientific, Wilmington, DE) or prepared polyvinyl acetate (PVA).
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bioRxiv - Plant Biology 2021Quote: ... Normalized probe set signals in the log2 scale were calculated by using the RMA algorithm (Applied Biosystems GeneChip Expression Console v1.4 software ...
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bioRxiv - Microbiology 2021Quote: The medium throughput screen was set up in sterile 96-well flat bottom polystyrene plates (Nunc: 260860). For each assay ...
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bioRxiv - Microbiology 2021Quote: ... Desalted peptides were labeled with TMT 6-plex reagents (TMT6plex™ Isobaric Label Reagent Set, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Released IL-1β was quantified using the Human IL-1 beta ELISA Ready-Set-Go! Kit (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed/permeabilized using the eBioscience™ Foxp3/ Transcription Factor Staining Set (00-5523-00, Invitrogen). For cytokine staining ...
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bioRxiv - Genomics 2022Quote: ... and a two-column set up with a C18 PepMap100 trap-column (5μm, 300μm i.d.x 5 mm, 100Å, Thermo Scientific) and a 25 cm analytical column (EASY-Spray PepMap C18 ...
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bioRxiv - Biophysics 2022Quote: ... the chamber set at 12 °C and 95% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... into 50 micron sections and permeabilized overnight using the Foxp3 Transcription Factor Staining Buffer Set (Thermo Fisher). Permeabilized sections were stained in the supplied permbuffer with an antibody against RORγt (AFKJS-9 ...
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bioRxiv - Cancer Biology 2022Quote: ... with 10% (or 20% for CA46 and SET-2 cells) FBS (Thermo Fisher Scientific, #10-437-028) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... CuAAC reaction was set up following the instructions from the Click-iT Protein Enrichment Kit (Invitrogen, C10416), but with the provided beads substituted for azide agarose beads (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were spun and fixed using the eBioscience Intracellular Fixation & Permeabilization Buffer Set (ThermoFisher, 88-8824-00) according to manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: Chemical labelling of peptide samples was performed using TMT10Plex™ Isobaric Label Reagent Set (ThermoFisher Scientific, USA). 0.2 mg of TMT tag was used to label each sample (Table S1) ...
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bioRxiv - Immunology 2022Quote: ... fixed and permeabilized by using Ebioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Scientific 00-5523-00) kit according to manufacture instructions ...
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bioRxiv - Microbiology 2019Quote: Amounts of IFNγ and IL-6 in BALF were estimated by ELISA Ready-SET-Go! Kit (Invitrogen) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... we carried out data analysis of differentially expressed genes (DEGs) of 26,966 probe sets (p.s.) from Affymetrix MoGene 1.0 ST microarray data from mouse mucosa scrapings produced in our previous study (15) ...
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bioRxiv - Immunology 2020Quote: ... eBioscience) using the eBioscience Foxp3/Transcription factor staining buffer set according to the manufacturer’s instructions (ThermoFisher Scientific).