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Citations for Thermo Fisher :
4901 - 4950 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and ~200 ng of peptides were injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting peptides were desalted and analyzed by LC-MS/MS on a Q-Exactive plus mass spectrometer (Thermo-Fisher scientific, Pittsburgh, PA) fitted with a capillary HPLC (easy nLC 1000 ...
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bioRxiv - Biochemistry 2022Quote: ... Labeled peptides were first trapped on an Acclaim™ PepMap™ 100 C18 precolumn (5 μM, 0.3 mm X 5 mm, Thermo Fisher Scientific) and eluted to the analytical column nanoEase M/Z Peptide BEH C18 Column (130Å ...
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bioRxiv - Neuroscience 2021Quote: ... Mass-spectrometry-based quantification of CaMKIIα/β-derived peptides was carried out by selected reaction monitoring (SRM) analysis using a TSQ Quantiva triple-stage quadrupole mass spectrometer (Thermo Fisher Scientific, U.S.A.). The following parameters were selected ...
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bioRxiv - Molecular Biology 2021Quote: ... One microgram peptides for global proteomics or all the enriched phosphorylated peptides were loaded and concentrated on an Acclaim PepMap 100 C18 precolumn (75 μm x 2 cm, Thermo Scientific, Waltham, MA) and then separated on an Acclaim PepMap RSLC column (75 μm x 25 cm ...
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bioRxiv - Cancer Biology 2020Quote: The PulseDIA acquisition of HeLa peptides was performed on a nanoflow EASY-nLC™ 1200 System (Thermo Fisher Scientific™, San Jose, USA) coupled to a Q Exactive HF-X hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific™ ...
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bioRxiv - Molecular Biology 2019Quote: ... Eluted peptides were analyzed on a Q-Exactive Plus mass spectrometer or an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). The software Scaffold 4.7.2 (Proteome Software Inc. ...
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bioRxiv - Biochemistry 2019Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany) and stored at −80 °C.
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bioRxiv - Cancer Biology 2019Quote: ... The collected peptide was desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Microbiology 2021Quote: ... NanoUPLC-hr MS / MS analysis of the resulting peptide mixtures were performed on a Q-Exactive orbitrap mass spectrometer (Thermo Fisher Scientific, USA), coupled with a nanoUltimate300 UHPLC system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Tagged tryptic peptides (~250 μg) were subjected to strong cation exchange fractionation using a hand-held ICAT® Cartridge-cation-exchange system (Applied Biosystems, USA). Peptides were eluted using a gradient 30 ...
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bioRxiv - Microbiology 2020Quote: 50µg of peptides from each sample were labeled with isobaric tandem-mass-tag (TMT) 11 plex reagents (Thermo Fisher Scientific, San Jose, CA) in 20 mM pH 8.5 HEPES with 30% acetonitrile (v/v ...
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bioRxiv - Microbiology 2020Quote: The flow-through fraction of each TMT11 experiment was fractionated into 13 fractions using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... the UV absorbance of the NPs was measured at 205 nm before and after peptide coupling using a spectrophotometer (Nanodrop 2000, Thermo Fisher Scientific, UK) and the difference of the absorbance (ΔA205 ...
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bioRxiv - Molecular Biology 2020Quote: ... The separated peptides were then electrosprayed in the positive ion mode into an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher, San Jose, CA, USA) operated in data-dependent acquisition mode (3 s cycle time) ...
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bioRxiv - Biochemistry 2020Quote: ... and with or without CLASPIN pS945 peptide (MEELLNLC(pS)GKFTSQ) (Peptide Protein Research Ltd, Bishops Waltham, UK) at different concentrations in a Thermoscientific 96 well UV microplate (Fisher scientific, Loughborough, UK). The plates were incubated at 25 °C and absorbance at 340 nm was measured in a POLARstar Omega multimode microplate reader (BMG Labtech GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide samples of EMARS products prepared as well as mouse samples were injected into an Ultimate 3000 RSLCnano (Thermo Fisher Scientific, MA). Mass analysis was performed on a LTQ Orbitrap XL mass spectrometer equipped with a nano-ESI source (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... operated in the cone-jet spraying regime for efficient ion generation.33 Peptide ions were detected using a quadrupole orbitrap mass spectrometer (Q Exactive Plus, Thermo Scientific, Waltham, MA) between m/z 350–1,800 at 35,000 FWHM resolution (128 ms transient ...
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bioRxiv - Neuroscience 2021Quote: Eluted peptides were ionized by a nanospray source and detected by an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operating in a data dependent mode (DDA) ...
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bioRxiv - Cancer Biology 2020Quote: ... Tagged tryptic peptides (∼250 μg) were subjected to strong cation exchange fractionation using a hand-held ICAT® Cartridge-cation-exchange system (Applied Biosystems, USA).
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid ...
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bioRxiv - Plant Biology 2020Quote: The coding DNA sequence for human Factor IX without the native signal peptide was synthesized as N-terminal translational fusion to the signal-peptide coding sequence of PpAP1 (Pp3c5_19520V3.1)42 by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA). It was cloned into the pAct5-MFHR1 plasmid41 to generate the final FIX expression vector ...
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bioRxiv - Systems Biology 2020Quote: ... fractionation for diGly peptide and proteome spectral libraries were performed on an UFLC system (Shimadzu) and EASY-nLC 1000 (Thermo Fisher Scientific, Germany), respectively.
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bioRxiv - Systems Biology 2021Quote: The concatenated and TMT-labelled peptide mixtures were analyzed using nanoflow liquid chromatography (LC-MS/MS) on an EASY nano-LC 1200™ system (Thermo Fisher scientific), connected to a Q Exactive HF (Thermo Fisher scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Air dried peptides were analyzed using a nanoflow liquid chromatography (LC-MS/MS) on an EASY nano-LC 1200™ system (Thermo Fisher scientific), connected to a Q Exactive HF (Thermo Fisher scientific ...
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bioRxiv - Microbiology 2020Quote: Liquid chromatography coupled to mass spectrometry (LC-MS/MS) analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... The soluble fraction was then added to a new tube and the peptides were extracted and desalted using C18 desalting columns (Thermo Scientific, PI-87782). Peptides were quantified using BCA assay and a total of 1 ug of peptides were injected for LC-MS analysis.
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bioRxiv - Microbiology 2021Quote: ... and then tryptic peptides were subjected to LC-MS/MS on an Easy-Nlc 1000 coupled with Q Exactive Orbitrap instrument (Thermo Fisher Scientific, USA) under standard conditions ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting peptides were then labelled with TMT ten-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled and desalted using a SepPak cartridge according to the manufacturer’s instructions (Waters ...
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bioRxiv - Microbiology 2021Quote: ... isotopologue patterns of labeled peptides were extracted manually from mass spectral data using the Xcalibur Qual Browser (v3.0.63, Thermo Fisher Scientific, Waltham, MA, USA), and 13C incorporation was calculated as previously described24.
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bioRxiv - Plant Biology 2021Quote: ... Five microliters of peptide mixtures were loaded onto an Acclaim PepMap™ precolumn (75 μm × 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm × 50 cm ...
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bioRxiv - Microbiology 2021Quote: LC-MS/SM analysis of trypsin-digested proteins (peptides) was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... dried at 30°C in a vacuum concentrator and fractionated using a Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were then labeled with TMT eleven-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled and desalted using a SepPak cartridge according to the manufacturer’s instructions (Waters ...
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bioRxiv - Biophysics 2022Quote: ... Peptides were dissolved in 25 μl 0.1% trifluoroacetic acid and 6 μl of the extracted peptides was analyzed by Orbitrap Q Exactive HF-X (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Biochemistry 2020Quote: LC-MS/MS analyses of all peptide mixtures were done using UltiMate™ 3000 RSLCnano system connected to Orbitrap Fusion Lumos Tribrid spectrometer (Thermo Fisher Scientific). Prior to LC separation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The eluted peptides from LC were subjected to mass spectrometry using a Q Exactive HF-X Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany). Protein identification was performed using Proteome Discoverer 2.4 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were delivered onto a nano RP column (5-μm Hypersil C18, 75 mm × 100 mm; Thermo Fisher Scientific, Waltham, MA, USA) and eluted with escalating (50-80% ...
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bioRxiv - Biochemistry 2021Quote: ... Desalted peptides were injected into an Easy-nLC 100 HPLC system coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Bremen, Germany). Peptide samples were loaded onto an Acclaim PepMapTM 100 C18 trap column (75 μm × 20 mm ...
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bioRxiv - Immunology 2021Quote: Cells were stimulated with 2 μg/mL of MTB300 epitope peptide pool (A&A Labs, USA) in the presence of brefeldin A and monensin (Thermo Fisher Scientific, USA) for 6 hours at 37°C in Complete media (RPMI-1640 with 10% fetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2022Quote: Protein digestion was performed according to the standard procedure and the resulting peptide mixture was labeled using the 10-plex TMT reagent (Thermo fisher, Art.No.90111) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... to the following protocol: Analysis of peptide readout was performed on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (Thermo Scientific) coupled with a Dionex Ultimate 3000 RS (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Tryptic peptides were directly injected to an EASY-Spray analytical column (2 μm, 100 Å PepMapRSLC C18, 25 cm × 75 μm, Thermo Fisher Scientific) operated at constant temperature of 35 °C ...
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bioRxiv - Cell Biology 2019Quote: ... a total of 1 ug of peptides was injected into a Dionex Ultimate 3000 NanoRSLC instrument with a 15-cm Acclaim PEPMAPC18 (Thermo Fisher Scientific #164534) reverse phase column ...
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bioRxiv - Biochemistry 2019Quote: ... These digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Synthetic Biology 2020Quote: Retention time was predicted after standard runs with the above-described method using the Pierce™ Peptide Retention Time Calibration Mixture (Catalog number 88320, Thermo Scientific, USA).
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bioRxiv - Cell Biology 2020Quote: ... the same maximal quantity of peptides was used for TMT labelling using a TMT10plex™ Isobaric Label Reagent Set (#90110, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The peptides were loaded on a 50 cm EASY-Spray™ column (75 μm inner diameter, 2 μm particle size, Thermo Fisher Scientific), equipped with an integrated electrospray emitter ...
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bioRxiv - Microbiology 2021Quote: ... PM-WCPV-S) or N peptide pool (Meltenyi, 130-126-698) in the presence of 1 µg/mL anti-CD28 (Invitrogen, 14-0281-86) for co-stimulation for 6 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were then analyzed with an nanoLC-MS/MS system (Ultimate nano-LC and LTQ Velos ion trap, Thermo Scientific, San Jose Califronia). Briefly ...